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Aim To investigate the effect of quercetin (Que) on secretory otitis media (SOM) rats and its mechanism. Methods Forty-eight male SD rats were selected and randomly divided into control group (control), model group (model), Que group (100 mg • kg
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Objective: To investigate the protective effect of emodin on the focal cerebral ischemia in the rats∗ and to clarify its mechanism. Methods: Sixty healthy male SI) rats were randomly divided into sham operation group (given saline), model group (given saline∗ modeling), positive control group (give 5 mg • kg dexamethasone, modeling), low, medium and high doses of emodin groups (given 10, 20, and 40 mg • kg emodin, modeling) (n=10). The middle cerebral artery occlusion (MCAO) was used to establish the rat models of focal cerebral ischemia. The behavioral scores of the rats in various groups were detected ? the percentages of cerebral infarction volumes of the rats in various groups were determined by TTC staining, the levels of tumor necrosis factor (TNF-a)» interleukin-1 beta (IL-lp) » and interleukin-6 (IL-6) in hippocampus tissue at ischemic side of the rats in various groups were measured by enzyme linked immunosorbent assay (ELISA) method' and the expression levels of nuclear transcription factor-icB (NF-kB) p65 protein and nuclear transcription factor kB inhibitor-a (bcBa) protein in hippocampus tissue at ischemic side of the rats in various groups were detected by Western blotting method. Results: No neurological defects or cerebral infarction of the rats in sham operation group was found. Compared with sham operation group∗ the levels of IL-6∗ IL-1|3∗ and TNF-a in hippocampus tissue of the rats in model group were significantly increased (P'<0. 05) ∗ while the expression level of NF-kB p65 protein was significantly increased ( P<∗0. 05)∗ and the expression level of IkBo protein was decreased significantly (P-<0. 05). Compared with model group∗ the behavioral scores of the rats in positive control group∗ medium and high doses of emodin groups were significantly decreased ( P<0. 05)∗ the percentages of cerebral infarction volumes were decreased ( P<∗0. 05)∗ the levels of IL-6∗ IL-lp∗ and TNF-a in hippocampus tissue at ischemic side of the rats were significantly decreased (P<0.05)∗ the expression levels of NF-kB p65 protein were significantly decreased ( P
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Objective To investigate the effect of transcatheter arterial chemoembolization combined with radiofrequency ablation in the treatment of primary hepatocellular carcinoma (HCC) and its influence on the levels of nuclear factor kappa B (NF-B) and p53.Methods 90 patients with primary HCC were selected,and they were randomly divided into control group (n =45) and observation group (n =45) according to the digital table.The control group received chemoembolization treatment,the observation group was given combined radiofrequency ablation treatment.The patients were continuously treated for 6 months.Immunohistochemical assay was used to detect the expression of NF-B and p53 in two groups.The clinical curative effect and influence on the expression of NF-B and p53 were compared between the two groups.Results After 6 months of treatment,the Karnofsky score of the observation group was (84.32 ± 12.31)points,which was higher than (64.32 ± 11.24) points of the control group (t =21.295,P ＜ 0.05).After treatment for 6 months,the AFP level of the observation group was (121.23 ± 1.43) μg/L,which was lower than (189.44 ± 36.42) μg/L of the control group (t =19.853,P ＜ 0.05).After treatment,the positive expression rates of NF-B and p53 of the observation group were 24.44％ and 20.00％,respectively,which were lower than 40％ and 33.33 ％ of the control group (x2 =6.597,5.784,all P ＜ 0.05).The recurrence rate of the observation group was 15.56％,which was lower than 28.89％ of the control group (x2 =6.126,P ＜ 0.05).The 6-month and 12-month survival rates of the observation group were 86.67％ and 82.22％,respectively,which were higher than 75.56％ and 64.44％ of the control group (x2 =4.927,7.192％,all P＜0.05).Conclusion The effect of radiofrequency ablation combined with radiofrequency ablation for patients with primary liver cancer is satisfactory,which is worthy of popularization and application.
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Background Free-flap Epi-LASIK surgery show a fast epithelial healing and mild trauma responses.Its mechanism is notable. Objective The aim of this study was to compare the corneal epithelial healing response following photorefractive keratectomy(PRK)and flap-free Epi-LASIK surgery. Methods Twelve experimental rabbits were randomly assigned to high cutting group(-10.00 D)and low cutting group(-3.00 D),and 2 normal matched rabbits were used as controls.PRK was performed in lateral eyes and flap-free epipolis laser in situkeratomileusis(Epi-LASIK)surgery was curried out in the fellow eyes.The corneal wound healing response in early stage of post-operation was examined under the slim lamp,and the inflammatory cells infiltration in cornea,basic fibroblast growth factor(bFGF)and nuclear transcription factor(NF-KB)expressions in corneal stroma were detected by hematine-eosin staining and immunohistochemstry in the seventh day after operation. Results The inflammatory reaction was milder in the eyes with Epi-LASIK than ones of PRK under the slim lamp.No significant difierences were found in numbers of inflammatory cells in cornea.expressions of bFGF and NF-κB in corneal stroma between two types of surgeries in low cutting group(P＞0.05).In high cutting group,the numbers of inflammatory cells were(12.25±1.22)/400 field in PRK eyes,and those of free-flap Epi-LASIK eyes were(6.67±0.31)/400 field,showing obvious difference between them(t=-8.87,P＜0.01).Expressions of NF-κB(A value)in cornea were 6.11±1.36 and 41.82±8.7 1 in PRK eyes of low cutting group and high cutting group.and those in free-flap Epi-LASIK were 3.01±0.81 and 11.59±4.55.indicating a drastically increase in PRK eyes(P＜0.05).The expressions of bFGF(A value)in corneal stroma were 33.59±6.98 and 123.68±18.81 in PRK eyes and those in free-flap Epi-LASIK eyes were 69.20±8.85 and 123.68±1 8.81 in low cutting group and high cutting group,presenting a considerably decrease in PRK eyes (P＜0.05).The corneal epithelial healing was faster in free-flap Epi-LASIK eyes than that in PRK eyes.and deeper cutting slowed the healing procedure. Conclusion In low cutting group,healing time of corneal epithelium is close between PRK and flap-free Epi-LASIK.But in high cutting group,epithelial healing is faster and inflammatory reaction is lighter in flap-free Epi-LASIK compared with PRK.