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Objective: To evaluate the influence of opacity and the layering technique on the fluorescence of different composite resins. Materials and Methods: Two opacities (enamel and dentin) and the layering technique (enamel + dentin) of the composite resins: Filtek® Z350 and Palfique LX5 were evaluated in vitro. Composite resin discs were fabricated using a preformed matrix of 10 mm diameter and 0.5 mm thick for the single opacity groups and 10 mm thick for the layering technique groups, using 2 layers of 0.5 mm thickness of each opacity (n = 5). Specimens were analyzed using the Raman spectroscopy method. Data were analyzed using the Kruskall-wallis and Mann-Whitney U tests. Results: When evaluating the intensity of fluorescence, no statistically significant difference was found when comparing the layering technique and enamel opacity (p2> 0.05) and an increase in the dentin opacity value for both brands of composite resin. Regarding wavelength, no statistically significant difference was found when comparing the layering technique with enamel opacity and dentin opacity for both Filtek® Z350 and Palfique LX5® composite resins (p2 > 0.05). Conclusions: The fluorescence intensity of the layering technique is similar to enamel opacity for both composite resins. Likewise, the wavelength of the layering technique is similar to the enamel opacity and dentin opacity for both brands.
Objetive: Evaluar la influencia de la opacidad y de la técnica de estratificación en la fluorescencia de diferentes resinas compuestas. Materiales y Métodos: Se evaluó in vitro 2 opacidades (Esmalte y Dentina) y la técnica de estratificación (Esmalte + Dentina) de las resinas compuestas: Filtek® Z350 y Palfique LX5. Se fabricaron discos de resina compuesta, utilizando una matriz preformada de 10 mm de diámetro y 0,5 mm de grosor para los grupos de opacidad única y 10 mm de grosor para los grupos de técnica estratificada, utilizando 2 capas de 0,5 mm de cada opacidad (n = 5). Los especímenes se analizaron mediante el método de Espectroscopía Raman. Los datos se analizaron utilizando la prueba de Kruskall-wallis y Prueba U de Mann Whitney. Resultado: Al evaluar la intensidad de fluorescencia no se encontró diferencia estadísticamente significativa entre los pares: Técnica estratificada versus Opacidad Esmalte para ambas marcas de resina compuesta Filtek® Z350 y para Palfique LX5® (p2 > 0,05). Para longitud de onda no se encontró diferencia estadísticamente significativa entre los pares: Técnica estratificada versus Opacidad Esmalte y Técnica estratificada VS Opacidad Dentina para ambas resinas compuesta Filtek® Z350 y Palfique LX5® (p2> 0,05). Conclusión: La intensidad de fluorescencia de la técnica estratificada es similar a la opacidad Esmalte para ambas resinas compuestas. De igual manera la longitud de onda de la técnica estratificada es similar a la opacidad Esmalte y opacidad Dentina para ambas marcas.
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Humanos , Resinas Compuestas/química , Nanotecnología/métodos , Análisis Espectral , Técnicas In VitroRESUMEN
Abstract This review provides the current state of knowledge regarding the use of nutritional nanocompounds on exercise performance. The reviewed studies used the following nanocompounds: resveratrol-loaded lipid nanoparticles, folic acid into layered hydroxide nanoparticle, redox-active nanoparticles with nitroxide radicals, and iron into liposomes. Most of these nutritional nanocompounds seem to improve performance in endurance exercise compared to the active compound in the non-nanoencapsulated form and/or placebo. Nutritional nanocompounds also induced the following physiological and metabolic alterations: 1) improved antioxidant activity and reduced oxidative stress; 2) reduction in inflammation status; 3) maintenance of muscle integrity; 4) improvement in mitochondrial function and quality; 5) enhanced glucose levels during exercise; 6) higher muscle and hepatic glycogen levels; and 7) increased serum and liver iron content. However, all the reviewed studies were conducted in animals (mice and rats). In conclusion, nutritional nanocompounds are a promising approach to improving exercise performance. As the studies using nutritional nanocompounds were all conducted in animals, further studies in humans are necessary to better understand the application of nutritional nanocompounds in sport and exercise science.
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@#Cancer diagnosis and treatment has always been a hot spot in clinical and basic scientific research. In recent years,scientists have developed a large number of tumor diagnosis and treatment methods based on bacteria combined with nanotechnology. Compared with pure bacterial diagnosis and treatment,bacterial diagnosis and treatment combined with nanotechnology can produce multiple synergistic effects,thereby improving the efficacy of tumor diagnosis and treatment.The characteristics of bacteria such as environmental sensitivity,tropism,motility and hypoxia growth combined organically with nanotechnology can increase the solubility of insoluble drugs,promote drug lysosomal escape,and avoid phagocytosis and clearance of the reticuloendothelial system to construct a new type of bacterial micro/nano diagnosis and treatment platform,thereby achieving the precise tumor diagnosis and controlled drug release. This paper reviewed the research progress of bacteria combined with nanotechnology for tumor diagnosis and treatment in recent years and the challenges and possible solutions,so as to provide reference for promoting the rapid development of tumor diagnosis and treatment research.
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Atherosclerosis(AS)is a common cardiovascular disease,and its treatment and prevention have been the focus of medical research.AS an emerging technology,nanotechnology has unique advantages and plays an important role in the prevention,diagnosis and treatment of AS.This paper reviews the latest research on the application of nanotechnology in AS diseases,systematically discusses the role of nanotechnology in the diag-nosis and treatment of AS,and comprehensively analyzes the effects of nano-drug carriers based on different sur-face trimmers,loading diagnostic and therapeutic drugs so as to monitordisease progression of AS and its targeted treatment.The aim is to provide new thought for the clinical treatment of AS.
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BACKGROUND:Photothermal therapy is a novel tumor treatment strategy that uses photothermal agents to transform light energy into heat energy to accomplish non-invasive tumor ablation.The rise of photothermal therapy and nanotechnology has provided a new perspective on breast cancer treatment.OBJECTIVE:To prepare a new type of near-infrared biomimetic nanoprobe that has been modified by breast cancer cell membrane,to investigate the effect of near-infrared fluorescence/ultrasound imaging in vitro,and to observe its targeting ability and photothermal therapy effect on homologous tumor cells in vitro.METHODS:Organic small molecule ITIC-4CI with A-D-A structure was used as photothermal agents;polylactic acid/glycolic acid copolymer as nanocarrier;4T1 cell membrane of mouse breast cancer cells as a surface modifier of nanoparticles;perfluorohexane(PFH)was loaded.A novel near-infrared biomimetic nanoprobe(4T1m/ITIC-4CI/PFH)was prepared by the double emulsion evaporation method and sonication method.The basic characterization of the nanoprobe and the homologous targeting ability were detected.The photothermal properties and photothermal stability of the probe were investigated,and the near-infrared fluorescence/ultrasound imaging effect of the probe under laser irradiation was observed.The CCK-8 assay and calcein/propidium iodide staining were used to assess the efficacy of photothermal therapy.RESULTS AND CONCLUSION:(1)The prepared 4T1m/ITIC-4CI/PFH nanoprobes had uniform size,high stability,and an average particle size of(92.7±2.3)nm.The probe's protein composition was identical to that of the 4T1 cell membrane.The nanoprobe's ability to target homologous 4T1 cells was validated by an in vitro cell uptake assay.(2)The nanoprobe had a red-shift absorption spectrum and tail emission extending to the near-infrared-Ⅱ,which emitted a bright near-infrared-Ⅱ fluorescence signal under laser irradiation.(3)After laser irradiation,the nanoprobe 4T1m/ITIC-4CI/PFH could be turned into microbubbles and enhanced ultrasound imaging.The results of CCK-8 assay and calcein/propidium iodide staining showed that the nanoprobe 4T1m/ITIC-4CI/PFH had an obvious photothermal killing effect on 4T1 cells.(4)The results show that the nanoprobe 4T1m/ITIC-4CI/PFH has the ability to target homologous tumors and enhance near-infrared-Ⅱ fluorescence imaging/ultrasound imaging and photothermal therapy effects.
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BACKGROUND:Myocardial infarction is one of the most serious cardiovascular diseases at present,and the existing clinical treatment options such as thrombolytic therapy,percutaneous coronary intervention and coronary artery bypass grafting cannot fully restore the myocardial damage caused by ischemia.Stem cell-derived exosomes for the treatment of myocardial infarction have been a hot research topic in recent years,but the low yield of natural-derived exosomes,the difficulty and time consuming nature of obtaining them,and the poor homing effect have limited their clinical application.In this context,the construction of artificial exosomes as an alternative to natural exosomes has become an effective strategy to solve the above problems. OBJECTIVE:To expound the research status of artificial exosomes in the treatment of myocardial infarction,and classify them into two design modes:semi-artificial and full-artificial,and discuss the research progress and problems of the two modes,finally,make the evaluation and prospect of its clinical application in the future. METHODS:PubMed and CNKI were searched for relevant articles with"artificial exosomes,myocardial infarction,engineering"in Chinese,and"artificial exosome,hybrid exosome,myocardial infarction,nanoparticle,drug delivery system"in English.The focus of the search was from January 2017 to December 2022,and some of the classic forward literature was included.A preliminary selection was conducted through reading titles and abstracts.Repetitive studies,low-quality journals and irrelevant articles were excluded.Finally,73 articles were included for review. RESULTS AND CONCLUSION:(1)By semi-artificially modifying exosomes,whether it is the modification of targeting peptides,hybridization of biofilms or the assistance of magnetic substances,traditional exosome therapies with insufficient targeting and low retention rate and easy to be cleared by the reticuloendothelial system have improved the efficiency of traditional exosome therapy for myocardial infarction.However,these strategies have problems such as unclear modification efficiency,medical ethics,and biotoxicity.(2)Fully artificial bionic exosomes have a higher degree of design freedom compared to exosome modification,which can solve the problems of high extraction and storage difficulties of exosomes of natural origin and limitations of large-scale production;however,this artificial exosome strategy still lacks reliable preclinical data support and biosafety testing,and has not yet formed a standardized process required for large-scale production;therefore,before applying to the clinic,the artificial exosome solution as an alternative to natural exosomes still needs continuous in-depth research by researchers.
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Abstract Background It is justified by the high population's morbidity and mortality rate, as well as the increasing present use of nanoparticles in this pathological context. Objectives To describe the main nanotechnology breakthroughs in the field of cardiovascular disease (CVD) and disseminate pertinent information in the literature. Methods This is a systematic review conducted between September and October 2021. The review was carried out through basic nature, following the initial script for the selective reading of articles in chronological order to collect relevant and consistent data related to the theme. Results It is evidenced the main advances of nanotechnology in the field of CVDs, namely, acute coronary syndromes (ACSs), heart failure (HF), and systemic arterial hypertension (SAH). Conclusion The importance of further and deeper studies in this area is emphasized, in order to make the already approved treatments feasible, so they can reach all publics at a low cost.
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Abstract Objective The present in vitro study incorporated niobium oxyhydroxide fillers into an experimental high-viscosity bulk-fill resin composite to improve its mechanical performance and provide it a bioactive potential. Methodology Scanning electron microscopy synthesized and characterized 0.5% niobium oxyhydroxide fillers, demonstrating a homogeneous morphology that represented a reinforcement for the feature. Fillers were weighed, gradually added to the experimental resin composite, and homogenized for one minute, forming three groups: BF (experimental high-viscosity bulk-fill resin composite; control), BF0.5 (experimental high-viscosity bulk-fill resin composite modified with 0.5% niobium oxyhydroxide fillers), and BFC (commercial bulk-fill resin composite Beautifil Bulk U, Shofu; positive control). In total, 10 specimens/groups (8 × 2 × 2 mm) underwent flexural strength (FS) tests in a universal testing machine (Instron) (500N). Resin composites were also assessed for Knoop hardness (KH), depth of cure (DoC), degree of conversion (DC), elastic modulus (E), and degree of color change (ΔE). The bioactive potential of the developed resin composite was evaluated after immersing the specimens into a simulated body fluid in vitro solution and assessing them using a Fourier-transformed infrared spectroscope with an attenuated total reflectance accessory. One-way ANOVA, followed by the Tukey's test (p<0.05), determined FS, DC, KH, and ΔE. For DoC, ANOVA was performed, which demonstrated no significant difference between groups (p<0.05). Conclusions The high-viscosity bulk-fill resin composite with 0.5% niobium oxyhydroxide fillers showed promising outcomes as reinforcement agents and performed well for bioactive potential, although less predictable than the commercial resin composite with Giomer technology.
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Multi-walled carbon nanotubes (MWCNTs) were purified and unpurified in this study to obtain hybrid materials with improved activity. The production stage comprised a chemical purification of the produced sample. Raman spectroscopy analyzed the structural composition of purified and unpurified samples. The disc diffusion assay, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assessment analyzed antimicrobial activity. MWCNT performed well against the tested bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter aerogenes, Staphylococcus aureus, and Streptococcus sp. Staphylococcus epidermidis). The disc diffusion assay revealed inhibition zone differences caused by purified and unpurified MWCNTs. MIC and MBC values of purified and unpurified MWCNTs were similar. The purified and unpurified nanotubes of Staphylococcus epidermidis (ATCC 35985) exhibited inhibition zone diameters of approximately 8 mm and 9 mm, respectively. The microdilution method revealed a MIC of 1.23 mg/ml for the purified nanotube and 0.156 mg/ml for the unpurified nanotube against the same microorganism. The killing curve analysis demonstrated that unpurified carbon nanotubes were more effective against all tested microorganisms. MWCNTs represent a promising method for microbiology, but studies on the toxicity of these materials remain scarce.
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This comprehensive review paper, titled "Revolutionizing Agriculture: A Comprehensive Review of Nanotechnology Applications," explores the transformative role of nanotechnology in modern agriculture. In a world grappling with food security and environmental challenges, nanotechnology offers innovative solutions that significantly impact the agriculture sector. This paper delves into key topics, including the fundamentals of nanotechnology, various nanomaterials and their applications in agriculture, crop improvement, soil management, pest and disease control, environmental and safety considerations, and the future prospects of nanotechnology in agriculture. The review underscores the importance of nanotechnology in addressing global agricultural challenges, enhancing crop productivity, and ensuring sustainable food production while navigating environmental constraints. By examining the latest research and real-world examples, this review provides valuable insights into the burgeoning field of nanotechnology applications in agriculture, highlighting the significance of interdisciplinary research and responsible nanomaterial utilization.
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Nanotechnology, a field of science and engineering focused on manipulating matter at the nanoscale, has garnered significant attention for its transformative potential across various industries. At the nanometer level, materials exhibit unique properties and behaviors, making it possible to engineer novel solutions with precision and efficiency. Nanotechnology finds applications in diverse areas, from medicine and electronics to energy and environmental remediation. Its ability to create nanoscale structures, devices, and materials has led to groundbreaking innovations, such as drug delivery systems for targeted therapies, ultra-efficient solar cells, and advanced water purification technologies. However, the rapid development of nanotechnology also raises important ethical, safety, and regulatory considerations that require careful attention. As nanotechnology continues to advance, it promises to reshape industries, enhance scientific understanding, and address some of the world's most pressing challenges.
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To safeguard soil, water, and air throughout intensive agricultural operations as well as significant industrial and transportation endeavors, it is imperative that the environment and agriculture are managed sustainably. Application of biochar might be a potential approach to solve these issues. The use of biochar (BC) in agricultural techniques and for environmental remediation has shown to offer a variety of benefits, despite certain drawbacks. Superior physicochemical characteristics of nanobiochar include strong catalytic activity, distinctive nanostructure, large specific surface area, and excellent mobility in soil environments. Nanobiochar is a prime contender for sustainable agriculture to pollution remediation and catalytic reactions. Despite growing interest in biochar research for agricultural and environmental uses, it is unclear how important nanobiochar is. So, in this study, we identified several fundamental uses of nanobiochar with an emphasis on its efficacy for environmentally and agriculturally sustainable practices.
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The science of controlling materials at the nanoscale is known as nanotechnology. Working with the smallest particles conceivable gives rise to aspirations for increasing agricultural production by running into issues that can't be solved traditionally. In the management aspects, attempts are made to restore soil fertility by releasing fixed nutrients, as well as to boost the efficacy of applied fertilizer with the aid of nano clays and zeolites. With the aid of nano biosensors and satellite systems, the input requirements for crops are determined in controlled environments for agriculture and precision farming based on needs, and the necessary quantities are given at the appropriate times and locations. To combat the issues of managing perennial weeds and depleting the weed seed bank, nano herbicides are being developed. By using targeted delivery, slow/controlled release, and conditional release mechanisms, nanostructured formulations could more accurately time the release of their active ingredients in response to biological needs and environmental cues. According to studies, using nano fertilizers improves the efficiency with which nutrients are used, lessens soil toxicity, reduces the frequency of treatment, and minimizes any potential side effects from overdosing. Because of this, nanotechnology, especially in underdeveloped nations, has tremendous potential for attaining sustainable agriculture. To increase agricultural productivity, new technologies are frequently used in agriculture. Employing nanoparticles in agriculture is known as "nanoagriculture," and these particles will have positive effects on crops.
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Agriculture, as one of the oldest and most essential human endeavors, has constantly evolved through the integration of technology. In recent years, nanotechnology has emerged as a pivotal tool, redefining traditional agricultural paradigms. This comprehensive review delves into the multifaceted implications and applications of nanotechnology within agriculture, providing a holistic view of its past, present, and future roles. Historically, nanotechnology's initial foray into agriculture sought to tackle prevalent challenges, from pest control to soil fertility. Despite some early obstacles, this merger has since showcased myriad successful applications, underscored by targeted and efficient solutions that significantly enhance crop yield and food quality. The present-day agricultural landscape is punctuated by nano-fertilizers ensuring optimal nutrient uptake, nanopesticides targeting pests with minimal off-target effects, nanosensors enabling precision agriculture, nano-based food packaging enhancing shelf life, and nanomaterials aiding in disease diagnosis and treatment. However, with innovation come challenges. The environmental and health ramifications of introducing nanoparticles into ecosystems remain a concern. While they promise reduced chemical usage and waste, potential issues like nanoparticle accumulation, unknown long-term effects, and possible toxicity necessitate rigorous research and regulation. Economically, the nano-agri sector promises substantial yield increases, but it also requires significant investments. As the technology permeates the agricultural supply chain, ramifications on job markets, trade dynamics, and global competitiveness become evident. Looking forward, anticipated advancements include smart nanodevices, potent nano-bio interfaces, and self-repairing materials. Nanobots, soil health rejuvenation techniques, and advanced nano-encapsulation are among the many potential R&D avenues. The road ahead requires collaborative efforts from governments, research institutions, farmers, and the private sector. Public-private partnerships, in particular, could prove indispensable, merging public sector oversight with private sector innovation.
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This study investigates farmers' and traders' perceptions of nano urea in the Gondia district of Maharashtra. By providing accurate and long-lasting nutrient management, nano urea, a fertilizer based on nanotechnology, has the potential to revolutionize agriculture. Nearly 120 farmers and 50 merchants were polled as part of the study utilizing a multistage random sampling method. The findings showed that most farmers use nano urea in their fields and are aware of it. The adoption of nano urea by farmers was found to be highly correlated with education. Dealers were well aware of nano urea and eager to sell it since they recognized its benefits in terms of compatibility, storage capacity, and ease of use. However, some farmers voiced their displeasure with lesser paddy field yields and comprehension challenges. The paper discusses the difficulties in implementing and using nano urea in agriculture while highlighting its promise.
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Este artículo pretende alertar acerca de la relevancia de las nanociencias y la nanotecnología acompañada por la nanometrología para el desarrollo de las naciones en el siglo XXI. Se utilizó SciElo como base de datos, con las palabras clave: nanotecnología, nanomedicina, unidades de salud cubanas. El artículo realiza una aproximación a la nanotecnología con enfoque internacional y regional. Describe algunas aplicaciones de las nanotecnologías, con énfasis en la nanomedicina, así como los dilemas y consideraciones éticas asociadas a estas. Advierte acerca de la nocividad para la salud del hombre de algunos de estos desarrollos. Defiende la idea de que la nanometrología, resulta imprescindible para el logro de desarrollos tecnológicos, con mayor relevancia en el campo de la nanomedicina, así como que la nanotecnología debe ser tratada como la ciencia del siglo XXI por el impacto social, cultural y económico que tendrá, y con esto puede cambiar al mundo.
This article aims to alert about the relevance of nanosciences and nanotechnology accompanied by nanometrology for the nations development in the 21st century. Scielo was used as a database, with the keywords: nanotechnology, nanomedicine, Cuban health units. The article makes an approach to nanotechnology with an international and regional approach. It describes some applications of nanotechnologies, with an emphasis on nanomedicine, as well as the dilemmas and ethical considerations associated with them. It advises about these developments' harmfulness to human health, also defends the idea that nanometrology is essential for the technological developments achievement, with greater relevance in the nanomedicine field, as well as that nanotechnology should be treated as the 21st century science due to the social, cultural and economic impact that will have, and this can change the world.
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The field experiment was conducted at Seed Technology Research Center, Department of Plant breeding and Genetics, JNKVV-Jabalpur Kharif-2021. Growth analytical attributes studies indicated superior performance of seed treatment with nano-ZnO @500mg L-1 for LAI @60 DAS (days after sowing), @70 DAS, @80 DAS, LAD, CGR, RGR and BMD w.r.t. 24.26%, 21.39%, 28.50%, 12.09%, 21.42%, 20.17% and 20.56% enhancement over control. Varietal studies revealed superior performance of variety JS 20-116 for LAI, LAD, CGR, RGR and BMD. Biophysical parameters studies revealed superior performance of seed treatment with nano –ZnO @500mg L-1 for chlorophyll content index and energy interception with 5.19% and 26.51% enhancement over control respectively. With respect to variety superior performance of variety JS-20-116 for chlorophyll content index and energy interception. Seed quality parameters studies indicated superior performance of seed treatment with nano–ZnO @500mg L-1 for final plant stand, germination, and seed vigour index I and seed vigour index II with respect to 29.16%, 14.11%, 26.40% and 21.43% enhancement over control. With respect to variety, superior performance of variety JS-20-116 for final plant stand, and seed vigour index I and seed vigour index II and JS 20-34 for seed germination. This results justifies that the seed treatment with nano formulation in the form of ZnO @ 500mg L-1 will improve growth analytical attributes, physiological efficiency and seed quality of the soybean. Hence the seed treatment with nano-ZnO @500mg L-1 is recommended to farmers for maximum productivity, physiological efficiency and sowing seed quality.
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The field of nanotechnology is quickly developing and emerging since it has enormous potential for various human uses. With their unique, size-based physical and chemical attributes, nonmaterials generated and engineered through nanotechnology have many applications in biomedicine and agriculture. Theranostics is a branch of nanomedicine that uses small, highly surface-area nanoparticles to diagnose and treat diseases. Nanomaterials have a wide range of uses in agriculture, from fertilizers that improve soil nutrient uptake by plants to nano pesticides that control a variety of pests, including fungi, phytophagous insects, and weeds, increasing food output. Agriculture and food security are intimately connected, and many researchers are interested. Agriculture production depends on many difficulties due to the severe effects of climate change, water problems and changing the distribution of insect pests. Meanwhile, it faces tremendous challenges in maintaining food security for a massive population amid declining resources. Research is actively exploring the use of nonmaterial in agriculture due to their tremendous promise. Despite their good qualities, nonmaterial present risks to the environment and human health, necessitating risk assessment studies. Green nonmaterial synthesis may lessen the usage of toxic agrochemicals that pollute the environment and enter biological systems, providing an environmentally safe, environmentally friendly, and economically advantageous option. In this critical evaluation, nanoparticles are used in agriculture.
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While the world's population is growing exponentially, there is an alarming increase in the need for food, which might reach a record nine billion people by 2050. In order to address the issue of rising food demand, a number of initiatives are being put into place to boost crop output and safeguard them against agricultural pests. The growing population pressures agricultural civilization to develop fresh strategies for boosting crop output. For nations all across the world, the issue of poverty and malnutrition has grown to be of great concern. Progress in the agricultural sector, which provides the raw materials for the food and feed industries, is crucial for both economic development and population expansion. With economic development, the soil nutrient balances are differed. Soil fertility is important in developing countries for assisting the economy and agriculture. There is a high demand in the twenty-first century for efficient, reliable, and cost-effective systems for detecting, supplying, monitoring, and diagnosing biological host molecules and nutrients. Traditional farming methods are incapable of keeping up with the rate at which food needs are increasing, so we must rely on and incorporate nanotechnology in agriculture and related sectors. In modern agriculture, increasing agricultural productivity is impossible without the use of chemical fertilizers and pesticides; however, the majority of agrochemicals are not eco-friendly and are thus harmful to human health. Nanotechnology is a novel technique for improved and sustainable agricultural production and also harbours a good capacity to bring novel alterations in the agricultural systems. Nanotechnology introduces new technologies and materials for use in molecular biology for the identification of plant pathogenic microorganisms. By bringing novel methods for distributing nutrients through nano fertilizers, specialized pathogen-targeted treatment, and boosting plant pest-resistance, nanotechnology has the potential to change the agricultural industry. Moreover, it can increase plants' ability to absorb nutrients and to endure environmental stresses. The fertility of the soil is a key factor in helping the economy and agriculture in developing nations. The benefits of strategies using nanotechnology for sustainable agriculture are covered here.
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Abstract To assess the in vitro and in situ effect of experimental combined fluoride and calcium nanocomposite solutions on dental caries prevention. Nanocompound mesoporous silica (MS) with calcium (Ca) and sodium fluoride (NaF) - (MSCaNaF); MS with NaF (MSNaF), NaF solution (positive control), and deionized water (negative control - CG) were studied. The specimens (n=130) were submitted in vitro to a multispecies biofilm in the presence of 2% sucrose. After 24 h and 48 h, the culture medium pH, the percent of surface mineral loss (%SML), and lesion depth (ΔZ) were analyzed. In the in situ study, 10 volunteers participated in four phases of 7-days each. The products were applied on the specimens (n=240) before 20% sucrose solution drips. The polysaccharides (SEPS and IEPS), %SML and roughness (Sa) were evaluated. There was an in vitro decrease in pH values in 24h and 48h, compared to baseline. The MSCaNaF and MSNaF groups obtained lower values of %SML and ΔZ (p < 0.05) than CG and NaF after 24h and were similar to NaF after 48h (p<0.05). In situ results showed similar SEPS and IEPS among all groups after 48h. An after 7-days, the nanocomposites had similar values (p>0.05), while NaF was similar to CG (p>0.05). After 48h, the MSCaNaF and MSNaF reduced the %SML (p<0.05). After 7-days, both experimental nanocomposites were similar to NaF (p>0.05). Regarding Sa, MSCaNaF was better than NaF for both periods (p<0.05). The nanocomposites controlled the in vitro and in situ enamel demineralization, mainly in the initial periods.