Human umbilical cord mesenchymal stem cells combined with immunotherapy for the treatment of type 1 diabetic mice / 中国组织工程研究

BACKGROUND: Type l diabetes mellitus is a T cell-mediated autoimmune disease resulting in pancreatic islet cell damage. In this study, immunotherapy was used to deal with type l diabetes mellitus and stem cell transplantation was used to repair damaged islet p cells, attempting to explore a new treatment for type l diabetes mellitus. OBJECTIVE: To observe the efficacy of human umbilical cord mesenchymal stem cells combined with immunotherapy for the treatment of type l diabetic mice. METHODS: Fifty BALB/c Foxp3-DTR-EGFP positive mice were selected, six of which were randomly selected as normal control group and the remaining of which were intraperitoneally injected with streptozotocin and diphtheria toxin to prepare an animal model of type l diabetes mellitus. After successful modeling, randomization was performed in model mice and there were four groups: model group (normal saline), immunotherapy group (subcutaneous injection of dexamethasone (10 μg) and insulin (10 μg) mixture), cell transplantation group (injection of human umbilical cord mesenchymal stem cells (1 X106 cells per mouse) through the tail vein, and combined treatment group (the combination of immunotherapy and cell transplantation as described above). At 4 weeks after treatment, changes in blood glucose, C-peptide, body mass, pancreatic histopathology and insulin-positive area were observed in each group. RESULTS AND CONCLUSION: (1) Compared with the normal control group, the blood glucose level of the model group increased (P < 0.01) the C peptide level and body mass decreased (P < 0.01), and the islet was severely atrophied, with decreased number of islet cells and reduced insulin-positive area. (2) Compared with the model group, the blood glucose level of the immunotherapy group decreased (P > 0.05), the C-peptide level and body mass did not change significantly (P > 0.05), the islet cells increased in number, and the insulin-positive area increased. (3) Compared with the model group, the blood glucose level of the cell transplantation group and the combined treatment group decreased (P > 0.05), the C peptide level and body mass increased (P < 0.05), the islet cells increased in number, and the insulin-positive area increased. These findings reveal that either human umbilical cord mesenchymal stem cells or immunotherapy can improve the islet function of type l diabetic mice, and the combination treatment has better outcomes.

Curcumin reduces the invasion and migration abilities of papillary thyroid carcinoma TPC1 cells / 医学研究生学报

Objective Comparatively few studies are reported on the invasion and migration of papillary thyroid carcinoma (PTC) TPC1 cells. This study was to investigate the effects of curcumin on the invasion and migration abilities of TPC1 cells and its possible action mechanisms.MethodsWe treated TPC1 single cell suspension with curcumin at the concentrations of 0 (DMSO solvent), 10, 20, 40, 60, 80 and 100 μmol/L. At 24 and 48 hours after exposure, we examined the inhibitory effect of curcumin on the cells by CCK8 assays, detected the migration and invasion abilities of the TPC1 cells by Transwell and wound healing assay, and determined the gene and protein expressions Glut1 and MT1MMP by RTPCR and Western blot, respectively.ResultsThere were statistically significant differences in the cell viability among different groups of the TPC1 cells (P<0.05) as well as in the cell migration ability at 24 hours between any two groups of the cells treated with curcumin at 0 μmol/L (\[0.842±0.096\] mm), 10 μmol/L (\[0.911±0.049\] mm), 20 μmol/L (\[0.926±0.107\] mm) and 40 μmol/L (\[1.076±0.093\] mm) (P<0.05) and at 48 hours (P<0.05). Statistically significant differences were also observed between any two of the 0, 10, 20 and 40 μmol/L groups in the number of migrated cells (196, 142, 57, and 17/100x visual field) (P<0.05) as well in the protein expression of Glut1 (0.786±0.112, 0.518±0.106, 0.359±0.121, and 0.266±0.087) (P<0.05) and the mRNA and protein expressions of MT1MMP (P<0.05).ConclusionCurcumin can inhibit the invasion and migration of thyroid papillary carcinoma TPC1 cells, which may be associated with the decreased expressions of Glut1 and MT1MMP.

Effect of Electroacupuncture with Low Frequency on Pain Sensitization of Rats Suffered from Spinal Nerve Ligation / 中国康复理论与实践

@#Objective To investigate the effect of electroacupuncture with low frequency on pain sensitization of neuropathic pain rats,and the possible mechanism of regulating peripheral pain sensitization. Methods Rat neuropathic pain model was established with the right L5 spinal nerve ligation. Zusanli (ST36) and Kunlun (BL60) were selected for electroacupuncture intervention. Mechanical allodynia, and the levels of stransient receptor potential vanilloid type 1 (TRPV1) and substance P (SP) in ipsilateral L5 and L4 dorsal root ganglion (DRG) were tested. A further validation experiment with TRPV1 agonist 6'-IRTX was carried out. Results Rats suffered from L5 spinal nerve ligation showed obvious pain sensitization. The levels of TRPV1 in ipsilateral L4 DRG and SP in ipsilateral L5 DRG were significantly elevated.Electroacupuncture with low frequency significantly relieved pain sensitization, and suppressed TRPV1 and SP increase. 6'-IRTX intraperitoneally administered significantly counteracted the anti-pain sensitization action of electroacupuncture with low frequency. Conclusion Electroacupuncture with low frequency is effective on neuropathic pain by intervening pain sensitization, which may be related to the regulation of DRG TRPV1 and SP.

Effect of pentoxifylline on Fas and FasL expressions in pancreas and spleen of NOD mice / 中华内分泌代谢杂志

NOD mice were treated with pentoxifylline (FTX) to investigate the incidence of cyclophosphamide-accelerated diabetes, the apoptosis and the insulin expression of β-cells and expressions of Fas or FasL mRNA in both pancreas and spleen. The results showed that incidence of diabetes in PTX group was significantly lower compared with control group (P<0.05). The apoptosis of β-cells was decreased in PTX group with higher insulin expression level in islet cells. The expression of FasL mRNA in pancreas of PTX group was lower than that of control group (P<0.05), and there was no significant difference in Fas mRNA expression between two groups. Both Fas and FasL mRNA levels in spleen of PTX group were much higher than those of control group (P<0.05 or P<0.01).

Regulation of Prolactin Gene Expression by Hypothalamic Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in the Female Rat Pituitary Gland / 대한내분비학회지

BACKGROUND: Pituitary adenylate cyclase-activating polypeptide (PACAP) plays the role of a hypophysiotropic factor, which regulates the synthesis and secretion of pituitary hormones through the hypothalamo-hypophysial portal system. No clear evidence has yet been reported regarding the regulation of prolactin (PRL) by PACAP. In the present study, we tested a hypothesis that PACAP regulates the synthetic machinery of PRL during the estrus cycle and pubertal process using intracerebroventricular (i.c.v.) injection of an antisense oligodeoxynucleotide (ODN) against type I PACAP receptor (PAC1). METHODS: An RNase protection assay (RPA) was used to determine the pattern of hypothalamic PACAP and PAC1 mRNA expressions during the estrus cycle. Antisense PAC1 ODN was administered via i.c.v. injection to the female rats in normal estrus cycle of pubertal process. Northern blot analysis was used to determine the mRNA ievel of PRL in the pituitary gland. RESULTS: 1) PACAP mRNA in the medial basal hypothalamus was significantly increased at the diestrus I, while PAC1 mRNA showed no significant change. 2) PRL mRNA level of pituitary was increased by an injection of antisense PAC1 ODN at the proestrus and estrus stages. 3) PRL mRNA level of pituitary was significantly decreased by antisense PAC1 ODN injection at stage of prepuberty and initiate puberty, while its level was increased at stage of puberty. CONCLUSION: These data suggest that PACAP suppresses PRL mRNA synthesis through the PAC1 signaling pathway in the certain estrus cycle environments. It may be also involved in the regulation of pituitary PRL gene expression during the pubertal process

Voice and Imaging Analysis After Thyroplasty Type l in the Treatment of Unilateral Vocal Paralysis / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES:There are several methods to improve voice quality in the patients with paralyzed vocal cord, such as thyroplasty type I, injection, arytenoid adduction. The goal of surgical medialization of the paralyzed vocal fold is to attain complete glottic closure. The purpose of this study is to evaluate the effectiveness of thyroplasty type I through qualifying the vocal function and glottal gap. MATERIALS & METHODS: We experienced 20 cases of unilateral vocal fold paralysis who underwent thyroplasty type I. We performed preoperative and postoperative videoimage analysis (normalized glottal gap area) and computer-assisted voice analysis in all patients. RESULTS: The glottal gap was significantly reduced after thyroplasty type I. Postoperative voice quality was characterized by an improved pitch and amplitude perturbation (jitter and shimmer), maximum phonation time and subglottic pressure . But noise to harmonic ratio was not improved. CONCLUSION: Thyroplasty type I is an effective method in the point of regaining glottal closure and vocal function.