RESUMEN
AIM To study the effects of A 3 site (components), extracted from Angelica naphtha (total oil),on physiological properties of isolated rat atrium and action potential of isolated guinea pig ventricular papillary muscles. METHODS Spontaneous beats, contractile force and functional refractory period(FRP) of isolated atrial muscle were recorded according to the regular methods. Standard microelectrode intracellular recording technique was used to record action potential(AP) of ventricular papillary muscles. RESULTS A 3 site(10～160 mg?L -1 ) dramatically inhibited spontaneous beats of isolated rat right atrium and made beats stop with 160 mg?L -1 . A 3 site concentration dependently reduced contractile force of isolated rat left atrium induced by electrostimulating, with the IC 50 value of 52 3 mg?L -1 . A 3 site(40～100 mg?L -1 ) obviously prolonged FRP. The FRP was prolonged from 106 ms (as control) to 130 ms at final concentration of 100 mg?L -1 . A 3 site decreased action potential amplitude (APA), shortened 20%APD(APD 20 ) and 90%APD(APD 90 ), but had no significant effect on RP of AP. CONCLUSION The mechanism of these effects of A 3 site on myocardial physiological properties and action potential was probably associated with blocking the influx of Ca 2+ and Na +, promoting K + outflux.
RESUMEN
This study was designed to investigate the properties of the three typical kendo practices (kirikaeshi, kakarigeiko, and jigeiko) in terms of changes in urine flow and creatinine excretion after practice events. The subjects were six male university kendo practitioners. They performed four exercises that were three kendo practices and a warming up without a kendo practice as the control, at a separate day respectively. In each exercise, five urine samples were collected continuously as follows: (1) after 30 minutes rest (rest), (2) immediately after warming up (w-up), (3-5) at every 30 minutes after practice event up to 90 minutes (30'--90'-urine) . Two blood sam-ples were collected at the rest period and 5-10 minutes after exercise. The blood lactate concentration after kendo practices rose to 1.09-8.02 times of rest level. The 30'-urine flow tended to decrease than w-up in kirikaeshi, jigeiko and control, but to increase in kakarigeiko. Creatinine concentration of 30'-urine rose significantly in the control, kirikaeshi and jigeiko than w-up, but significantly fell in kakarigeiko. The kakarigeiko brought peculiar changes in 30'-urine. The urinary changes after exercises (30'-urine rest) indicated nonlinear relation with blood lactate change ratios. These results indicate that the kendo practices bring the two-way influences in urinary change, one is diuretic effect accompanied with lactate accumulation in the kakarigeiko, and the other is the antidiuretic effect accompanied with less lactate accumulation in the kirikaeshi and jigeiko.