RESUMEN
Objective: To evaluate the toxicological safety of over-ground parts of Glycyrrhiza uralensis, in order to provide basis for the rational utilization of over-ground parts of Glycyrrhizae Radix et Rhizoma recourses.Method: Mice acute oral toxicity test, micronucleus test of mice bone marrow, mice sperm shape abnormality test and toxicological test based on chronic nonbacterial prostatitis model were carried out.Result: Maximal tolerable dose(MTD) of over-ground parts of G.uralensis water extract (WE) and alcohol extract (AE) were 96,128 g·kg-1, respectively. Macro-porous resin enriched product of AE was harmful to mice, with gender differences. Micronucleus rates of each dose(8,16,32 g·kg-1) group and control group for female mouse were 0.28%, 0.34%, 0.26% and 0.22%, respectively. Micronucleus rates of each dose(8,16,32 g·kg-1) group and control group for male mouse were 0.32%, 0.30%, 0.36% and 0.28%, respectively. Sperm shape abnormality rates of each dose group and control group were 3.16%, 3.01%, 2.67% and 3.23%, respectively. Micronucleus rate and sperm shape abnormality rate had no significant increase compared with the negative control. The 30-day repeated intragastric WE and AE had no effect on the general conditions of the model rats. Compared with normal group, AE group showed a significant decrease in heart weight, and significant increases in liver weight, liver index and kidney index (PPConclusion: The results indicated that both of WE and AE have potential toxicity. WE does not show any genetic toxicity to mice. Therefore, further studies shall be made for toxicological safety of over-ground parts of G. uralensis.
RESUMEN
Introduction: Gait evaluation and training using treadmill will be increasingly used in near future. However it is con¬troversial whether the treadmill replicates the overground environment. Goal: Aim of this study was to compare overground and treadmill ambulation for possible differences in gait tempo¬ral variables and leg joint kinematics. Materials and Methods: A total of 10 participants walked on overground and treadmill. Participants walked at their preferred velocity on overground. The treadmill velocity was adjusted average velocity obtained in overground walking. Walking in two conditions was captured by high speed camera and analyzed by motion analyses software. Results: The maximum hip flexion angle (P=0.046), maximum hip extension (P=0.0001), maximum knee extension (P=0.0001) and maximum ankle dorsiflexion (P=0.022) were significantly different in the two conditions. Conclusions: The present study suggest that statistically significant differences exist between overground and treadmill walking in healthy subjects for some joint kinematic and temporal variables.