Flavor and meridian tropism classification analysis of Callianthemum taipaicum / 中国中药杂志

To explore the flavor and meridian tropism classification of Callianthemum taipaicum by principal components analysis(PCA) and partial least square analysis(PLS). Meanwhile,to establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-ESI-MS) method for the simultaneous determination of 55 active components from 13 kinds of Ranunculaceae of Chinese traditional herbs. Samples were separated on HPLC system by Agilent 5 TC-C₁₈（2）（4.6 mm×250 mm，5 μm）column and eluted with acetonitrile and 0.1% formic acid at the flow rate of 0.6 mL·min⁻¹. The data were performed by HPLC-ESI-MS with multiple reaction monitoring（MRM）scanning mode under positive and negative ion modes and quantified by external standards. The data from 13 Ranunculaceae herbs were analyzed by the PLS-tree and cooman's prediction of PCA and PLS to evaluate the similarities and differences of C. taipaicum in flavor and meridian tropism. The results showed that calibration curves of 55 components all showed good linearity, >0.99,with good precision, repeatability and stability. After compared to other 12 herbs,PCA and PLS results revealed that the C. taipaicum belonged to lung and bladder meridians while its flavor attributive to pungent,warm in nature. In conclusion,the analysis approach of chemometric calculation combined with multi-components quantification is suitable for the classification of meridian tropism and flavor of Chinese traditional medicine,which can be used for alternative research of rare herbs.

Based on ~1H-NMR-PR to establish an identification method of Ophiopogonis japonicus from different habitats / 中草药

Objective To establish a new identification method of Ophiopogonis japonicus from different habitats.Methods Using 1H-NMR to get the all component information on O.japonicus,and using pattern recognitions,such as principal component analysis(PCA),partial least squares-discriiminate analysis(PLS-DA),and hierarchical cluster analysis(HCA) to analyze the data from the 1H-NMR spectra.Results The 1H-NMR-pattern recognition(PR) method could identify the samples of O.japonicus from different habitats successively.Conclusion The 1H-NMR-PR is an useful method for identification of O.japonicus from different habitats and could be used for the quality control of traditional Chinese medicinal materials.