1.
Chinese Journal of Biochemistry and Molecular Biology
;
(12): 58-64, 2022.
Artículo
en Chino
| WPRIM
| ID: wpr-1015738
RESUMEN
Tripartite motif-containing protein 15 (TRIM15) is a member of the TRIM family, which is a class of proteins with E3 ubiquitin ligase activity. The function of TRIM15 in tumors is rarely reported. This study is intended to explain the role of TRIM15 in hepatocellular carcinoma. Nuclear and cytoplasmic fractionation and immunofluorescence assays confirmed that TRIM15 was located in the nucleus and cytoplasm. We designed hairpin RNA (shRNA) to knockdown TRIM15 in hepatocarcinoma cell lines. After knocking down TRIM15, cell growth curve and clone formation assays showed that cell proliferation was significantly inhibited (P<0. 05). Cell cycle analysis by flow cytometry showed that knockdown of TRIM15 blocked cell cycle in the G