RESUMEN
Objective To study the mechanism of 308 nm excimer laser combined with piperine activated the production of melanin in amelanotic melanocytes in outer root sheath of hair(AMMC) ,and promote the expression of TRP‐1、TRP‐2 protein . Methods AMMC were in incubated for 24、72、120 h in different groups(combination therapy group ,the 308 nm excimer laser group ,the piperine group ,the positive control group and the control group) .Then the melanin content was measured .The expres‐sion change of TRP‐1 and TRP‐2 in cells were observed by laser scanning confocal microscopy .Results Four groups increased the AMMC cellular melanin content .Four different groups could promote melanin content at different degrees .The combination therapy group played the strongest role in promoting function .And the function was the most obviously in 24 h ,it also was concentration dependent .0 .5 mmol/L piperine in combination group and piperine group could produce promoting effect of within 72 h on the ex‐pression of TRP‐1 ,but its role was not evident in the expression of TRP‐2 .308 nm excimer laser had effects both on TRP 1 and TRP 2 .Conclusion 308 nm excimer laser combination with piperine can increase the activation of amelanotic melanocytes in outer root sheath of hair .