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Aim To investigate the sensitization effect of total flavonoids of litchi seed (TFLS) to paclitaxel (PTX) on prostate cancer paclitaxel resistance (PCa-Txr) cells and the synergistic inhibitory effect of TFLS combined with PTX. Methods CCK-8 method was carried out to detect the inhibitory effect of TFLS and PTX on PCa-Txr cells as well as corresponding parental cells. PCa-Txr cells were either pre-treated or simultaneously treated with low cytotoxic dose of TFLS to observe the sensitivity of PCa-Txr cells to PTX. The synergistic inhibitory effect of TFLS combined with PTX on the proliferation of PCa-Txr cells were observed according to the method recommended by Chou T C. Combination index (CI) was used to determine whether TFLS and PTX had synergistic inhibitory effect. Results TFLS significantly inhibited the proliferation of PCa-Txr cells and parental cells in a certain time- and dose-dependent manner. The low dose of TFLS pre-treated or treated with PTX simultaneously failed to increase the sensitivity of PTX on PCa-Txr cells. TFLS combined with PTX significantly inhibited the proliferation of PCa-Txr cells with CI value less than one. Conclusions TFLS inhibits the proliferation of PCa-Txr cells, while TFLS combined with PTX has synergistic inhibitory effect on PCa-Txr cells. However, TFLS fails to increase the sensitivity of PCa-Txr cells to PTX.
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Objective: To clarify chemical dynamic change of volatile oil of Root of Changium Smyrnioides Wolff during Processing, to analyse and study the change of constituent proportion to see if new constituents produce or original constituents disappear, to discuss the processing mechanism of root of Changium smyrnioides . Method: The constituents and contain of volatile oils of root of Changium smyrnioides before, during and after processing are comparatively analyzed by using techniques of combined capillary gas chromatography mass spectrometry computer. Result: 34 chemical compounds are identified, among them there were 29 compounds isolated from volatile oil of fresh root of Changium smyrnioides, with a proportion of 97.873% in total oil, its main constituent is CSY and its content reaches 70.946%; 34 compounds were isolated from volatile oil of fresh decorticated root of Changium smyrnioides, with a propoction of 93.452%, main constituent is CSY, its content reaches 75.909%; and 19 compounds from volatile oil of fresh root cortices of Changium smyrnioides, 96.878% , main constituent is CSY, content 70.977%; 11 compounds from volatile oil of processed root of Changium smyrnioides, 88.839%, main constituent is CSY, content 67.234%. Conclusion: Main reasons of non sensibilization of processed root of Changium smyrnioides are considered to be the content decrease and qualitative change of the volatile oils, and the content of snsibilization activity constituent——CSY decreased from 0.0497% to 0.0134%.