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Objective To investigate the effects of Pien Tze Huang on the biological behavior of tongue squamous carcinoma Tca8113 cells and its molecular mechanism.Methods Tca8113 cells were incubated with Pien Tze Huang at different doses.The proliferation of Tca8113 cells was examined by MTT assay.The distribution of the cell cycle was evaluated by PI staining.The expression of the p-STAT3 and Bcl-2 proteins was assessed by Western blotting.Results MTT assay showed that Pien Tze Huang inhibited the proliferation of Tca8113 cells in a dose-dependent manner.PI staining showed that Pien Tze Huang arrested cells in the G0/G1 phase.The results of Western blotting suggested that Pien Tze Huang inhibited STAT3 protein phosphorylation and Bcl-2 protein expression.Conclusion Pien Tze Huang inhibits the proliferation of tongue squamous carcinoma cells.
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<p><b>OBJECTIVE</b>This study aimed to construct a eukaryotic expression vector pEGFP-N1-EMP1 of epithelial mem-brane protein 1 (EMP1) and investigate its influence on migration and invasion of human oral tongue squamous carcinoma cells.</p><p><b>METHODS</b>The human EMP1 gene was amplified by reverse transcription polymerase chain reaction and then ligated into the pEGFP-N1 vector by double restriction endonuclease digestion to construct pEGFP-N1-EMP1 recombinant plasmid. After sequencing identification, pEGFP-N1-EMP1 recombinant plasmid and pEGFP-N1 plasmid were transfected into human oral tongue squamous carcinoma Tb3.1 cell line. The expression of green fluorescent protein in cells was observed after transfection using an inverted fluorescence microscope. The overexpression of EMP1 mRNA was identified at 24, 48, and 72 h after transfection by real-time fluorescence quantitative polymerase chain reaction. The effect of EMP1 overexpression on migration and invasion of Tb3.1 cells was detected by Transwell assay.</p><p><b>RESULTS</b>The full-length EMP1 gene sequence was successfully obtained. Sequence analysis showed that the EMP1 gene was inserted into the pEGFP-N1 vector correctly. Green fluorescence was observed in the transfected cells under fluorescence microscopy. The results of real-time fluorescence quantitative polymerase chain reaction indicated that the expression of EMP1 at 24 h after pEGFP-N1-EMP1 transfection was significantly higher than the other groups. Transwell assays indicated that overexpression of the EMP1 gene could significantly inhibit the migration and invasion ability of Tb3.1 cells.</p><p><b>CONCLUSIONS</b>The eukaryotic expression vector of EMP1 was successfully constructed, and EMP1 overexpression was confirmed to inhibit the migration and inva-sion of oral tongue squamous carcinoma cells in vitro. This study laid a foundation for further investigation on the influence of the EMP1 gene on the metastasis of oral tongue squamous carcinoma and its molecular mechanism. .</p>