RESUMEN
[Objective]To observe the intervention of Electroacupuncture at Point Jianyu(LI15) on Rabbit Model of Scapulohumeral Periarthritis for receiving possible mechanism.[Methods ]Thirty healthy rabbits(male or female) were randomly divided into three groups:scapulohumeral periarthritis model group,EA Jianyu group and Ibuprofen control group.Each group respectively were detected the concentrations of serum PGE2 before model and treatment and after treatment,and then killed them to achieve tendons of shoulder which were detected the concentrations of Hydroxyproline.And achieve tissues around shoulder which were studied by HE to investigate the changes of the histology.[Results]The concentrations of serum PGE2 had no notable difference between EA Jianyu and model group,Ibuprofen control group and model group after treatment.But it was lowered after treatment than before treatment by EA Jianyu.There were no significantly difference among the three groups about concentrations of Hydroxyproline in tendons,but Ibuprofen control group was higher than EA Jianyu group.Tissues of the shoulder were observed that EA Jianyu restrained reaction of inflammation and degenerescence of tissues,and promoted it better.[Conclusions] EA Jianyu has anti-inflammatory effect and cuts back on the degree of degenerescence of tissues on scapulohumeral periarthritis model in rabbit.
RESUMEN
Objective To understand the influence of bradykinin(Bk) on the signaling of prostaglandin E_2(PGE_2) in cultured bovine trabecular meshwork cells(TMCs),and to discuss the mode of interaction between the two signal pathways in TMCs.Methods TMCs were incubated in 1000nmol/L PGE_2 or 1000nmol/L PGE_2 combined with 100nmol/L Bk for 60s.The production of intracellular cAMP was assayed by radioimmunoassay.Results Bk could magnify the stimulation of PGE_2 on cultured TMCs.PGE_2 combined with Bk could cause greater elevation of intracellular cAMP than PGE_2 alone(P