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Aim To analyze the anti-A549 and HI299 lung ade-nocarcinoma activities via using examples of baicalin, astragalo-side, hesperidin and cisplatin based on real time cellular analysis (RTCA) technology, and to build a new strategy for EC50 e-valuation reflecting the time-dimensional characteristic. Methods Using RTCA Software Pro for data analysis and GraphPad Prism and Origin Pro plotting, the in vitro anti-A549 and H1299 lung adenocarcinoma activities of baicalin, astragaloside, hesperidin, and cisplatin were characterized using the endpoint method and time dimension, respectively. Results (X) There were significant differences in EC50 values of A549 and H1299 cells at 24 h and 48 h endpoint methods. (2) The correlation coefficient of the curve fitted with the four-parameter equation was > 0. 9, and the dynamic change of EC50 remained relatively stable (the linear fitting of EC50 at adjacent 4 points I slope 1
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The characteristics of the pathogenic summer-heat and the primary important element of the season time dimension present parallelism principle on the forming mechanisms of five supremacies principal syndromes of initial summer febrile disease and four clinical characteristics of pathogenic factor summer heat. To explore the characteristics and the five successive evolutive phases corresponding to five elements phase theory of season and seasonal periods is provided with decisive guiding effect of passing through overall situation in the course of treating heat diseases caused by exogenous pathogenic factor based on syndrome differentiation in terms of the syndrome recognizing,establishing therapy principle and selecting Chinese materia medica to from a formula.It also presents effective resources and thoughts used for reference on the "bird flu" of nowadays,influenza of the human being(epidemic) that is likely to break out and how to treat these super-variations of virus(pestilential qi) based on syndrome differentiation with traditional Chinese medicine.
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The pharmacodynamic methodology of Chinese materia medica (PMCMM) at present was discussed comprehensively in this article and the problems were found While the primary study on the PMCMM was carried out This article offers that the development of the science of Chinese traditional medicine is the premise and basis of the study of PMCMM, especially that the breakthrough of modern study on the theory of viscera state and channel theory and channel tropism theory leads dominantly the study of PMCMM; the four dimensional system theory has been investigated in PMCMM, on the two dimensional basis of the physical foundation of medicinal efficacy and the mechanism of medicinal efficacy, the time dimension and human spirit dimension are added