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Aim: The aim of this study was identify contamination on absorbent paper points used by students of Dental Clinic III of the Faculty of Dentistry of Federal University of Rio Grande do Sul in the semester 2015/1, in order to warn students and professionals of the area on the importance to sterilize these materials. Material and Methods: In a clinical environment, 180 absorbent paper points we collected (80 of them from the first series and 80 from the second one), from 40 students. After the collection, each one was singly immersed in a microtube containing 1.5 ml of the BHI (Brain Heart Infusion) culture. Positive control was composed by one paper point contaminated by saliva, and negative control was composed by a closed microtube, only with BHI. The microtubes were incubated at 37ºC in bacteriological incubator during 14 days. The microtubes that presented turbidity were considered positive, and those which did not present turbidity were considered negative. Results: The results were analyzed by the Fisher Exact Test, which demonstrated that paper points from the second series presented higher agreement contamination between the paper points collected from each box, when compared to the analyzed boxes from the first series (p=0.03). All the samples observed presented growth of Bacillus spp in the microorganism identification. Conclusions: It is possible conclude that absorbing paper points, when exposed to clinical environment suffer contamination, and the autoclave sterilization is necessary before the use, regardless the commercial brand, in order to ensure the aseptic chain maintenance.
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Objective To establish and evaluate the method to collect the rat gingival crevicular fluid (GCF)by using absorbent paper points, and to lay foundation for analysis on GCF.Methods 20 healthy male rats were selected and randomly divided into GCF group and saliva group.The GCF of the right upper molar gingival trough of the rats in GCF group and the saliva of the rats in saliva group were collected by using 1 5# absorbent paper points.The SDS-PAGE analysis and abundance detection were applied to analyze the protein bands of the samples in two groups.Results The SDS-PAGE analysis identified the proteins at 77 000,66 000,55 000,51 000,and 28 000,especially 66 000 in GCF group.While saliva group had lower brightness protein bands at 66 000,60 000, and 48 000.The data of protein abundance of 66 000 between two groups had statistically significant difference (P<0.05).Conclusion The number and types of the protein bands are different between GCF Group and saliva group,so using 15# absorbent paper points can collect the rat GCF successfully.
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Objetivo: Avaliar a influência do processo de esterilização sobre cones de papel em relação à sua capacidade de absorção e, conseqüentemente,de secagem dos condutos radiculares, além da possível liberação de algum produto antimicrobiano ou citotóxico. Métodos: Os cones utilizados foram de três marcas encontradas no mercado brasileiro: Dentsply (Dentsply Indústria e Comércio Ltda., Petrópolis,Brasil), Endopoints (Endopoints Indústria e Comércio Ltda., Paraíba do Sul, Brasil) e Tanari (Tanari Industrial Ltda., São Paulo, Brasil). Para avaliação da capacidade de absorção, os cones foram submetidos a quatro ciclos de esterilização e foi realizada a técnica de Holland modificada. A capacidade antimicrobiana/citotóxica foi verificada por meio do depósito dos cones esterilizados em placas de Petri contendo Ágar Miller-Hintom e Ágar Sangue, semeados com S.aureus e E. coli. Resultados: Os cones Dentsply (Dentsply Indústria e Comércio Ltda., Petrópolis, Brasil) e Tanari (Tanari Industrial Ltda., São Paulo, Brasil) apresentaram maior absorção após o primeiro ciclo de esterilização, seguido de queda no segundo e terceiro ciclo e novo aumento no quarto ciclo. Para os cones Endopoints (Endopoints Indústria e Comércio Ltda., Paraíba do Sul, Brasil), os valores foram inversos, com pequena queda de absorção após o primeiro ciclo, aumento no segundo e terceiro e nova queda no quarto ciclo. Nenhum dos cones apresentou atividade antimicrobiana após o processo de esterilização. Conclusão: O processo de esterilização por calor úmido não altera as propriedades de absorção e não há liberação de subprodutos dos cones de papel testados.
Objective: To evaluate the influence of the sterilization process on paper cones as regards their absorption capacity, and consequently, root canal drying, in addition to the possible release of any antimicrobial or cytotoxic product. Methods: The cones used were of three of the brands found on the Brazilian market Dentsply (Dentsply Indústria e Comércio Ltda., Petrópolis, Brazil), Endopoints (Endopoints Indústria e Comércio Ltda., Paraíba do Sul, Brazil) and Tanari (Tanari Industrial Ltda., São Paulo, Brazil). To evaluate the absorption capacity, the cones were submitted to four sterilization cycles, and the modified Holland technique was performed. The antimicrobial/cytotoxic capacity was verified by means of depositing the sterilized cones in Petri dishes containing Miller-Hinton Agar and Blood Agar, seeded with S.aureus and E. coli. Results: The Dentsply (Dentsply Indústria e Comércio Ltda., Petrópolis, Brazil) and Tanari (Tanari Industrial Ltda., São Paulo, Brazil) cones presented greater absorption after the first sterilization cycle, followed by a drop in the second and third cycles, and a new increase in the fourth cycle. For the Endopoints (Endopoints Indústria e Comércio Ltda., Paraíba do Sul, Brazil) cones, the values were inverted, with a small drop in absorption after the first cycle, increase in the second and third cycles, and a new drop in the fourth cycle. None of the cones presented antimicrobial activity after the sterilization process. Conclusion: The sterilization process by damp heat does not alter the properties of absorption and there is no release of by-products from the tested paper cones.