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1.
China Pharmacy ; (12): 186-191, 2024.
Artículo en Chino | WPRIM | ID: wpr-1006176

RESUMEN

OBJECTIVE To investigate the inhibitory effects of Ginkgo biloba extract (GBE) on renal inflammation in diabetic nephropathy (DN) model mice, and its potential mechanism. METHODS KK/Ay mice were fed with high fat and high sugar to induce DN model. They were divided into model group, positive control group [metformin 200 mg/(kg·d)], GBE low-dose and high-dose groups [100, 200 mg/(kg·d)], with 6 mice in each group. Six C57BL/6J mice were fed with a regular diet as the control group. Administration groups were given relevant liquid intragastrically, control group and model group were given constant volume of normal saline intragastrically, once a day, for 8 consecutive weeks. The body weight, fasting blood glucose, 24-hour food intake, 24-hour urine output, monocyte chemoattractant protein-1 (MCP-1), interleukin-12 (IL-12), IL-10, advanced glycation end products (AGEs), blood urea nitrogen (BUN) and serum creatinine (Scr) of mice were measured, and the ratio of bilateral kidneys to body weight was also calculated. The pathological injury and fibrotic changes of the renal cortex were observed, and the expressions of macrophage polarization marker proteins [type M1: inducible nitric oxide synthase (iNOS); type M2: arginase-1 (Arg-1)] and AGEs-the receptor of advanced glycation end products (RAGE)/Ras homolog gene pharm_chenjing@163.com family member A (RhoA)/Rho-associated coiled-coil forming protein kinase (ROCK) signaling pathway-related proteins were determined in renal cortex. RESULTS Compared with the model group, the symptoms such as renal cortical hyperplasia, vacuoles, infiltration of inflammatory cells, and renal cortical fibrosis had been improved in GBE low-dose and high-dose groups; body weight, serum level of IL-10, the expression of Arg-1 in the renal cortex were significantly higher than model group (P< 0.01); fasting blood glucose, 24-hour food intake, 24-hour urine output, serum levels of MCP-1, IL-12, BUN, Scr and AGEs, the ratio of bilateral kidneys to body weight, renal injury score, the proportion of renal interstitial fibrosis, the protein expressions of iNOS, RAGE, RhoA and ROCK1 (except for GBE low-dose group) in renal cortex were significantly lower than model group (P<0.01). CONCLUSIONS GBE could improve kidney damage and alleviate inflammatory response in DN model mice, the mechanism of which may be related to inhibiting the AGEs-RAGE/RhoA/ROCK signaling pathway and regulating macrophage polarization.

2.
Indian J Ophthalmol ; 2023 Jul; 71(7): 2756-2759
Artículo | IMSEAR | ID: sea-225124

RESUMEN

Purpose: Ripasudil is a class of drug which alters the trabecular meshwork to increase the aqueous outflow and has been shown to be effective in pseudoexfoliative glaucoma (PXF G). This study aimed at assessing the efficacy and safety profile of ripasudil as an adjunct treatment in patients with PXF G at maximal tolerated antiglaucoma medications. Methods: In this prospective, interventional study, 40 patients with PXF G were enrolled between May 2021 and Jan 2022. Ripasudil 0.4% was started as an adjunctive drug to the ongoing antiglaucoma medications. On follow?up visits at 1, 3, and 6 months, the visual acuity, intraocular pressure (IOP), anterior segment, and fundus findings were evaluated. The premedication and postmedication IOP values were compared by paired t?test, and a P?value <0.05 was considered statistically significant. Results: Average age at recruitment was 60.02 ± 8.74 years. Baseline premedication IOP was 25.375 ± 3.276 mmHg. IOP reduction at 6 months was found to be statistically significant in all patients, with the maximal response being 24.13%. Also, 87.5% (35/40) of patients reached target IOP or even lower IOP at the end of study. There was no statistically significant association between the PXF grade and IOP. However, the grade of inferior iridocorneal angle pigmentation was found to be higher in eyes with elevated IOP (P < 0.05). Only three patients developed conjunctival hyperemia as an adverse reaction, which was mild and transient. Conclusion: Ripasudil showed additional IOP?lowering effect with other antiglaucoma medications and exhibited no significant side effects

3.
Movimento (Porto Alegre) ; 29: e29002, 2023. graf
Artículo en Inglés | LILACS | ID: biblio-1506742

RESUMEN

This investigation discusses the relationship between the body culture of movement and two rock art scenes located in the Parque Nacional Serra da Capivara, (Serra da Capivara National Park) - PNSC, in the state of Piauí, Brazil. The scenes depict a flic flac and a human pyramid, which have been accepted in contemporaneity as gymnastics and acrobatics. An interdisciplinary theoretical apparatus based on Physical Education and its relationships with Archeology and Anthropology was used in the analysis. The analysis is based on inferences, as nothing guarantees that the hands that portrayed the scenes had the same intentions as the authors of the research. One of the findings understands that motor skills helped the daily demands of adversity for the survival of the group. In another point of view, based on the Ilinx concept, flic flac satisfies the search for a trance/vertigo vital to ritualistic liturgy, and the human pyramid serves the imperative task of consolidating cooperative bonds and trust between its members in a hostile environment. (AU)


A investigação discute as relações entre duas artes rupestres - flic flac e a pirâmide humana -, admitidas na contemporaneidade como ginástica e acrobacia, localizadas no Parque Nacional Serra da Capivara - PNSC, no Estado do Piauí, Brasil, e a cultura corporal do movimento. Utilizou-se de um aparato teórico interdisciplinar fundamentado na Educação Física e suas relações com a Arqueologia e Antropologia na análise. Trata-se de inferências, pois nada garante que as mãos que retrataram as cenas tinham a intencionalidade dos autores da pesquisa. Uma das ilações entende que as habilidades motoras auxiliavam as demandas do cotidiano de adversidades para sobrevivência do grupo. Por outro prisma, a partir do conceito de Ilinx, o flic flac atende a busca de um transe/vertigem vital a liturgia ritualística, e, a pirâmide humana serve a imperiosa tarefa de consolidar os laços cooperativos e de confiança entre os seus membros em um ambiente hostil. (AU)


Esta investigación discute la relación entre la cultura corporal del movimiento y dos escenas de arte rupestre ubicadas en el Parque Nacional Serra da Capivara/PNSC, en el estado de Piauí, Brasil. Las escenas representan flic flac y pirámide humana, que han sido aceptadas en la contemporaneidad como gimnasia y acrobacia. Se utilizó en el análisis un aparato teórico interdisciplinario basado en la Educación Física y sus relaciones con la Arqueología y la Antropología. El análisis se basa en inferencias, nada garantiza que las manos que retrataron las escenas tuvieran las mismas intenciones que los autores de la investigación. Uno de los hallazgos comprende que la motricidad ayudó a las exigencias diarias de la adversidad para la supervivencia del grupo. En otro punto de vista, basado en el concepto Ilinx, flic flac satisface la búsqueda de trance/vértigo vital para la liturgia ritualista, y la pirámide humana cumple la tarea imperativa de consolidar lazos de cooperación y confianza entre sus miembros en un ambiente hostil. (AU)


Asunto(s)
Cultura , Movimiento
4.
Chinese Journal of Dermatology ; (12): 222-228, 2023.
Artículo en Chino | WPRIM | ID: wpr-994465

RESUMEN

Objective:To investigate effects of the ROCK1 gene on proliferation and migration of and related molecular expression in keloid fibroblasts.Methods:Immunohistochemical technique was used to detect ROCK1 protein expression in human keloids and normal skin tissues, and Western blot analysis was performed to detect the expression of ROCK1, transforming growth factor β1 (TGF-β1) and E-cadherin in keloid tissues. In vitro cultured human keloid fibroblasts (HKFs) were divided into 4 groups: ROCK1 gene overexpression control group (ROCK1 NC group) transfected with ROCK1 gene overexpression control vectors, ROCK1 gene overexpression group (ROCK1 OE group) transfected with ROCK1 gene overexpression vectors, ROCK1 gene knockdown control group (sh NC group) transfected with ROCK1 gene knockdown control vectors, and ROCK1 gene knockdown group (shROCK1 group) transfected with ROCK1 gene knockdown vectors. Cell counting kit-8 (CCK8) assay was performed to evaluate the effect of ROCK1 gene on the survival rate of HKFs, Transwell assay to evaluate the effect on the migration of HKFs, and real-time fluorescence-based quantitative PCR (qRT-PCR) and Western blot analysis were conducted to determine the mRNA and protein expression of ROCK1, TGF-β1 and E-cadherin, respectively. Results:Immunohistochemical study showed that ROCK1 protein expression decreased significantly in the human keloid tissues compared with the normal tissues ( t = 6.47, P = 0.003) ; Western blot analysis showed that the expression levels of ROCK1 and E-cadherin significantly decreased ( t = 14.02, 162.20, respectively, both P < 0.001), while TGF-β1 expression significantly increased ( t = 76.01, P < 0.001) in the keloid tissues compared with the expression levels of corresponding proteins in the normal tissues. CCK8 assay showed that the cell activity was significantly lower in the ROCK1 OE group than in the ROCK1 NC group after 24-hour transfection ( t = 3.25, 3.78, P = 0.031, 0.019, respectively), and significantly higher in the shROCK1 group than in the sh NC group ( t = 3.12, 2.79, P = 0.036, 0.049, respectively). Transwell assay showed that the number of migratory cells was significantly lower in the ROCK1 OE group than in the ROCK1 NC group ( t = 5.17, P = 0.004), and significantly higher in the shROCK1 group than in the sh NC group ( t = 9.28, P < 0.001). Compared with the ROCK1 NC group, the ROCK1 OE group showed significantly increased mRNA and protein expression levels of ROCK1 and E-cadherin ( P < 0.05 or < 0.001), but decreased mRNA and protein expression levels of TGF-β1 (both P < 0.001) ; compared with the sh NC group, the shROCK1 group showed significantly decreased mRNA and protein expression levels of ROCK1 and E-cadherin ( P < 0.05 or < 0.001), but significantly increased mRNA and protein expression levels of TGF-β1 ( P = 0.005 or < 0.001) . Conclusions:The ROCK1 gene can inhibit the proliferation and migration of HKFs. Overexpression of the ROCK1 gene can down-regulate the TGF-β1 gene expression and up-regulate the E-cadherin gene expression in HKFs.

5.
Chinese Journal of Clinical Pharmacology and Therapeutics ; (12): 383-390, 2023.
Artículo en Chino | WPRIM | ID: wpr-1014645

RESUMEN

AIM: To investigate the effects of hyperoside on traumatic brain injury (TBI) rats by regulating the Ras homolog gene family, member A (RhoA)/Rho-associated coiled coil-forming kinase (ROCK) signal pathway. METHODS: The TBI rat model was established by modified Feeney free fall hit method, and was randomly divided into model group, low-dose hyperoside (60 mg / kg) group, high-dose hyperoside (120 mg / kg) group, high-dose hyperoside (120 mg / kg) + no load group, and high-dose hyperoside (120 mg / kg) + RhoA overexpression group, with 10 rats in each group, another 10 healthy rats were set as sham operation group, after hyperoside and plasmid were grouped, the nerve injury was detected by modified neurological deficit score (mNSS) and dark avoidance test; Evans blue (EB) quantitative method was used to detect the permeability of blood brain barrier in rats; ultrastructural damage of blood-brain barrier was observed by transmission electron microscopy; the levels of tumor necrosis factor- α (TNF- α), interleukin-8 (IL-8), superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and brain tissue of rats were measured with the kit; and the expression of RhoA/ROCK pathway related proteins in rat brain was detected by Western blot. RESULTS: Compared with the sham operation group, the blood brain barrier structure of the model group rats was damaged, the step-through latency and SOD level decreased obviously (P0.05). CONCLUSION: Hyperoside can inhibit neuroinflammation and oxidative stress in TBI rats by down-regulating RhoA / ROCK signal pathway, thereby reducing the damage of blood brain barrier and repairing its neural function.

6.
Braz. j. biol ; 83: 1-8, 2023. map, tab
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1468864

RESUMEN

The current study was carried out to estimate the prevalence and diversity of ectoparasites in rock pigeons in different regions of Punjab, Pakistan. A total of 120 birds were captured from March 2017 to February 2019. The ectoparasites were collected by standard procedures and preserved in 70% ethanol containing one drop of glycerin. Data related to age, health status, sex, type of area, sampling location and season were collected using a standardized form. Ectoparasites were identified based on morphological characteristics by using identification keys. Ninety-six (80%) birds were infested with ectoparasites. A total of seven families and thirteen species of different ectoparasites were observed. Mainly, seven species of lice, two species of flies, one species of tick and three species of mites were recovered from infested birds. The female pigeons were more often infested (89.02%) than male pigeons (60.52%). The prevalence was found higher during summer (100%) as compared to other seasons. The infestation rate was higher in Industrial area (97.50%) as compared to other regions. The highest prevalence of ectoparasites (100%) was recorded from Sargodha district. There was significant (P < 0.05) variation among number of ectoparasites on wing, chest, tail and neck within age groups, seasons and ecological zones. The occurrence of parasites in relation to area, age, health status, sex and season were significant. The infestation rate of parasites in rock pigeon is high in different districts of Punjab. It is recommended that these wild birds infested with multiple species of ectoparasites could be the potential source of infestations in domesticated birds if they come in contact with them. The contact of domesticated birds should be prevented from wild birds to minimize the chance of cross species transmission of ectoparasites.


O presente estudo foi realizado para estimar a prevalência e diversidade de ectoparasitas em pombos-das-rochas em diferentes regiões de Punjab, Paquistão. Um total de 120 aves foram capturadas de março de 2017 a fevereiro de 2019. Os ectoparasitas foram coletados por procedimentos padrão e preservados em etanol 70% contendo uma gota de glicerina. Os dados relativos à idade, estado de saúde, sexo, tipo de área, local de amostragem e época do ano foram coletados em formulário padronizado. Os ectoparasitas foram identificados com base nas características morfológicas por meio de chaves de identificação. Noventa e seis (80%) aves estavam infestadas com ectoparasitas. Um total de sete famílias e treze espécies de diferentes ectoparasitas foram observados. Principalmente, sete espécies de piolhos, duas espécies de moscas, uma espécie de carrapato e três espécies de ácaros foram recuperadas de aves infestadas. Os pombos fêmeas foram infestados mais frequentemente (89,02%) do que os pombos machos (60,52%). A prevalência encontrada foi maior no verão (100%) em comparação com as outras estações. A taxa de infestação foi maior na área Industrial (97,50%) em relação às demais regiões. A maior prevalência de ectoparasitas (100%) foi registrada no distrito de Sargodha. Houve variação significativa (P <0,05) entre o número de ectoparasitas na asa, tórax, cauda e pescoço dentro das faixas etárias, estações do ano e zonas ecológicas. A ocorrência de parasitas em relação à área, idade, estado de saúde, sexo e estação do ano foi significativa. A taxa de infestação de parasitas em pombo-correio é alta em diferentes distritos de Punjab. Recomenda-se que essas aves selvagens infestadas com várias espécies de ectoparasitas possam ser a fonte potencial de infestações em aves domesticadas se entrarem em contato com elas. O contato de aves domesticadas deve ser evitado com aves selvagens para minimizar a chance de transmissão cruzada de ectoparasitas.


Asunto(s)
Masculino , Femenino , Animales , Garrapatas , Columbidae/parasitología , Dípteros , Phthiraptera , Ácaros , Prevalencia
7.
Braz. j. biol ; 832023.
Artículo en Inglés | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469080

RESUMEN

Abstract The current study was carried out to estimate the prevalence and diversity of ectoparasites in rock pigeons in different regions of Punjab, Pakistan. A total of 120 birds were captured from March 2017 to February 2019. The ectoparasites were collected by standard procedures and preserved in 70% ethanol containing one drop of glycerin. Data related to age, health status, sex, type of area, sampling location and season were collected using a standardized form. Ectoparasites were identified based on morphological characteristics by using identification keys. Ninety-six (80%) birds were infested with ectoparasites. A total of seven families and thirteen species of different ectoparasites were observed. Mainly, seven species of lice, two species of flies, one species of tick and three species of mites were recovered from infested birds. The female pigeons were more often infested (89.02%) than male pigeons (60.52%). The prevalence was found higher during summer (100%) as compared to other seasons. The infestation rate was higher in Industrial area (97.50%) as compared to other regions. The highest prevalence of ectoparasites (100%) was recorded from Sargodha district. There was significant (P 0.05) variation among number of ectoparasites on wing, chest, tail and neck within age groups, seasons and ecological zones. The occurrence of parasites in relation to area, age, health status, sex and season were significant. The infestation rate of parasites in rock pigeon is high in different districts of Punjab. It is recommended that these wild birds infested with multiple species of ectoparasites could be the potential source of infestations in domesticated birds if they come in contact with them. The contact of domesticated birds should be prevented from wild birds to minimize the chance of cross species transmission of ectoparasites.


Resumo O presente estudo foi realizado para estimar a prevalência e diversidade de ectoparasitas em pombos-das-rochas em diferentes regiões de Punjab, Paquistão. Um total de 120 aves foram capturadas de março de 2017 a fevereiro de 2019. Os ectoparasitas foram coletados por procedimentos padrão e preservados em etanol 70% contendo uma gota de glicerina. Os dados relativos à idade, estado de saúde, sexo, tipo de área, local de amostragem e época do ano foram coletados em formulário padronizado. Os ectoparasitas foram identificados com base nas características morfológicas por meio de chaves de identificação. Noventa e seis (80%) aves estavam infestadas com ectoparasitas. Um total de sete famílias e treze espécies de diferentes ectoparasitas foram observados. Principalmente, sete espécies de piolhos, duas espécies de moscas, uma espécie de carrapato e três espécies de ácaros foram recuperadas de aves infestadas. Os pombos fêmeas foram infestados mais frequentemente (89,02%) do que os pombos machos (60,52%). A prevalência encontrada foi maior no verão (100%) em comparação com as outras estações. A taxa de infestação foi maior na área Industrial (97,50%) em relação às demais regiões. A maior prevalência de ectoparasitas (100%) foi registrada no distrito de Sargodha. Houve variação significativa (P 0,05) entre o número de ectoparasitas na asa, tórax, cauda e pescoço dentro das faixas etárias, estações do ano e zonas ecológicas. A ocorrência de parasitas em relação à área, idade, estado de saúde, sexo e estação do ano foi significativa. A taxa de infestação de parasitas em pombo-correio é alta em diferentes distritos de Punjab. Recomenda-se que essas aves selvagens infestadas com várias espécies de ectoparasitas possam ser a fonte potencial de infestações em aves domesticadas se entrarem em contato com elas. O contato de aves domesticadas deve ser evitado com aves selvagens para minimizar a chance de transmissão cruzada de ectoparasitas.

8.
Braz. j. biol ; 83: e246887, 2023. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1285630

RESUMEN

Abstract The current study was carried out to estimate the prevalence and diversity of ectoparasites in rock pigeons in different regions of Punjab, Pakistan. A total of 120 birds were captured from March 2017 to February 2019. The ectoparasites were collected by standard procedures and preserved in 70% ethanol containing one drop of glycerin. Data related to age, health status, sex, type of area, sampling location and season were collected using a standardized form. Ectoparasites were identified based on morphological characteristics by using identification keys. Ninety-six (80%) birds were infested with ectoparasites. A total of seven families and thirteen species of different ectoparasites were observed. Mainly, seven species of lice, two species of flies, one species of tick and three species of mites were recovered from infested birds. The female pigeons were more often infested (89.02%) than male pigeons (60.52%). The prevalence was found higher during summer (100%) as compared to other seasons. The infestation rate was higher in Industrial area (97.50%) as compared to other regions. The highest prevalence of ectoparasites (100%) was recorded from Sargodha district. There was significant (P < 0.05) variation among number of ectoparasites on wing, chest, tail and neck within age groups, seasons and ecological zones. The occurrence of parasites in relation to area, age, health status, sex and season were significant. The infestation rate of parasites in rock pigeon is high in different districts of Punjab. It is recommended that these wild birds infested with multiple species of ectoparasites could be the potential source of infestations in domesticated birds if they come in contact with them. The contact of domesticated birds should be prevented from wild birds to minimize the chance of cross species transmission of ectoparasites.


Resumo O presente estudo foi realizado para estimar a prevalência e diversidade de ectoparasitas em pombos-das-rochas em diferentes regiões de Punjab, Paquistão. Um total de 120 aves foram capturadas de março de 2017 a fevereiro de 2019. Os ectoparasitas foram coletados por procedimentos padrão e preservados em etanol 70% contendo uma gota de glicerina. Os dados relativos à idade, estado de saúde, sexo, tipo de área, local de amostragem e época do ano foram coletados em formulário padronizado. Os ectoparasitas foram identificados com base nas características morfológicas por meio de chaves de identificação. Noventa e seis (80%) aves estavam infestadas com ectoparasitas. Um total de sete famílias e treze espécies de diferentes ectoparasitas foram observados. Principalmente, sete espécies de piolhos, duas espécies de moscas, uma espécie de carrapato e três espécies de ácaros foram recuperadas de aves infestadas. Os pombos fêmeas foram infestados mais frequentemente (89,02%) do que os pombos machos (60,52%). A prevalência encontrada foi maior no verão (100%) em comparação com as outras estações. A taxa de infestação foi maior na área Industrial (97,50%) em relação às demais regiões. A maior prevalência de ectoparasitas (100%) foi registrada no distrito de Sargodha. Houve variação significativa (P <0,05) entre o número de ectoparasitas na asa, tórax, cauda e pescoço dentro das faixas etárias, estações do ano e zonas ecológicas. A ocorrência de parasitas em relação à área, idade, estado de saúde, sexo e estação do ano foi significativa. A taxa de infestação de parasitas em pombo-correio é alta em diferentes distritos de Punjab. Recomenda-se que essas aves selvagens infestadas com várias espécies de ectoparasitas possam ser a fonte potencial de infestações em aves domesticadas se entrarem em contato com elas. O contato de aves domesticadas deve ser evitado com aves selvagens para minimizar a chance de transmissão cruzada de ectoparasitas.


Asunto(s)
Humanos , Animales , Masculino , Femenino , Parásitos , Columbidae , Pakistán/epidemiología , Prevalencia , Animales Salvajes
9.
Journal of Prevention and Treatment for Stomatological Diseases ; (12): 400-407, 2023.
Artículo en Chino | WPRIM | ID: wpr-964440

RESUMEN

Objective @#To investigate the effect of isoprene cysteine carboxymethyltransferase (ICMT) gene on the migration and invasion of salivary adenoid cystic cancer cells (SACC) and the related mechanism, to provide experimental evidence for molecular targeted therapy of SACC.@*Methods@# Adenoid cystic cancer cells SACC-LM and SACC-83 were cultured in vitro, and siRNA was transfected into human SACC-LM and SACC-83 cells (experimental group) by transient transfection of a liposome vector. A blank control group and negative control group were set up respectively (transfected NC-siRNA). qRT-PCR was peformed to measure the mRNA expression of ICMT and RhoA in each group after transfection and to determine the silencing efficiency. The expression of ICMT, membrane RhoA, total RhoA, matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and Rho associated with coiled helical binding protein kinase 1 (ROCK1) in each group was detected by Western blot. The proliferation abilityies of SACC cells was detected by CCK-8 assay. The migration and invasion ability of SACC cells were detected by comparing the relative healing area of cell scratch assay and the number of Transwell assay cells. @*Results@#After transfection of ICMT-siRNA into SACC-LM and SACC-83 cells, the expression of ICMT gene and protein in the experimental group was significantly decreased compared with the negative control group and blank control group (P<0.05), but there were no significant differences in the expression of RhoA gene and total protein among all groups (P>0.05). The expression of RhoA membrane proteins, ROCK1, MMP-2, MMP-9 in the experimental group was significantly decreased compared with that in the negative control group and blank control group (P<0.05). Cell proliferation ability was significantly decreased (P<0.05). The migration and invasion abilities were significantly decreased (P<0.05). @*Conclusion @#In vitro silencing of ICMT gene can effectively inhibit the migration and invasion of human SACC-LM and SACC-83 cells, and the mechanism may be related to RhoA-ROCK signaling pathway.

10.
Journal of Pharmaceutical Practice ; (6): 478-484, 2023.
Artículo en Chino | WPRIM | ID: wpr-984556

RESUMEN

Objective To investigate the effect and mechanism of astragaloside Ⅳ(AS-Ⅳ) activating ROCK/JNK to regulate autophagy in improving isoproterenol (ISO) induced myocardial fibrosis (MF) in mice. Methods The mice were randomly divided into control operation group (Control group), ISO induced myocardial fibrosis group (MF group), AS-Ⅳ treatment group (AS-Ⅳ group) and combination group of astragaloside IV and Y-33075 (ROCK inhibitor) (astragaloside IV+Y-33075 group). After repeated administration for 30 days. The serum levels of LDH, BNP, CTGF in each group were detected. The cardiac function was detected by ultrasound. Myocardial structure and tissue fibrosis degree in each group were detected by Sirius Red and Masson staining. Oxidative stress (ROS) levels in myocardial tissue of each group were detected by DHE staining and the expression of ROCK, JNK, Atg5, Beclin 1, and LC3 Ⅰ/Ⅱ in myocardial tissue were detected by Western blotting. Results Compared with AS-Ⅳ group, the EF value of AS-Ⅳ+Y-33075 group decreased and the degree of myocardial fibrosis increased (P<0.05). The serum level of LDH, BNP, CTGF increased and the level of ROS in myocardial tissue increased while the expression of ROCK, JNK, Atg5, Beclin 1, LC3 Ⅰ/Ⅱ decreased (P<0.05). Y-33075 could block the protective effect of AS-Ⅳ on myocardial injury induced by MF and inhibit the regulation of AS-Ⅳ on ROCK and JNK. Conclusion AS-Ⅳ could attenuate myocardial fibrosis in mice by activating ROCK/JNK signal and promoting autophagy.

11.
China Journal of Chinese Materia Medica ; (24): 6434-6441, 2023.
Artículo en Chino | WPRIM | ID: wpr-1008843

RESUMEN

This study aimed to investigate the effect and underlying mechanism of Poria cocos polysaccharides(PCP) on myocardial cell apoptosis in the rat model of myocardial ischemia-reperfusion injury(MI/RI). Male SPF-grade SD rats were randomly divided into a sham group(saline), a model group(saline), low-and high-dose PCP groups(100 and 200 mg·kg~(-1)), and a fasudil group(10 mg·kg~(-1)), with 16 rats in each group. Except for the sham group, the other four groups underwent left anterior descending coronary artery ligation for 30 min followed by reperfusion for 2 h to establish the MI/RI model. The myocardial infarct area was assessed by TTC staining. Histological changes were observed through HE staining. Myocardial cell apoptosis was evaluated using TUNEL staining. Serum lactate dehydrogenase(LDH), creatine kinase MB(CK-MB), interleukin-1β(IL-1β) and IL-18 levels, myocardial superoxide dismutase(SOD) activity and malondialdehyde(MDA) levels were detected by ELISA. Protein expression of B-cell lymphoma 2(Bcl-2), Bcl-2 associated X protein(Bax), cleaved caspase-3, Ras homolog gene A(RhoA), myosin phosphatase target subunit 1(MYPT-1), phosphorylated MYPT-1(p-MYPT-1), and Rho-associated coiled-coil forming kinase 1(ROCK 1) were measured by Western blot. Pathological staining of myocardial tissue revealed that in the model group, there was focal necrosis of myocardial tissue, myocardial cell swelling, unclear boundaries, and neutrophil infiltration. These pathological changes were alleviated in the low-and high-dose PCP groups and the fasudil group. Compared with the model group, the low-and high-dose PCP groups and the fasudil group showed significantly reduced myocardial infarct area and myocardial cell apoptosis rate. Compared with the sham group, the model group exhibited elevated serum LDH, CK-MB, IL-1β and IL-18 levels, increased MDA levels, relative protein expression of Bax, cleaved caspase-3, RhoA, ROCK1 and p-MYPT-1, and decreased myocardial SOD levels and Bcl-2 protein expression. Compared with the model group, the PCP groups and the fasudil group showed lowered serum LDH, CK-MB, IL-1β and IL-18 levels, decreased MDA levels, relative protein expression of Bax, cleaved caspase-3, RhoA, ROCK1 and p-MYPT-1, and increased myocardial SOD levels and Bcl-2 protein expression. PCP exhibited a certain preventive effect on myocardial tissue pathological damage and myocardial cell apoptosis in MI/RI rats, possibly related to the inhibition of the Rho-ROCK signaling pathway activation, thereby reducing oxidative stress and inflammatory responses.


Asunto(s)
Ratas , Masculino , Animales , Daño por Reperfusión Miocárdica/tratamiento farmacológico , Proteína X Asociada a bcl-2/metabolismo , Ratas Sprague-Dawley , Caspasa 3/metabolismo , Interleucina-18 , Wolfiporia , Transducción de Señal , Infarto del Miocardio/tratamiento farmacológico , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Forma MB de la Creatina-Quinasa , Apoptosis , Polisacáridos/farmacología , Superóxido Dismutasa/metabolismo , 1-(5-Isoquinolinesulfonil)-2-Metilpiperazina/análogos & derivados
12.
China Journal of Chinese Materia Medica ; (24): 5041-5048, 2023.
Artículo en Chino | WPRIM | ID: wpr-1008674

RESUMEN

To investigate the intervention effect and mechanism of Zhenwu Decoction on diabetic nephropathy(DN) mice of spleen-kidney Yang deficiency syndrome based on the Rho-associated coiled-coil kinase(ROCK)/IκB kinase(IKK)/nuclear factor-κB(NF-κB) pathway. Ninety-five 7-week-old db/db male mice and 25 7-week-old db/m male mice were fed adaptively for one week. The DN model of spleen-kidney Yang deficiency syndrome was induced by Dahuang Decoction combined with hydrocortisone by gavage, and then the model was evaluated. After modeling, they were randomly divided into a model group, high-dose, medium-dose, and low-dose Zhenwu Decoction groups(33.8, 16.9, and 8.45 g·kg~(-1)·d~(-1)), and an irbesartan group(25 mg·kg~(-1)·d~(-1)), with at least 15 animals in each group. The intervention lasted for eight weeks. After the intervention, body weight and food intake were measured. Serum crea-tinine(Scr), blood urea nitrogen(BUN), fasting blood glucose(FBG), urinary albumin(uALb), and urine creatinine(Ucr) were determined. The uALb/Ucr ratio(ACR) and 24 h urinary protein(UTP) were calculated. Renal pathological morphology was evaluated by HE staining and Masson staining. The levels of key molecular proteins in the ROCK/IKK/NF-κB pathway were detected by Western blot. Enzyme-linked immunosorbent assay(ELISA) was used to detect interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-8(IL-8), interleukin-10(IL-10), and tumor necrosis factor-α(TNF-α). Compared with the blank group, the model group showed increased content of BUN, uALb, and SCr, increased values of 24 h UTP and ACR, decreased content of Ucr(P<0.05), enlarged glomeruli, thickened basement membrane, mesangial matrix proliferation, inflammatory cell infiltration, and collagen fiber deposition. The protein expression of ROCK1, ROCK2, IKK, NF-κB, phosphorylated IKK(p-IKK), phosphorylated NF-κB(p-NF-κB), and phosphorylated inhibitor of NF-κB(p-IκB) increased(P<0.05), while the protein expression of inhibitor of NF-κB(IκB) decreased(P<0.05). The levels of inflammatory factors IL-1β, IL-6, IL-8, and TNF-α increased(P<0.05), while the level of IL-10 decreased(P<0.05). Compared with the model group, the groups with drug treatment showed decreased levels of BUN, uALb, SCr, 24 h UTP, and ACR, increased level of Ucr(P<0.05), and improved renal pathological status to varying degrees. The high-and medium-dose Zhenwu Decoction groups and the irbesartan group showed reduced protein expression of ROCK1, ROCK2, IKK, NF-κB, p-IKK, p-NF-κB, and p-IκB in the kidneys(P<0.05), increased protein expression of IκB(P<0.05), decreased levels of serum inflammatory factors IL-1β, IL-6, IL-8, and TNF-α(P<0.05), and increased level of IL-10(P<0.05). Zhenwu Decoction can significantly improve renal function and renal pathological damage in DN mice of spleen-kidney Yang deficiency syndrome, and its specific mechanism may be related to the inhibition of inflammatory response by down-regulating the expression of key molecules in the ROCK/IKK/NF-κB pathway in the kidney.


Asunto(s)
Ratones , Masculino , Animales , FN-kappa B/metabolismo , Interleucina-8 , Interleucina-10 , Factor de Necrosis Tumoral alfa/metabolismo , Interleucina-6 , Quinasa I-kappa B , Bazo , Irbesartán , Uridina Trifosfato , Deficiencia Yang/tratamiento farmacológico , Riñón/patología
13.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 110-116, 2023.
Artículo en Chino | WPRIM | ID: wpr-969605

RESUMEN

ObjectiveTo investigate the effects of Yishen Daluo prescription (YSDL) on Ras homolog(Rho)/Rho-associated coiled-coil containing protein kinase(ROCK)signaling pathway in mice with experimental autoimmune encephalomyelitis (EAE) based on the silencing of β-arrestin1 gene. MethodSixty C57BL/6 female mice were randomly divided into a blank group, a model group, a virus group, a YSDL group, a virus + YSDL group, and a prednisone acetate group (hormone group). The EAE model was induced in mice except for those in the normal group. Adeno-associated virus(AAV)solution (150 μL, 1×1011 vg·mL-1) was injected into the tail vein of each mouse in the virus group and the virus + YSDL group on the 4th day of immunization. Drugs were administered on the 8th day of modeling. Specifically, normal saline was given to the mice in the normal group,the model group,and the virus group at 10 mL∙kg-1, prednisone acetate suspension to those in the hormone group at 3.9 g∙kg-1,and YSDL to those in other groups at 20 g∙kg-1 for 14 consecutive days. The mice were weighed and scored every day. The neurological function scores of mice in each group were recorded every day after immunization. Hematoxylin-eosin (HE) staining was used to determine the inflammatory response and lesion location in the brain tissues and spinal cord tissues of mice. The protein expression of β-arrestin1,Ras homolog gene family member A(RhoA), and Rho-associated coiled-coil forming protein kinase Ⅰ(ROCK Ⅰ) in spinal cord and brain tissues of EAE mice was determined by Western blot. ResultCompared with the model group, the virus group and the virus + YSDL group showed decreased neurological function scores (P<0.01),and the YSDL group also showed decreased neurological function scores(P<0.05). HE results showed that there was obvious inflammatory reaction in the central nervous system (CNS) of the model group, which was alleviated to varying degrees in other groups compared with the model group. Western blot results showed that compared with the blank group, the model group showed increased protein expression levels of β-arrestin1, RhoA, and ROCK Ⅰ in the spinal cord tissues (P<0.01). Compared with the model group, the virus group, the YSDL group, the virus + YSDL group, and the hormone group showed decreased protein expression levels of β-arrestin1, RhoA, and ROCKⅠ in the spinal cord tissues (P<0.01). Compared with the blank group, the model group showed increased protein expression levels of β-arrestin1, RhoA, and ROCK Ⅰ in the brain tissues (P<0.01). Compared with the model group, the virus group, the YSDL group, the virus + YSDL group, and the hormone group showed decreased protein expression level of β-arrestin1 in the brain tissues (P<0.01), and the virus group and the YSDL group showed decreased protein expression levels of RhoA, and ROCKⅠ in the brain tissues (P<0.05). Additionally, the virus + YSDL group and the hormone group showed decreased protein expression levels of RhoA and ROCKⅠ in the brain tissues (P<0.01). ConclusionYSDL can improve the clinical symptoms of EAE mice and improve the inflammatory response of CNS. The mechanism is presumably attributed to the fact that YSDL inhibits the expression of β-arrestin1 in CNS,thereby reducing the expression of Rho/ROCK signaling pathway. Furthermore, YSDL may have a synergistic effect with the inhibition of β-arrestin1 gene expression.

14.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 1-8, 2023.
Artículo en Chino | WPRIM | ID: wpr-953917

RESUMEN

ObjectiveTo explore the intervention mechanism of Xiangsha Liujunzi Tang in rats with functional dyspepsia (FD) based on the Ras homolog gene family member A (RhoA)/Rho-associated coiled-coil containing protein kinase 2 (ROCK2)/Myosin phosphatase target Subunit 1 (MYPT1) pathway. MethodSixty male SD suckling rats in SPF grades were randomly divided into blank group (n=10) and model group (n=50). The comprehensive modeling method (gavage administration of iodoacetamide+exhaustion of swimming+disturbance of hunger and satiety) was used to replicate the rat model of FD. After successful replication of the model, the rats in the model group were randomly divided into model group, mosapride group, and high, middle, and low-dose Xiangsha Liujunzi Tang groups, with 10 rats in each group. Rats in the blank group and model group were given 10 mL kg-1·d-1 normal saline, those in the mosapride group were given 1.35 mg·kg-1·d-1 mosapride, and those in the high, middle, and low-dose Xiangsha Liujunzi Tang groups were given 12, 6, and 3 g·kg-1·d-1 Xiangsha Liujunzi Tang, respectively. The intervention lasted 14 days. The general living conditions of rats were observed before and after modeling and administration, and the 3-hour food intake and body mass of rats were measured. After intervention, the intestinal propulsion rate of rats was measured, and the pathological changes in the gastric tissue were observed by hematoxylin-eosin (HE) staining. The content of choline acetyl transferase (ChAT) and vasoactive intestinal peptide (VIP) in the medulla oblongata and gastric tissue homogenate was determined by enzyme-linked immunosorbent assay (ELISA), the distribution of adenosine triphosphate (ATP) enzyme in gastric antrum smooth muscle was observed by frozen section staining, and the protein expression levels of RhoA, ROCK2, and phosphorylated-myosin phosphatase target subunit 1 (p-MYPT1) in the gastric tissue were detected by Western blot. ResultCompared with the blank group, the model group had withered hair, lazy movement, slow action, poor general living condition, lower 3-hour food intake, body mass, and lower intestinal propulsion rate (P<0.05), whereas no obvious abnormality in gastric histopathology. In the model group, the content of ChAT in the medulla oblongata and gastric tissue decreased, the content of VIP in gastric tissue increased, the distribution of ATP enzyme in gastric antrum smooth muscle decreased significantly, and the protein expression levels of RhoA, ROCK2, and p-MYPT1 in the gastric tissue decreased significantly (P<0.05). As compared with the model group, the general living condition of rats in each intervention group was significantly improved, and the 3-hour food intake, body mass, and intestinal propulsion rate were significantly increased (P<0.05). There was no significant difference in gastric pathology in the intervention groups. The content of ChAT in the medulla oblongata and gastric tissue increased significantly, the content of VIP in the gastric tissue decreased, the distribution of ATP enzyme in gastric antrum smooth muscle increased significantly, and the protein expression levels of RhoA, ROCK2, and p-MYPT1 in the gastric tissue increased significantly (P<0.05). The intervention effect of Xiangsha Liujunzi Tang group on the above indexes was dose-dependent. ConclusionXiangsha Liujunzi Tang can effectively improve the general living condition and gastric motility of rats with FD, and its specific mechanism may be related to the activation of the RhoA/ROCK2/MYPT1 pathway in the gastric tissue to regulate smooth muscle relaxation and contraction and promote gastric motility.

15.
Artículo | IMSEAR | ID: sea-221245

RESUMEN

Illegal mining activities have been rampant since decades. These activities have literally changed the face of the landscape. The mountains have disappeared, surface have been left dotted with deep mining pits, villagers have to live a substandard life due to the negative effects of mining in the form of decreased water quality, poor air quality, mining related accidents, damaged houses because of stone blasting and much more. The authorities have turned a blind eye towards the common man's problem and thus the residents of nearby villages are left with no choice but to live under silence. The unscientific and illegal mining practices in the south- west region of Haryana in the districts of Mehandargarh, Dadri and Bhiwani has altered the physical landscape and affected the socio cultural life of nearby villages to a great extent. The study is a step in the direction of describing how an anthropogenic activity if done without any rules and order and in unscientific manner can affect the environment to unimaginable

16.
J Vector Borne Dis ; 2022 Jul; 59(3): 265-274
Artículo | IMSEAR | ID: sea-216882

RESUMEN

Background & objectives: This study focuses on modulating dexterity of some ecological variables of Aedes vittatus classically breeding in rocky habitats. The study provides a useful insight into ecological variables that underpin or hinder profuse breeding of Ae. vittatus in rock pools and its probable role in disease transmission. Methods: HANNA HI98129 pH/EC/TDS/TEMP meter was used in situ while standard protocols were used to determine other hydro-chemical variables. Aedes vittatus larvae were obtained with soup ladle and modified ladle dippers. D-frame net was used to capture macroinvertebrates while plankton net was used to obtain samples of microalgae. Tadpoles and water turtles were collected with fine mesh invertebrate net. Macrophytes were uprooted and identified at the Herbarium Unit, Department of Botany, Ahmadu Bello University, Zaria. The influence of physicochemical variables was correlated with distribution of Ae. vittatus using Principal Component Analysis. Regression and ANOVA were used to test for association between predictor variables and mosquito abundance and for the difference amongst inselbergs. Results: Linear larval density of Ae. vittatus in rock pools which tapered across Guinea savanna were obtained from twenty-one sites with average density of 139.6 in Sudan savanna. Guinea savanna had an average larval density of 75.5 with lower subsets of moving average densities compared to Sudan savanna. One hundred and sixty-one aquatic insects belonging to four insect orders cohabited rock pools with Ae. vittatus. Toads and frogs’ tadpoles were of Bufonidae and Pyxicephalidae families while water turtles belong to Emydidae. pH, TDS (ppm), EC (µs/cm) and alkalinity (mg/l) differed significantly (p<0.05) with the abundance of Ae. vittatus in rock pools. Temperature, depth, water hardness and total suspended solid had direct influence on the distribution of Ae. vittatus in rock pools across sites. Significant positive correlation exists between aquatic insects and abundance of Ae. vittatus. Hydroperiod length, concentration of nitrate and pH were determinants that leverage profuse breeding of Ae. vittatus and survival of rock pool biota. Interpretation & conclusion: Results revealed that the bearing influence of rock pool variables is inevitable for breeding of Ae. vittatus. A well defined measure of efficacy incorporating indigenous communities for sustained vector control on inselbergs will go a long way in decimating population of Ae. vittatus and limit the risk of spread of yellow fever hitherto areas not thriving.

17.
Indian J Ophthalmol ; 2022 Jun; 70(6): 2020-2023
Artículo | IMSEAR | ID: sea-224348

RESUMEN

Purpose: Ripasudil hydrochloride hydrate (0.4%) is the first Rho?associated protein kinase (ROCK) inhibitor eye drop that lowers intraocular pressure (IOP) by increasing conventional aqueous outflow through the trabecular meshwork and Schlemm’s canal. We aimed to evaluate the safety and efficacy of ripasudil in patients using the maximum topical anti?glaucoma medications and with uncontrolled IOP. Methods: In our prospective interventional study, we enrolled 27 eligible and consenting patients (46 eyes) who presented to us between January 2021 and June 2021. Ripasudil 0.4% was added as adjunctive therapy to the ongoing glaucoma treatment. On follow?up visits at 7 days, 15 days, 1 month, 2 months, and 3 months, the visual acuity, IOP with applanation tonometer, anterior segment, and fundus were evaluated. The IOP before and after the use of ripasudil eye drops was compared by paired t?test. Results: Among the 27 patients, 18 were males and 9 were females. A statistically significant reduction in IOP was noted at all time durations (P < 0.00001) with the maximum reduction at 3 months with all patients achieving their target IOP. No patient developed any side effects necessitating the omission of ripasudil. The most common adverse event noted was conjunctival hyperemia (22 patients), which was mild and transient. Conclusion: Ripasudil showed additional IOP?lowering effect with other antiglaucoma medications and exhibited no significant side effects.

18.
Rev. Assoc. Med. Bras. (1992) ; 68(5): 586-590, May 2022. tab
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1376178

RESUMEN

SUMMARY OBJECTIVE: Sepsis is a complex and serious medical condition resulting from the activation of an innate host response to infections. The etiology of sepsis is complex and can be influenced by genetic susceptibility. The purpose of the present study was to investigate a possible association of Rho-kinase 1 (ROCK1) gene polymorphism with sepsis in a Turkish population. METHODS: The study group consisted of 100 unrelated patients with sepsis and 100 healthy controls. Genomic DNA was isolated from peripheral leukocytes from EDTA-containing blood using the QIAamp DNA Blood Mini Kit. ROCK1 gene rs35996865 and rs112130712 (Lys1054Arg) polymorphisms were analyzed in genomic DNA using the LightCycler 480 II real-time polymerase chain reaction. RESULTS: There were no significant differences in allele and genotype frequencies for ROCK1 gene rs35996865 polymorphism between the patients with sepsis and control group (p>0.05). Additionally, no association was detected between the rs35996865 polymorphism and mortality in the patient group. No polymorphism was detected with ROCK1 gene rs112130712 (Lys1054Arg) in our study groups. CONCLUSIONS: Our data showed that there is no marked association between the rs35996865 polymorphism and sepsis. Therefore, these results suggest that ROCK1 gene rs35996865 polymorphism is not risk factor for the development of sepsis in the Turkish population.

19.
Int. j. morphol ; 40(1): 270-276, feb. 2022. tab
Artículo en Inglés | LILACS | ID: biblio-1385589

RESUMEN

SUMMARY: Dance is an attractive sport discipline in which participation is increasing every year, unfortunately, the prevalence of injuries is also increasing. Various dance disciplines and dance techniques require body control, often in extreme anatomical positions that place a heavy strain on the musculoskeletal system. The aim of this research was to analyze body asymmetries of four different dance disciplines (standard and Latin American dance - STLA, acrobatic rock and roll - RNR, breakdance - BD and hip hop - HH), by using anthropometric (InBody 720; Biospace Co., Ltd) and 3D body measurements (NX-16; TC2) to establish possible later discomforts and injuries. T-test was performed to find differences between left and right extremities in all four dance disciplines. Results of the study showed that asymmetries are present in all of the studied dance disciplines. When comparing dance disciplines, we found out that STLA dancers are the most prone to develop body asymmetries (six out of nine paired variables), mainly because of the closed position. The position itself is the asymmetry and in which dancers remain for a very long time while training the technique of each dance. As dance is known for its asymmetrical movement of the body and it is expected that some of the asymmetries will appear after a few years of training. For a better understanding of possible consequences of asymmetries in dancers' bodies, further and more detailed analysis within each dance discipline is required.


RESUMEN: El baile es una disciplina deportiva atractiva cuya participación aumenta cada año, sin embargo también aumenta la prevalencia de lesiones. Varias disciplinas de baile y las técnicas de baile requieren el control del cuerpo en posiciones anatómicas extremas las cuales ejercen una gran presión sobre el sistema musculoesquelético. El objetivo de esta investigación fue analizar las asimetrías corporales de cuatro disciplinas de baile diferentes (baile estándar y latinoamericano - STLA, rock and roll acrobático - RNR, breakdance - BD y hip hop - HH), utilizando técnicas antropométricas (InBody 720; Biospace Co., Ltd) y mediciones corporales en 3D (NX-16; TC2) para establecer posibles molestias y lesiones posteriores. Se realizó la prueba T para determinar diferencias entre los miembros izquierdo y derecho en las cuatro disciplinas de baile. Los resultados de este estudio mostraron que las asimetrías se encontraron en todas las disciplinas de baile. Al comparar las disciplinas de baile, descubrimos que los bailarines de STLA son los más propensos a desarrollar asimetrías corporales (seis de nueve variables pareadas), principalmente debido a la posición cerrada. La posición en sí es asimétrica y en la que los bailarines permanecen durante mucho tiempo mientras entrenan la técnica de cada baile. Como el baile es conocido por el movimiento asimétrico del cuerpo, es posible que algunas de las asimetrías aparezcan después de algunos años de entrenamiento. Se requiere un análisis más profundo y detallado dentro de cada disciplina de danza para una mejor comprensión de las posibles consecuencias de las asimetrías en los cuerpos de los bailarines.


Asunto(s)
Humanos , Masculino , Femenino , Adulto Joven , Composición Corporal , Antropometría , Baile , Peso Corporal
20.
Chinese Journal of Clinical Pharmacology and Therapeutics ; (12): 544-550, 2022.
Artículo en Chino | WPRIM | ID: wpr-1014846

RESUMEN

AIM: To investigate the effect of Rho kinase inhibitor Y-27632 on acute liver injury in sepsis. METHODS: Thirty-two healthy adult male SD rats were randomly divided into sham operation group (Sham group), sham operation+Y-27632 group (Sham+Y group), cecal ligation and perforation group (CLP group) and CLP+Y-27632 group (CLP+Y-27632 group), 8 animals in each group. The rat sepsis model was established by the CLP method, and the rat was euthanized 24 hours after the model was established, and the serum and liver tissue were collected. Hematoxylin-eosin (HE) staining was used to observe the pathological changes in the liver tissue of the rats in each group; Western Blot was used to detect the expressions of ROCK1 and downstream NF-κB proteins in the liver tissue of the rats in each group; immunohistochemical method The expression of ROCK1 protein in liver tissue of rats was detected; enzyme-linked immunosorbent assay (ELISA) method was used to detect the levels of serum liver function indexes ALT and AST, and the changes of IL-18, IL-10 and GSH contents in liver tissue homogenate. RESULTS: Compared with the Sham group, there was no significant change in the histopathology of the liver in the Sham+Y group. In the CLP group, the arrangement of hepatocytes was disordered, with a large number of inflammatory cells infiltrating. Compared with the Sham group, the expression of ROCK1 protein in the CLP and CLP+Y groups was increased (P0.05); compared with the CLP group, the content of IL-18 in the CLP+Y group was decreased (P<0.05), and the content of IL-10 and GSH was increased (P<0.05). CONCLUSION: Rho kinase inhibitor can alleviate acute liver injury in septic rats, which may be related to inhibiting the expression of ROCK1 and NF-κB proteins, reducing the inflammatory response of liver tissue, and reducing the level of liver oxidative stress.

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