Responses of tomato hybrid cultivars to soil application of humic acid under greenhouse conditions

Abstract Responses of three tomato (Lycopersicon esculentum Mill.) hybrids viz. 'Luanova', 'Savarona' and 'Tessera' to various doses (0, 0.5, 1.0, 1.5 and 2.0 g L-1) of soil application of humic acid (HA) were evaluated in terms of plant growth, productivity and quality of fruits, under greenhouse conditions, during 2011-12 and 2012-13. In addition to improved quality characteristics, higher values for yield and yield components were recorded for tomato fruits harvested from plants of 'Tessera' cultivar compared to those harvested from other hybrids tested, regardless of growing seasons. Plants from 'Luanova' cultivar were, however, earlier in fruiting with higher number of fruits per plant. Soil application of HA presented positive effects on yield and physicochemical quality of tomato fruits irrespective of the dose of HA and the cultivar investigated, in both seasons. Soil application of HA at higher dose 1.5 g L-1 exhibited better results in all the three tomato cultivars tested, in terms of their vegetative and reproductive growth, and significantly improved the physicochemical quality of their fruits. Conclusively, the tomato hybrid 'Tessera' was found suitable for cultivation under greenhouse conditions while soil application of aqueous solution of HA @ 1.5 g L-1 substantially resulted in higher production of quality tomato fruits.

Resumo Respostas de três híbridos de tomate (Lycopersicon esculentum Mill.) viz. 'Luanova', 'Savarona' e 'Tessera' para várias doses (0, 0,5, 1,0, 1,5 e 2,0 g L-1) de aplicação de ácido húmico (AH) no solo foram avaliadas em termos de crescimento da planta, produtividade e qualidade de frutas, em casa de vegetação, durante 2011-12 e 2012-13. Além das características de qualidade melhoradas, valores mais elevados para o rendimento e os componentes do rendimento foram registrados para frutos de tomate colhidos de plantas do cultivar 'Tessera' em comparação com aqueles colhidos de outros híbridos testados, independentemente das estações de cultivo. As plantas do cultivar 'Luanova', no entanto, frutificaram mais cedo com maior número de frutos por planta. A aplicação de AH no solo apresentou efeitos positivos sobre a produtividade e a qualidade físico-química dos frutos de tomate, independentemente da dose de AH e do cultivar investigado, nas duas safras. A aplicação de HA no solo na dose maior de 1,5 g L-1 apresentou melhores resultados nos três cultivares de tomate testados, quanto ao crescimento vegetativo e reprodutivo, e melhorou significativamente a qualidade físico-química dos frutos. Conclusivamente, o híbrido de tomate 'Tessera' foi considerado adequado para cultivo em casa de vegetação, enquanto a aplicação no solo de solução aquosa de HA @ 1,5 g L-1 resultou substancialmente em maior produção de frutos de tomate de qualidade.

Nutrient Management for Growth, Yield and Quality of Strawberry (Fragariax ananassa) in Vertical Hydroponics System

The present investigation was carried out to find out nutrient management for growth, yield attributes, yield and quality attributes of strawberry (Fragaria ananassa) under vertical hydroponics tower system in Prayagraj. The experiment was conducted in a Complete Randomized Design (CRD) with nine treatments and three replications during November-March (2022-23) at Experimental Farm of Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India. The data were recorded from four randomly selected plants from each replication of the treatments for 14 characters. The 9 treatments comprising of different doses in combination of macro nutrients N:P:K , micro nutrients Zn: B: Fe and organic fertilizer sea weed extract were given in three different stages of the crop i.e., seedling stage, vegetative growth stage and reproductive stage . Naphthalene Acetic Acid (NAA) as plant growth regulator (PGR) was applied in reproductive stage of the crop at the time of flowering for better fruit set. Aqueous solution of nutrients applied at seedling stage followed by vegetative growth stage and reproductive stage , treatment T7 comprising of N- 7.5, 8.5 and 9.5 g/10 litres of water at all three stages, respectively, P- 4.0, 5.0 and 6.0 g/10 litres of water at all three stages, respectively, K- 2.5, 3.5 and 4.5 g/10 litres of water, at all three stages, respectively, Zn- 0.25,0.35 and 0.45g/10 l of water at all three stages, respectively , B – 0.35, 0.45 and 0.55g/10 litres of water at all three stages, respectively, Fe- 0.65,0.75 and 0.85 mg/10 litres of water at all three stages, respectively and organic fertilizer (see weed extract) @ 8 and 10 ml/ 10 litres of water at seedling and vegetative stages and NAA @ 300 ppm was applied at reproductive stage for fruit settings was the best and recorded maximum plant height (18.42cm),plant spread area (18.58cm2), number of leaves per plant (8.21), root length (16.03cm), leaf area (47.75cm2), number of flowers per plant (14.47), number of fruits per plant (14), yield per treatment (1.451kg) with B: C ratio (1:2.72) and quality of the strawberry as TSS (9.330brix), titratable acidity (1.33%) and ascorbic acid (60.45 mg/100g) followed by T6 and T5.

Effect of Inorganic Fertilizer and Spacing on the Plant Growth of Dragon Fruit (Hylocereus costaricencis) under Tamil Nadu Agro-Climatic Conditions

The dragon fruit (Hylocereus costaricencis) is a new emerging fruit crop, there is very less information available on the spacing and fertilizer dose requirements of this crop so the present study was carried out to find the effect of fertilizer doses, spacings, and interaction effect of both on plant growth of dragon fruit (Hylocereus costaricencis) at the Orchard, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore. during the year 2022. The design of the experiment was a split plot with four main plots viz. M1 = 3.5 x 2.0 m2, M2 = 3.0 x 3.0 m2, M3 = 3.0 x 2.5 m2, M4 = 2.5 x 2.5 m2 and six sub plots viz. S1 = N540 P420 K360, S2 = N495 P385 K330, S3 = N450 P350 K300, S4 = N340 P260 K225, S5 = N225 P175 K150, S6 = N0 P0 K0 g per pillar was applied and replicate four times. A significant difference was observed in terms of vine length (m), cladode girth (cm), cladode length (cm), and cladode number when different doses of fertilizers were applied. The highest vine length (2.96), cladode girth (24.52), cladode length (67.21), and number of cladodes (67.48) were observed in S3 (N450 P350 K300). Similarly, the highest vine length (2.503) and cladode girth (19.78) were observed in M4 (2.5 x 2.5 m2). The highest cladode length (67.47) was observed in treatment combination M2S3. Hence, the application of fertilizer dose S3 (N450 P350 K300) and adoption of spacing M4 (2.5 x 2.5 m2) is good to enhance the vegetative growth characters of dragon fruit under Tamil Nadu conditions.

Efeito da Aloe arborescens Miller e da Aloe barbadensis Miller sobre o desenvolvimento vegetativo em Aspergillus nidulans / Effects of the Aloe arborescens Millerand Aloe barbadensis Milleron over vegetative growth in the Aspergillus nidulans

RESUMOA pesquisa de produtos naturais benéficos à saúde humana vem crescendo nos últimos 20 anos. Considerando que as plantas de Aloe são amplamente utilizadas pela população humana, em geral de maneira terapêutica, o objetivo deste estudo foi avaliar os efeitos de Aloearborescens Miller e Aloe barbadensis Miller, sobre o desenvolvimento vegetativo de linhagens normais e mutantes de Aspergillus nidulans. Conídios da linhagem biA1methG1, MSE e CLB3 de A. nidulans, foram inoculados em meio completo sem (Controle) e com extratos das duas espécies incubados por 2, 4, 6 e 8 horas a 37ºC, no escuro. Foi analisado em microscópio óptico, 200 conídios de cada tratamento. Para o desenvolvimento das colônias, as linhagens foram inoculadas no centro das placas juntamente com o meio de cultura sólido e sobre a membrana de diálise, visando a medição do diâmetro e do peso. A análise estatística foi baseada no teste de Tukey e todos os procedimentos experimentais foram conduzidos em triplicata. Todas as linhagens apresentaram interferências positivas quando expostas às plantas de Aloe, porém, de maneira variada. Ambas as espécies aceleraram a germinação em todas as linhagens testadas e atuaram na redução significativa de conídios mortos e/ou malformados. Em relação ao desenvolvimento vegetativo, todos os dados referentes ao peso úmido e diâmetro corrigido dos tratamentos demonstraram progressos, contudo, a razão diâmetro/peso apresentou somente na linhagem MSE, ação favorável dos tratamentos naturais. As informações deste estudo sugerem benefícios de A. arborescens e A. barbadensis, justificando a importância e continuidade da investigação, para melhor elucidar os mecanismos de ação dessas plantas.

ABSTRACTThe researches about natural products that arebeneficial to human health have been growing over the past 20 years. Since Aloe plants are broadly used by the general population, frequently due to therapeutic reasons, the objective of this study was to evaluate the effects of Aloe arborescens Millerand Aloe barbadensis Miller on the vegetative growth of normal and mutant strains of Aspergillus nidulans. The conidia of thebiA1methG1, MSE and CLB3 strains of A. nidulans were inoculated in complete environment without (control) and with extracts of two species of Aloeincubated for 2, 4, 6 and 8 hours at 37˚C. 200 conidia were analyzed by optical microscopy. For the development of the colonies, the strains were inoculated in the center of the plates together with the solid environment of the cultivation and over the dialysis membrane for measuring the diameter and weighing. The statistical analysis was based on the Tukey test and all experimental procedures were performed in triplicate. All strains showed positive interference when exposed to Aloe plants, however, through different manners. Both species have accelerated the germination in all tested strains and acted in the significant reduction of dead and / or malformed conidia. Regarding the vegetative growth, all data related to wet weight and corrected diameter of the treatments revealed progress, however, the ratio diameter/weightpresented improvement only in the MSE lineage, favorable action of natural treatments. The information from this study suggest that A. arborescens and A. barbadensis are beneficial, thus justifying the importance of research maintenance in order to better elucidate the action mechanisms of these plants.

Substratos e recipientes para a produção de porta-enxertos de citros irrigados por subcapilaridade / Substrates and containers for the citrus rootstocks' production irrigated by capillarity

O conhecimento do porta-enxerto, do substrato e do recipiente são fundamentais para a definição do período necessário para produzir mudas cítricas de qualidade. O objetivo deste trabalho foi avaliar a emergência de plântulas e o desenvolvimento vegetativo de seis porta-enxertos cítricos, cultivados em dois substratos e mantidos em diferentes recipientes, em sementeira, irrigados por subcapilaridade. Foram testados dois substratos comerciais e a semeadura foi realizada em tubetes com 50cm³, tubetes com 120cm³ e bandejas de isopor contendo 72 células com volume de 120cm³. Os seis porta-enxertos cítricos avaliados foram: Trifoliata [Poncirus trifoliata [L.] Raf.]; Flying Dragon [Poncirus trifoliata (L.) Raf. var. monstrosa]; limoeiro Volkameriano (Citrus volkameriana Pasq.), citrangeiro Fepagro C37 [P. trifoliata x Citrus sinensis [L.] Osbeck.], tangerineira Sunki (C. sunki hort. ex Tan.) e citrumeleiro Swingle (P. trifoliata x C. paradisi). Avaliou-se emergência, altura e diâmetro das plantas ao longo do cultivo em casa de vegetação. Em laboratório, avaliou-se massa fresca e seca da parte aérea e raiz, número de folhas, área foliar por folha e área foliar por planta. Nas avaliações de emergência de plântulas, a tangerineira Sunki, o limoeiro Volkameriano e o citrumeleiro Swingle apresentaram maiores taxas em relação ao Trifoliata, ao citrangeiro Fepagro C 37 e ao Flying Dragon. Ao empregar-se o sistema de irrigação por subcapilaridade, o substrato comercial 1 é recomendado para todos recipientes. O substrato comercial 2 somente deve ser empregado em bandejas alveoladas de 120 cm³.

In the production of citrus seedling, the knowledge of rootstock substrate and container is essential to define the entire substantial period to produce quality seedling. The objective of this research was to evaluate seedling emergence and vegetative development of six citrus rootstocks grown on two substrates and kept in different containers at the stage of sowing irrigated by capillarity. Two commercial substrates were tested and seeding was performed in three containers: stiff plastic tubes with 50cm³, stiff plastic tubes with 120cm³ and polystyrene trays containing 72 cells with a volume of 120cm³. The six rootstocks citrus evaluated were: Trifoliata [Poncirus trifoliata [L.] Raf.]; Flying Dragon [Poncirus trifoliata (L.) Raf. var. monstrosa]; Volkameriano lemon (Citrus volkameriana Pasq.), Fepagro C37 citrange [P. trifoliata x Citrus sinensis [L.] Osbeck.], Sunki tangerine (C. sunki hort. ex Tan.) and Swingle citrumelo (P. trifoliata x C. paradisi). The emergence, height and diameter of plants in the greenhouse were evaluated. Fresh weight, root and shoot dry weight, leaf number, leaf area per plant and leaf area per leaf were evaluated in the laboratory. In the evaluations of emergence of seedlings, the Sunki tangerine, Volkameriano lemon and Swingle citrumelo had higher rates compared to Trifoliata, Fepagro C37 citrange and Flying Dragon. When the system in a floating irrigation system, commercial substrate 1 is recommended for all containers. The commercial substrate 2 should be only used in alveolate trays of 120cm³.

Desempenho agronômico da cultura do nabo forrageiro influenciado pelos resíduos de plantas de girassol / Oil radish development agronomic affected by sunflower plants residues

O objetivo foi o de avaliar a emergência das plântulas, o crescimento das plantas, bem como a produtividade e a qualidade das sementes produzidas de nabo forrageiro (Raphanus sativus L.) influenciados pelos resíduos de plantas de girassol (Helianthus annus). Para isto, o delineamento experimental adotado foi o de blocos casualizados em parcelas subdivididas com quatro repetições. As parcelas foram representadas por área com e sem resíduos de plantas de girassol e, as subparcelas, por seis épocas de coleta (20, 40, 60, 80, 100 e 120 dias após a semeadura - DAS). Os resíduos de plantas de girassol não interferiram no estabelecimento das plântulas de nabo forrageiro, reduziram a produção de sementes por planta e favoreceram o vigor das sementes produzidas. Após 40 DAS, houve prejuízo ao desenvolvimento das plantas, à eficiência de translocação dos fotoassimilados e ao acúmulo de nutrientes de plantas de nabo forrageiro provenientes de área com resíduos de girassol.

The aim was to evaluate the seedling emergence, plant growth, seed yield and seed quality of oil radish (Raphanus sativus L.) influenced by the sunflower (Helianthus annus L.) plants residues. The experiment was randomized in split plot complete block with four replications. The plots were represented by the area with and without sunflower plants residues and the subplots, by the six sampling times (20, 40, 60, 80, 100 and 120 days after sowing - DAS). The sunflower plants residues did not affect seedling establishment in oilseed radish, reduced the seed yield per plant and favored the seed vigour. After 40 DAS, there was damage to the plant development, the efficiency of translocation of assimilates and nutrients accumulation in oil radish plants from area with sunflower residues.

Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes

The chemical changes in barley-straw (BS), wheat-straw (WS) and vineyard-pruning (VP) substrates were determined during colonization of Lentinula edodes mycelia (during primordium development) in solid state fermentation. Primordia appeared 39-50 days after inoculation. VP appeared to promote early sporophore initiation. The concentration of hemicellulose in BS and VP decreased gradually from 25.5 percent to 15.6 percent and from 15.8 percent to 12.3 percent, respectively. However in WS, hemicellulose decreased from 27.2 percent to 9.5 percent. Lignin broke down continuously in BS and WS, with 31.8 percent and 34.4 percent degradation, respectively; higher than that of cellulose. During the pinning stage, the C:N ratio decreased in VP and BS, but not in WS. On all substrates the phenols decreased notably throughout the first week of mycelial growth. The time elapsed (days) to pinning was positively correlated with cellulose content (r=0.89), total sugar (r=0.85) and inversely correlated to lignin (r=-1.00) and phenol content (r=-0.55).

Desenvolvimento vegetativo e acúmulo de massa seca com a adubação de porta-enxertos cítricos cultivados em tubetes / Vegetative growth and dry matter accumulation with fertiliz

Com o objetivo de avaliar o efeito de diferentes substratos e doses de solução nutritiva na formação dos porta-enxertos Poncirus trifoliata, citrangeiro 'Troyer', citrumeleiro 'Swingle' e tangerineira 'Sunki' em tubetes, realizou-se, sob condições de casa de vegetação, um experimento com delineamento experimental em blocos casualizados, em esquema de parcelas subdivididas com três repetições e cada subparcela constituída por 20 tubetes. Na parcela principal, foram testadas quatro doses de solução nutritiva (0; 0,25; 0,5 e 1,0g L-1 de adubo - relação N=1; P=0,14; K=0,34; Ca=1,41; e Mg=0,13, sendo a concentração de N na solução estoque de 7,2 por cento) aplicada via água de irrigação, em fertirrigação contínua, com sistema de irrigação por subirrigação. Nas subparcelas, foram testados quatro porta-enxertos cítricos e dois substratos comerciais. A solução nutritiva foi trocada a cada 15 dias. O máximo crescimento e acúmulo de massa seca foram, de um modo geral, alcançados na aplicação da dose máxima de solução nutritiva, com exceção da área foliar do 'Trifoliata' e massa seca da parte aérea da tangerineira'Sunki'. O substrato comercial 1 propiciou o maior crescimento e acúmulo de biomassa, independente dos porta-enxertos e das doses de fertilizante utilizados.

An experiment was carried out in the greenhouse aiming to evaluate the effect of different substrates and levels of nutrient solution in the formation of Poncirus trifoliata, 'Troyer' Citrange, 'Swingle' Citrumelo and tangerine 'Sunki' citric rootstock grown in containers. The experimental design was a randomized blocks in split plot scheme with three replications. Each sub-plot had twenty containers. The main plots received four levels of nutrient solution (0; 0.25; 0.5; 1.0g L-1 relationship N=1; P=0,14; K=0,34; Ca=1,41; Mg=0,13 and N concentration in the stock solution of 7.2 percent) applied by water irrigation in a continuous fertilization system in a floating irrigation system. The sub-plots tested four citric rootstocks and two commercial substrates. The nutritive solution was changed at 15-day intervals. The maximum growth and dry mass accumulation, in general, reached with the application of the maximum level of nutritive solution, except the foliar area of 'Trifoliata and dry mass of the aerial part of tangerine 'Sunki'. The commercial substrate 1 provided the greater growth and biomass accumulation, independently of stock for grafting and dosages of fertilizers used.

Compatibilidade de isolados de Beauveria bassiana (Ascomycetes: Clavicipitaceae) com carrapaticidas químicos utilizados no controle do carrapato dos bovinos / Compatibility of Beauveria bassiana (Ascomycetes: Clavicipitaceae) with chemicals acaricides used in the control of cattle tick

O presente trabalho teve como escopo avaliar a compatibilidade dos isolados IBCB66 e IBCB21 de Beauveria bassiana com os carrapaticidas a base de Flumetrina + Coumafós, Deltametrina, Diclorvós + Cipermetrina, Diclorvós + Clorpirifós, Cipermetrina High Cis, Diclorvós + Cipermetrina High Cis, Cipermetrina e Amitraz utilizados no controle do carrapato Rhipicephalus (Boophilus) microplus em nosso país. O efeito dos produtos comerciais sobre os isolados foi verificado por meio da observação do crescimento vegetativo, produção de conídios e viabilidade das estirpes do fungo B. bassiana. Com referência ao isolado IBCB66, os produtos Deltametrina, Cipermetrina High Cis e Amitraz foram classificados como compatíveis, não interferindo no desenvolvimento do entomopatógeno. O produto Cipermetrina foi classificado como tóxico e os produtos Flumetrina + Coumafós, Diclorvós + Cipermetrina, Diclorvós + Clorpirifós e Diclorvós + Cipermetrina High Cis, muito tóxicos. Em relação ao isolado IBCB21, os produtos Flumetrina + Coumafós, Diclorvós + Cipermetrina, Diclorvós + Clorpirifós, Cipermetrina High Cis, Diclorvós + Cipermetrina High Cis e Cipermetrina foram classificados como muito tóxicos e o produto Amitraz como tóxico. Entre os carrapaticidas avaliados, o produto Deltametrina foi o único que não produziu efeito tóxico sobre o entomopatógeno.

The purpose of the present study was to assess compatibility between IBCB66 and IBCB21 isolates of Beauveria bassiana and acaricides: Flumethrin + Coumaphos, Deltamethrin, Dichlorvos + Cypermethrin, Dichlorvos + Chlorpyrifos, Cypermethrin High Cis, Dichlorvos + Cypermethrin High Cis, Cypermethrin and Amitraz, utilized on the control of Rhipicephalus (Boophilus) microplus in our country. The effect of commercial products on the isolates was assayed according to observation of vegetative growth, conidia production, and viability of strains of B. bassiana fungus. With concerning about IBCB66 isolate, products Deltamethrin, Cypermethrin High Cis and Amitraz were compatible, not affecting the entomopathogen development. Product Cypermethrin was toxic, and products Flumethrin + Coumaphos, Dichlorvos + Cypermethrin, Dichlorvos+ Chlorpyrifos and Dichlorvos + Cypermethrin High Cis were very toxic. In regard to IBCB21 isolate, products Flumethrin + Coumaphos, Dichlorvos + Cypermethrin, Dichlorvos + Chlorpyrifos, Cypermethrin High Cis, Dichlorvos + Cypermethrin High Cis and Cypermethrin were very toxic and product Amitraz was toxic. From the acaricides evaluated, product Deltamethrin was the single agent that did not produce toxic effect on the entomopathogen.

Vegetative Growth and Phylogenetic Relationship of Commercially Cultivated Strains of Pleurotus eryngii based on ITS sequence and RAPD

Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at 30degrees C and minimum mycelial growth observed at 10degrees C. This mushroom tolerates a broad pH range for mycelial growth, with most favorable growth observed at pH 6. Results also indicated that glucose peptone, yeast malt extract and mushroom complete media were favorable growth media, while Hennerberg and Hoppkins media were unfavorable. Dextrin was the best and xylose the least effective carbon sources. Results revealed that inorganic nitrogen sources were less effective than organic sources for the mycelial growth of P. eryngii. Investigation of genetic diversity is necessary to identify the strains. The ITS region of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 214 to 222 bp and 145 to 236 bp, respectively. The sequence of ITS2 was more variable than that of ITS1, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into six clusters. Fourteen IUM and ATCC-90212 strains were also analyzed by RAPD with 20 arbitrary primers. Fourteen of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.2 to 2.3 kb.

Compatibilidade de isolados de Beauveria bassiana (Ascomycetes: Clavicipitaceae) com carrapaticidas químicos utilizados no controle do carrapato dos bovinos

The purpose of the present study was to assess compatibility between IBCB66 and IBCB21 isolates of Beauveria bassiana and acaricides: Flumethrin + Coumaphos, Deltamethrin, Dichlorvos + Cypermethrin, Dichlorvos + Chlorpyrifos, Cypermethrin High Cis, Dichlorvos + Cypermethrin High Cis, Cypermethrin and Amitraz, utilized on the control of Rhipicephalus (Boophilus) microplus in our country. The effect of commercial products on the isolates was assayed according to observation of vegetative growth, conidia production, and viability of strains of B. bassiana fungus. With concerning about IBCB66 isolate, products Deltamethrin, Cypermethrin High Cis and Amitraz were compatible, not affecting the entomopathogen development. Product Cypermethrin was toxic, and products Flumethrin + Coumaphos, Dichlorvos + Cypermethrin, Dichlorvos+ Chlorpyrifos and Dichlorvos + Cypermethrin High Cis were very toxic. In regard to IBCB21 isolate, products Flumethrin + Coumaphos, Dichlorvos + Cypermethrin, Dichlorvos + Chlorpyrifos, Cypermethrin High Cis, Dichlorvos + Cypermethrin High Cis and Cypermethrin were very toxic and product Amitraz was toxic. From the acaricides evaluated, product Deltamethrin was the single agent that did not produce toxic effect on the entomopathogen.

O presente trabalho teve como escopo avaliar a compatibilidade dos isolados IBCB66 e IBCB21 de Beauveria bassiana com os carrapaticidas a base de Flumetrina + Coumafós, Deltametrina, Diclorvós + Cipermetrina, Diclorvós + Clorpirifós, Cipermetrina High Cis, Diclorvós + Cipermetrina High Cis, Cipermetrina e Amitraz utilizados no controle do carrapato Rhipicephalus (Boophilus) microplus em nosso país. O efeito dos produtos comerciais sobre os isolados foi verificado por meio da observação do crescimento vegetativo, produção de conídios e viabilidade das estirpes do fungo B. bassiana. Com referência ao isolado IBCB66, os produtos Deltametrina, Cipermetrina High Cis e Amitraz foram classificados como compatíveis, não interferindo no desenvolvimento do entomopatógeno. O produto Cipermetrina foi classificado como tóxico e os produtos Flumetrina + Coumafós, Diclorvós + Cipermetrina, Diclorvós + Clorpirifós e Diclorvós + Cipermetrina High Cis, muito tóxicos. Em relação ao isolado IBCB21, os produtos Flumetrina + Coumafós, Diclorvós + Cipermetrina, Diclorvós + Clorpirifós, Cipermetrina High Cis, Diclorvós + Cipermetrina High Cis e Cipermetrina foram classificados como muito tóxicos e o produto Amitraz como tóxico. Entre os carrapaticidas avaliados, o produto Deltametrina foi o único que não produziu efeito tóxico sobre o entomopatógeno.

Desenvolvimento de cafeeiros enxertados 'Apoatã IAC 2258' cultivados em recipiente de 250 litros / Development of coffee trees (Coffea arabica L. L.) grafted in 'Apoatã IAC 2258' grown in recipient of 250 liters

Visando obter informações sobre o desenvolvimento de duas cultivares de cafeeiros Coffea arabica L. enxertados ou não em Coffea canephora Pierre, cultivar 'APOATÃ IAC 2258' foi instalado e conduzido esse ensaio, de janeiro de 2004 a maio de 2005. O delineamento experimental utilizado foi em blocos casualizados em esquema fatorial (2 x 3) + 2, com parcelas subdivididas no tempo e quatro repetições, sendo duas cultivares de Coffea arabica L., três tipos de mudas (enxertada, auto enxertada e pé franco), duas testemunhas do porta-enxerto Apoatã pé franco e auto enxertado e quatro épocas. Foram avaliadas altura de plantas, número de ramos plagiotrópicos, número de nós nos ramos plagiotrópicos, massa seca da parte aérea e massa seca do sistema radicular. Os resultados mostraram que a muda enxertada não é superior à muda de pé franco para nenhuma característica avaliada, independentemente da cultivar. Não foi observado efeito do estresse causado pela enxertia, mais sim efeito do porta enxerto Apoatã, para todas as características. As plantas de Apoatã apresentam maior acúmulo de massa seca de raiz que as plantas de C. arabica L.. Não se recomenda a utilização de mudas enxertadas para áreas isentas de nematóides.

This study was carried out during the period from January 2004 until May 2005, aimed to obtain information on the development of coffee trees grafted in 'APOATÃ IAC 2258' and ungrafted coffee trees. The experimental design used was a randomized block with a (2 x 3) + 2 factorial split-plot arrangement and four replications, as follows: two C. arabica L. cultivars, three types of seedling (grafted, self-grafted and ungrafted), two Apoatã rootstock control, ungrafted and self-grafted evaluated in four period of time. The experiments were evaluated for plant height, aerial parts dry matter, root system dry matter, plagiotropic shoots number and plagiotropic shoots nodes number. The results obtained let verify that grafted seedling was not superior to the ungrafted one for all the evaluated characteristics, regardless of the cultivar. No stress effect caused by grafting was observed, but Apoatã rootstock effect was observed for all features. Apoatã plants have more root system dry matter than C. arabica L. plants. The use of grafted seedling is not recommended for nematoid-free areas.

Vegetative Growth of Four Strains of Hericium erinaceus Collected from Different Habitats

Vegetative growth of four different strains of Hericium erinaceus was observed. The temperature suitable for optimal mycelial growth was determined to be 25degrees C, with growth observed in the extend temperature range of 20~30degrees C. The different strains of this mushroom showed distinct pH requirements for their optimum vegetative growth, with the most favorable growth observed at pH 6. Considering vegetative mycelial growth, PDA, YM, Hennerberg, Hamada, and Glucose peptone were the most favorable media, and Czapek Dox, Hoppkins, Glucose tryptone, and Lilly were the most unfavorable media for these mushroom strains. With the exception of lactose, most of the carbon sources assayed demonstrated favorable vegetative growth of H. erinaceus. For mycelial growth, the most suitable nitrogen source was alanine and the most unsuitable was histidine. Oak sawdust medium supplemented with 10~20% rice bran was the best for mycelial growth of the mushroom.