RESUMEN
Objective To analyze the epidemiological characteristics of intestinal flora in children with rotavirus enteritis (RVE) in Qinghai, and to provide a basis for clinical treatment and prevention. Methods A total of 64 children with RVE were enrolled in the RVE group, and 62 healthy children who received physical examination in our hospital during the same period were enrolled in the healthy control group. Stool specimens were collected, and fluorescence quantitative PCR technology was used to detect 16S rRNA of feces specimens for intestinal floras including Bifidobacterium, Lactobacillus, Escherichia coli and Enterococcus. The contents of common intestinal flora in two groups were compared. The changes in the contents of four major intestinal bacteria in RVE children of different genders, ages, nationalities and onset seasons were analyzed. Results Compared with the healthy control group, fecal contents of Bifidobacterium and Lactobacillus in the RVE group were significantly reduced (P<0.05). There was a significant difference in the fecal contents of Bifidobacterium and Lactobacillus in RVE children with different ages (P<0.05). The fecal contents of Escherichia coli and Enterococcus in children with RVE from 0 to 1 years old were significantly lower than those in other age groups (P <0.05); (P<0.05). Fecal Bifidobacterium content in RVE children of Han nationality was significantly higher than that of Tibetan children, while Lactobacillus content was significantly lower than that of Tibetan children (P<0.05). The fecal contents of Bifidobacterium and Lactobacillus of RVE children in autumn and winter were significantly lower than those in other seasons (P<0.05). Conclusion In Qinghai, rotavirus enteritis has a great influence on the contents of Bifidobacterium and Lactobacillus in children's intestinal tract. The contents of main intestinal bacteria are different in children with rotavirus enteritis of different ages, nationalities and seasons.
RESUMEN
Rotavirus enteritis （RVE）， a common infectious disease of the digestive system， is commonly found in infants and young children. Its incidence is high， posing great threats to human health. At present， no specific drugs are available in western medicine and the symptomatic treatments like fluid infusion， anti-diarrhea， correction of electrolyte disorder， protection of gastrointestinal mucosa， and regulation of intestinal microecology have been employed. In addition to the poor therapeutic efficacy， they do not perform well in preventing the onset of RVE and shortening the course of disease. Traditional Chinese medicine （TCM） exerts the therapeutic effect against RVE via multiple targets， without inducing obvious adverse reactions and the cost is low. The specific mechanism of action has yet to be fully explained. Although there are some studies exploring the role of Chinese medicinal monomers in the pathogenesis of RVE， the types involved are still not enough. The effects of Chinese medicinal monomers on autophagy-related pathways fail to be uncovered， which is attributed to the lack of large-scale experimental data and clinical evidence. There are also many problems that cannot be ignored in the related research of Chinese medicinal compounds， taking the superficial exploration and limited scope for instance. Autophagy is a highly conserved biological phenomenon that involves a variety of signaling pathways. Its dysfunction is related to multiple pathological processes. Studies have shown that autophagy plays an important role in the pathogenesis of RVE， especially in the early stage of viral infection. Autophagy induces intestinal mucosal barrier damage， intestinal nerve dysfunction， and immune abnormality， resulting in the occurrence and development of RVE. In recent years， a large number of experimental studies have confirmed that TCM fights against circulatory， respiratory， digestive， and immune system diseases as well as tumors by intervening in autophagy. At the same time， a handful of studies have suggested that Chinese medicinal monomers and compounds regulate autophagy and interfere with viral replication by affecting related signaling pathways， thus playing a positive role in reversing the progression of RVE. However， at present， there are few studies on the regulation of autophagy by TCM in the treatment of RVE， and no systematic elaboration is available. This review aimed to summarize the role of autophagy in the pathogenesis of RVE and its intervention with TCM， in order to provide more theoretical and clinical evidence for the treatment of RVE with TCM.
RESUMEN
Objective To investigate the effect of montmorillonite powder combined with racecadotril granules on serum levels of interleukin-2 (IL-2),interleukin-6 (IL-6),interleukin-10 (IL-10) and high-sensitivity C-reactive protein (hs-CRP) in children with rotavirus enteritis.Methods From June 2014 to July 2016,76 children with rotavirus enteritis in the People's Hospital of Pujiang County were selected and randomly divided into control group and study group according to the digital table,with 38 cases in each group.The two groups were given basic treatment after admission,on the basis,the control group was treated with montmorillonite powder,and the study group was treated with montmorillonite powder combined with racecadotril granules.The two groups were treated for 3 days.The clinical efficacy,clinical symptoms (stool,stool frequency,dehydration,vomiting,fever) recovery time,serum IL-2,IL-6,IL-10 and hs-CRP levels at admission and after treatment,and the incidence of adverse reactions were statistically compared between the two groups.Results The clinical efficacy of the study group (mild constipation,rash,nausea) was better than the control group[19 cases(50.00％) vs.11 cases(28.95％),16 cases(42.11％)vs.15 cases(39.47％),3 cases(7.89％) vs.12 cases(31.58％)],the difference was statistically significant(Z =2.358,P ＜ 0.05).The recovery time of stool character[(45.58 ± 10.11) h],stool frequency [(44.96 ± 10.03) h],dehydration[(33.15 ± 10.27)h],vomit[(18.16 ±6.46)h],and fever[(25.51 ± 10.32)h]of the study group were less than those of the control group(t =9.618,8.344,8.557,3.921,9.487,all P ＜ 0.05).The serum levels of IL-2,IL-6,IL-10 and hs-CRP before treatment between the two groups had no statistically significant differences (all P ＞ 0.05).After treatment,the serum levels of IL-2 and IL-10 in the study group were higher than those in the control group,the serum levels of IL-6 and hs-CRP were lower than those in the control group,the differences were statistically significant(t =5.545,7.705,7.090,10.352,all P ＜ 0.05).The incidence rate of adverse reactions between the study group and the control group had no statistically significant difference(10.52％ vs.13.15％,x2 =0.000,P ＞ 0.05).Conclusion Montmorillonite powder combined with racecadotril granules in the treatment of rotavirus enteritis can effectively relieve the clinical symptoms,improve the serum inflammatory factors level,reduce the inflammatory reaction in vivo,improve the therapeutic effect and has high safety.
RESUMEN
Objective To investigate the clinical effect of Qingrelishi prescription in the treatment of infantile rotavirus enteritis (RE). Methods One hundred and twenty children with RE admitted to the Department of Intestinal Outpatient of Tianjin Children's Hospital from October to November 2018 were enrolled, and they were divided into a Qingrelishi prescription group and a western medicine treatment control group, 60 cases in each group. Qingrelishi prescription addition and/or subtraction was given to the Qingrelishi prescription group (composition of the prescription: pueraria lobata, radix pseudostellariae, poria cocos, fried atractylodes macrocephala, each 6 g, scutellaria baicalensis, coptis chinensis, amomum villosum, argastaches leaves, roasted licorice, each 3 g), 1 dose a day, orally taken 1/2 dose twice a day, in thermogravimetric patients, plaster and rhizoma phragmititis were added, in cases with dampness, rhizoma alismatis and rhizoma atractylodis were added, and in patients with vomiting, bamboo shavings and pinellia were added; the western medicine treatment control group was given Montmorillonite powder. After 7 days of treatment, the clinical symptoms, changes of laboratory indexes and clinical efficacy were observed in both groups. Results After treatment, the number of children with stool soft, cases with fecal times less than 3 times per day and cases with reduction of fecal times ≥ 50% in Qingrelishi prescription group were obviously more than those in the western medicine treatment control group (fecal property soft: 25 cases vs. 21 cases, fecal times less than 3 times per day: 28 cases vs. 22 cases, cases with reduction of fecal times ≥ 50%: 48 cases vs. 41 cases, all P < 0.05). The number of children with laboratory examination index of rotavirus antigen (RV-Ag) transformed into negative, the case number with pH value normal shown by blood gas analysis, cases with residual alkali (BE) normal and cases with urinary ketone body transformed into negative in the Qingrelishi prescription group were significantly higher than those in western medicine treatment control group (RV-Ag transformed into negative cases: 49 cases vs. 42 cases, pH value normal cases:48 cases vs. 42 cases, BE normal cases: 51 cases vs. 43 cases, urinary ketone body transformed into negative cases:52 cases vs. 44 cases, all P < 0.05). The total effective rate of Qingrelishi prescription group was significantly higher than that of the western medicine treatment control group [80.0% (48/60) vs. 68.33% (41/60)], the difference was statistically significantly (P < 0.05). Conclusion Qingrelishi prescription can significantly improve the clinical symptoms and laboratory examination indicators of children with RE, increase the total clinical efficacy, and its therapeutic effect is definite.
RESUMEN
Objective To observe the protective effect of Racecadotril combined with Succinate Injection on myocardial injury in children with rotavirus enteritis and explore its clinical significance.Methods Totally 116 cases of children with rotavirus enteritis from Huangshi Traditional Chinese Medicine ofEdong Healthcare Group were selected in this study.Patients were randomly divided into Racecadotril control group (n =58) and Racecadotril combined with Succinate Injection observation group (n =58).The levels of CK,CK-MB,LDH,IL-17,IL-6,and TNF-α were detected by ELISA analysis.The rates of clinic effects and adverse reaction were compared.Results There was no significant difference of CK,CK-MB,LDH,IL-17,IL-6,and TNF-α between two groups before treatment.After treatment,they were all decreased in two groups (P ＜ 0.05,0.01).However,they were all lower in observation group than those in control group after treatment (P＜0.05).The rate of clinic effects was 94.8％ in observation group,which was higher than 81.0％ in control group (P ＜ 0.05).The rate of adverse reaction was 8.6％ in observation group and 6.9％ in control group.There was no significant difference between two groups.Conclusion Racecadotril combined with Succinate Injection inhibits the inflammatory response to protect myocardial injury in RVE children,with significantly clinical efficacy and safety.
RESUMEN
Objective To investigate the therapeutic effect of probiotics and traditional Chinese medicine syndrome-differentiation treatment for children rotavirus enteritis. Methods One hundred and twenty cases of children with rotavirus enteritis were randomly divided into Chinese medicine group, probiotics group and control group,40 cases in each group. The 3 groups were given conventional treatment such as proper feeding, prevention of dehydration and correction of dehydration. Additionally, Chinese Medicine group was separately given modified Gegen Qinlian Decoction for patients with damp-heat syndrome, modified Huoxiang Zhengqi Decoction for patients with wind-cold syndrome, modified Baohe Pills for patients impaired by overeating, modified Shenling Baizhu Powder for patients with spleen deficiency syndrome;probiotics group received Probiotic Bifidobacterium Triple Viable Enteric Capsules;the control group was given oral use of Smecta. Daily defecation frequency and stool characteristics were recorded,and the clinical efficacy was analyzed on treatment day 3 and 5. Results(1)After treatment for 3 days,the total effective rate of the 3 groups arrived to 100%,the difference being insignificant between the 3 groups (P > 0.05);after treatment for 3 days, Chinese medicine group had higher markedly effective rate than probiotics group and control group,the difference being significant (P < 0.05). (2) After treatment for 3 and 5 days,the defecation frequency of the 3 groups was much reduced as compared with that before treatment (P < 0.05), and the improvement of defecation frequency in Chinese medicine group was superior to that in probiotic group and control group, the difference being significant (P <0.05). (3)The mean course of disease in Chinese medicine group was shorter than that in probiotic group and control group,the difference being significant (P < 0.05). Conclusion Traditional Chinese medicine syndrome-differentiation treatment exerts certain effect for the treatment of rotavirus enteritis, and can quickly reduce defection frequency and shorten the course of disease.
RESUMEN
The abuse of the antibiotics causes many negative effects of human ecological environment. Probiotics can be eaten as food additives and maintain the balance of theintestinal flora. Because of the various physiological functions,probiotics can be used as micro?ecological preparation to cooperating with prebiotics and synbiotics in keeping microecological balance and improving the health level of the host. Probiotics,which are composed of a sufficient amount of non?pathogenic bacteria,can maintain the micro?ecological balance by improving the microbial flora on the surface of the host mucosa. In view of the positive roles,probiotics,such as Bifidobacterium and Bacillus,are wildly used as adjuvant therapy preparation in clinical treatment .
RESUMEN
Objective:To evaluate the rationality of drug use in the children with rotavirus enteritis by the children drug utilization index ( cDUI) . Methods:A retrospective analysis was used to investigate the cDUI, DDDs and rationality of cimetidine, saccharomy-ces boulardiiand and ganciclovir used in 78 children with rotavirus enteritis. Results and Conclusion: The value order of DDDs was saccharomyces boulardii>cimetidine >ganciclovir. Moreover, the dosage of cimetidine and saccharomyces boulardii was relatively high, while that of ganciclovir was basically rational.
RESUMEN
Objective To investigate the changes and clinical significance of routine blood test in infants with rotavirus enteritis.Methods The white blood cell count (WBC),the platelet parameters (PLT),the platelet volume (MPV)and the platelet distribution width (PDW)were detected by automatic blood cell analyzer respectively in 314 infants with rotavirus enteritis before and after the therapy for 5 -7 days.The C -reactive protein (CRP)was also detected,then made comparison with the normal control group (40 cases).Results The WBC (10.77 ±4.20)× 109 /L,PLT(330.02 ±117.70)×109 /L,MPV(8.46 ±2.15)fL in the infants with rotavirus enteritis were higher than the WBC(7.69 ±2.06)×109 /L,PLT(298.45 ±57.19)×109 /L and MPV(7.52 ±0.85)fL in the control group before the therapy.The differences were statistically significant (t value =4.569,1.670,2.746,P =0.000, 0.000 and 0.047).The WBC(7.76 ±2.83)×109 /L and CRP(5.18 ±5.82)mg/L in the infants with rotavirus enteritis after the therapy were lower than the WBC(10.77 ±4.20)×109 /L and CRP(7.70 ±11.28)mg/L before the therapy(t value =9.794 and 2.810,P =0.000).Conclusion The WBC,PLT and CRP in infants with rotavirus enteritis were usually higher.But the raise of WBC and CRP could not be the only indication of using antibiotic in infants suffered from diarrhea.
RESUMEN
Objective:To investigate the clinical efficacy and safety of Reduning injections in the treatment of infantile rotavirus enteritis. Methods:Totally 102 cases of infantile rotavirus enteritis in our hospital from June 2012 to June 2013 were randomly divided into two groups, the control group had 50 patients, and the treatment group had 52 cases. Both groups were given conventional symp-tomatic treatment, the control group was given 10 mg·kg-1 ribavirin injections in 100ml glucose injections for intravenous infusion, qd, and the treatment group was given 0. 5 ml·kg-1 Reduning injections in 100ml glucose injections for intravenous infusion, qd. Ev-ery 3-day was a course of treatment. The clinical curative effect of the two groups was observed, and the clinical symptoms, hospitaliza-tion time and adverse reactions were also studied. Results:After the treatment, the total effective rate in the control group was 70. 0%, while that in the treatment group was 92. 3%, and there was significant difference between the two groups (P<0. 05). The clinical symptom disappearance time and hospitalization time in the treatment group were significantly lower than those in the control group with statistically significant difference (P<0. 05). During the treatment, the control group had 5 cases of leucopenia and 2 cases of skin rash, and the patients all recovered after withdrawal of the drug, while the treatment group showed no obvious adverse reaction. Con-clusion:The curative effect of Reduning injections in the treatment of infantile rotavirus enteritis is promising, which can effectively improve the clinical symptoms, shorten the course of disease and reduce adverse reactions with the value of clinical application.
RESUMEN
Objective To study the effect of retention enema with Huanghu Decotion on infantile rotavirus enteritis and summarize the nursing strategies.Method One hundred and sixty nine infants with infantile rotavirus enteritis were randomly divided into observation group(n=86)and control group(n=83).On the basis of conventional treatment,the observation group was treated with retention enema with Huanghu Decotion and the control group with Smecta 3 d for a course of treatment.The two groups were compared in terms of the total effective rate.Results There was significant difference in the total effectives rate between the two groups(P<0.05).The rate in the observation group was highter than that in the control group.Conclusions Retention enema with Huanghu Decotion is superior to that by Smecta in treating infantile rotavirus enteritis.The comprehensive nursing care is helpful for the improved curative compliance and therapeutic effect.
RESUMEN
Objective To observe the clinical effect of interferon and Jinshuangqi in the treatment of infantil rotavirus enteritis and its effect on immune function.Methods 70 infantil rotavirus enteritis children met inclusion criteria were randomly divided into observation group (n =35) and control group (n =35).Both groups were given conventional treatment,the control group was given Jinshuangqi,the observation group was given interferon on the basis of control group.The two groups had been treated for 7d.The symptoms were observed.Serum immunoglobulin and T lymphocyte before and after treatment were detected,and the clinical efficacy was evaluated.Results The total effective rate of the observation group and the control group was 91.43％,77.14％,the difference was statistically significant(x2 =8.468,P ＜0.05).Antidiarrheal time of observation group and control group was (35.3 ± 11.2) d,(48.6 ± 15.6) d respectively,the difference was statistically significant (t =6.952,P ＜ 0.01).After treatment,IgG,IgA,IgM,CD3,CD4,CD8,CD4/CD8 in the observation group were (5.55 ± 3.58) g/L,(0.46 ± 0.05) g/L,(0.93 ±0.36) g/L,(63.4 ± 7) ％,(44.8 ± 6.4) ％,(25.2 ± 3.5) ％,(1.68 ± 0.20),those in the control group were (4.53 ±3.02)g/L,(0.36 ±0.04) g/L,(0.76 ±0.31)g/L,(62.1 ±6.7)％,(39.7 ±6.1)％,(30.3 ±4.2)％,(1.22 ± 0.25) respectively.The differences of IgG,IgA,IgM,CD4,CD8,CD4/CD8 between two groups were statistically significant(t =3.856,3.117,3.069,3.135,3.111,3.646,all P＜0.05).Conclusion Combined treatment with interferon and Jinshuangqi have exact clinical efficacy,can synergistically improve the immune fimction,restore the cellular and humoral immune,adjust the balance of intestinal flora,build a non-immune gut defense barrier,which is worthy of clinical application in the treatment of infantil rotavirus enteritis.
RESUMEN
Objective To explore the influence of the clinical pathway to control on hospital stay and medical expenses of pediatric rotavirus enteritis.Methods 245 infants with IRE were randomly assigned into two groups,109 in research group,136 in control group.All were treated with conventional therapy,for research group with clinical pathway.136 patients with previous medical routine therapy patients in the control group were compared.Compared two sets of average day in hospital,cure rate and cost,expenses for medicine and checking expenses proportion.Results The results shwed that the observation group of children's average hospital stay was lower than the control group,the cure rate higher than that in the control group,each time of total cost,expenses for medicine,each day medicine reducing( P ＜ 0.05 ).Checking expenses proportion was increased ( P ＜ 0.05),as to therapeutic effect,there was significant difference between two groups( P ＜ 0.05 ).Both expenses of drug ratio and ratio of the average daily expenses of drug are reduced.Conclusion Clinical pathway is applied in pediatric rotavirus enteritis can improve recovery rate,reduce hospitalization time and lower hospitalization fees and expenses,charges,medicine and inspection charge proportion.And can improve health education effect and patient satisfaction.
RESUMEN
Objective To evaluate the clinical effect of treating intractable rotavirus enteritis with Wenpi Yiyang Decoction. Methods Sixty cases with intractable rotavirus enteritis were recruited in this study and divided into a treatment group and a control group randomly.Traditional Chinese drugs of warming spleen and invigorating yang were adopted in the treatment group(30 cases).Results The total effective rate was 93% in the treatment group and 49% in the control group.There was no significant statistical difference between two groups(P＜0.05).Conclusion There was good clinical effect of treating intractable rotavirus enteritis with Wenpi Yiyang Decoction.
RESUMEN
Objective To explore the change of serum zinc level of children with rotavirus(RV) enteritis and efficacy of zinc supplementation on children with rotaviral enteritis.Methods One thousand one hundred and eighty-three healthy children were enrolled in this study,who were divided into different groups,and the zinc levels of children in every different age group were detected.These levels were used as normal levels of children in Dongguan district.One hundred and forty-three diarrhea patients in this 2 years were enrolled.Confirmed dia-gnosis was depended on the RV test in patients′ stool.The children with diarrhea were randomly divided into zinc treatment group (n=72) and conventional therapy group (n=71).The serum zinc levels in RV enteritis was analyzed.The patients in conventional therapy group were received diarrhea conventional therapy inchuded smectite and fluid infusion and so on;in addition to diarrhea conventional therapy,zinc gluconate 20 mg/d (10 mg/d below 6 months of age) for successive 14 days were given the patientsin zinc treatment group.The different efficacy of the 2 groups and the changes of serum zinc in treatment group were analyzed.Results Compared with normal children,the ones suffering from RV enteritis had lower serum zinc level(P0.05).Conclusions The ones suffering from RV enteritis have lower serum zinc level.In addition to diarrhea conventional therapy,giving zinc treatment has better effect on enhancing curative effect of RV enteritis.On the other hand,the suggestion of zinc supplement made by WHO is also suitable for Chinese children.
RESUMEN
Objective To observe the effect of herbal decoction for invigorating the spleen to dissolve damp on infantile rotavirus enteritis (IRVE) and myocardial enzymes.Methods Eighty patients were randomized into a treated group and a control group.The former was treated with the herbal decoction which could invigorate the functions of spleen to dissolve damp,the latter treated with Smecta.The course of treatment for both groups was 3 days.The changes in the symptoms of diarrhea,vomiting,and fever were observed and the myocardial enzymes including aspartate aminotransferase (AST),lactic dehydrogenase (LDH),creatine phosphokinase (CK) and CK-MB were determined.Results The effect of the treated group in stopping diarrhea,relieving fever,and stopping vomiting was all superior to that of the contro1 group (P0.05).Conclusion The herbal decoction for invigorating the spleen to dissolve damp in the treatment of IRVE functions to shorten the course of disease,improve the symptoms,and reduce the damage of cardiac muscles.
RESUMEN
Objective To study the clinical features of children with rotavirus enteritis complicated with respiratory infection.Methods The clinical features of 32 children with rotavirus enteritis were evaluated retrospectively complicated with respiratory infection (respiratory infection group) and 37 children with rotavirus enteritis complicated without parenteral infection (control group).Results 1. The respiratory symptoms became alleviative as the alimentary symptoms changed for better. 2. Duration of diarrhea weve(7.06?1.50)d in respiratory group was significantly longer than that in control group (4.73?1.31)d (t=6.90 P