Content Ditermination of Sulfer Dioxide Residues, Extracts and Main Chemical Compositions in Pinelliae Rhizoma from Different Producting Areas / 中国中医药信息杂志

Objective To determine the content of sulfur dioxide residues, extracts and main components; To study the effects of sulfur fumigation on the characters, extracts and index components of Pinelliae Rhizoma. Methods The contents of succinic acid, adenosine and guanosine in 22 samples of Pinelliae Rhizoma from different producing areas were detected by RP-HPLC method. The extracts of Pinelliae Rhizoma were measured by cold-maceration. Sulfur dioxide residues in Pinelliae Rhizoma were examined by acid-base titration. Results Among the 22 samples of Pinelliae Rhizoma, the ratio of sulphur smoked products of Pinelliae Rhizoma was 81.8％. Three kinds of components of succinic acid, adenosine and guanosine were well separated, baseline separation was achieved, and the average recoveries of samples were 99.1％, 100.1％ and 99.3％, respectively. Conclusion The phenomenon of sulfur fumigation is very serious in feild processed products of Pinelliae Rhizoma. To some extent, sulfur fumigation has an influence on the characters, extracts and the content of succinic acid in Pinelliae Rhizoma. This study provides references for quality standard revision of Pinelliae Rhizoma in Chinese Pharmacopoeia 2020.

Synthesis and application of a fluorescent molecular probe for rapid detection of sulfur dioxide residues in traditional Chinese herbs / 中国药科大学学报

@#A fluorescence method based on a “turn-on” rhodamine derivative fluorescent probe was developed for the detection of hydrogen sulfite ions and sulfur dioxide residues in sulfur-fumigated herbs. This method was realized through a well-known aldehyde-bisulfite addition reaction accompanied by a ring opening of spirolactam of probe molecule in pH 4. 8 aqueous ethanol media which resulted in a significant fluorescence and color change. The fluorescence enhancement was linearly proportional to the concentrations of bisulfite ranging from 0. 005 to 20 μmol/L with a correlation coefficient of 0. 998 5. The detection limit was low as to 2. 0 nmol/L and there was no interference with other familiar co-existing anions, cations and reducing reagents indicating high sensitivity and selectivity of the proposed method. In addition, the novel probe was successfully applied for the identification of sulfur dioxide residues in herbs with a satisfactory recovery in five real samples.