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Aim To study on the protective effects on vein endothelial cell of scallop skite-glycosaminoglycan(SS-GAG)and the mechanism of anti-atherosclerosis action of SS-GAG.Methods The endothelial cell of human umbilical vein had been cultured in vitro, and we established an model of endothelial cell oxidative damage induced by oxidized low density lipoprotein (OX-LDL), MTT assay and chemical methods were used to test the influence of SS-GAG on proliferation activity of endothelial cell oxidative damage and analyze nitric oxide (NO) and endothelial nitric oxide synthase (eNOS).Results Oxidized lowdensity lipoprotein (OX-LDL) remarkably inhibited the ability of cell proliferation, decreased nitric oxide (NO) and endothelial nitric oxide synthase (eNOS) (P
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Objective To investigate the antithrombosis effects of Buyang Huanwu Decoction(BHD) and its active fractions on cultured vascular endothelial cells(VEC) following the stimulation of thrombin and their mechanism.Methods The human umbilicus vein endothelial cells(ECV 304) were cultured in media containing 10 U/mL thrombin and different dosese of drugs.The levels of tPA and PAI-1 were detected by ELISA,the expression of TF,TFPI,and TM mRNA was measured by RT-PCR,and the expression of PKC? was examined by immunohistochemical staining 24 h later.Results Compared to the control without any treatment,the release of tPA was increased evidently(P0.05).Compared to the data after the mere stimulus of thrombin,each dose of BHD increased the release of tPA(P0.05);And glycoside(1.25 mg/mL) increased the release of tPA(P
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Human vascular endothelial cells(VEC)were isolated from umbilicalyeins by trypsin digestion.Spleen cells from BALB/c mice having been im-munized with the isolated endothelial cells were fused with the mousemyeloma cell line SP2/0.After screening and subcloning,three differentmonoclonal antibodies(EC1,EC2,EC3)against human umbilical VEC wereobtained.These antibodies were proved to belong to immunoglobulin sub-classes(IgG_2,IgG_3,IgG_2).EC1 was shown to bind strongly to the vascularendothelial cell membrane,but not to react with peripheral lymphocytes andmonocytes in microcytotoxicity test.We also explored the possible applica-tion of the monoclonal antibodies in VEC typing.