Occurrence and antimicrobial resistance of pathogenic vibrios isolated from green mussel, Perna viridis L. 1758 in Bacoor Bay, Cavite, Philippines

OBJECTIVES: To determine the occurrence and antimicrobial resistance of pathogenic vibrios from green mussel, Perna viridis in bacoor bay cavite. METHODS: Vibrios were isolated using TCBS agar and confirmed using API Identification kit. The relationship between the density of vibrios and environment parameters such as months, harvest sites and other physico-chemical parameters of water was determined. The isolated vibrios were subjected to antibiotic resistance testing to determine the resistance patterns.RESULTS: Pathogenic vibrios were isolated in all of the 90 mussel samples. Of the 183 vibrio isolates, 75 strains were V. alginolyticus and 53 strains belonged to V. cholerae wherein 18 (34%) of these were V. cholerae serotype O1. Forty nine (49) strains belonged to V. parahaemolyticus and six were V. vulnificus. Using the regression analysis, salinity, water temperature and ph have significant correlation on the density of vibrios (p=0.0309), with regression model, total vibrios = -16990763 + 145858 Salinity + 186808 Temperature + 1037886 pH. In the antimicrobial resistance assay,ampicillin had the highest drug resistance (37.8%) followed by nalidixic acid (10.4%), tetracycline (10.4%) and co-trimoxazole (9.3%).CONCLUSION: Pathogenic vibrios were isolated in mussel of Bacoor, Cavite and can be predicted using salinity, temperature and pH of the water. Thirty six percent (36%) of Vibrio isolates were resistance to ampicillin.

Carga bacteriana de los peces Cynoscion squamipinnis (Perciformes: Scianidae) y Lutjanus gutattus (Perciformes: Lutjanidae) en la cadena de comercialización, Costa Rica

Bacteriological load of the fishes Cynoscion squamipinnis and Lutjanus gutattus in the marketing chain, Costa Rica. To determine the bacteriological quality of fishery products in the different stages from commercialization, monthly samples were taken during March 2004 and February 2006 from a Costa Rica marketing chain. Microbiological analyses were made to determine total coliforms (CT), faecal coliforms (CF), Escherichia coli (EC), aerobic total count (RTA), Salmonella sp., Staphylococcus aureus, Vibrio cholerae and Vibrio parahaemolyticus. Three body zones were analyzed: skin, belly and muscle. There were differences in the amount of CT between parts: skin had the highest counts, 11% of samples were identified as E. coli. and 2.5% of total counts were higher than the legal limit. Only 1.3% of the samples were S. aureus-positive. Salmonella sp., V. cholerae and V. parahaemolyticus were absent. Rev. Biol. Trop. 57 (1-2): 45-52. Epub 2009 June 30.

Con el fin de determinar la calidad bacteriológica de los productos pesqueros en las diferentes etapas de comercialización, desde marzo del 2004 y hasta febrero del 2006, recolectamos muestras mensuales y realizamos análisis microbiológicos para determinar coliformes totales (CT), coliformes fecales (CF), Escherichia coli, recuento total aerobio (RTA), Salmonella sp., Staphylococcus aureus, Vibrio cholerae y Vibrio parahaemolyticus en la piel, el vientre y la carne de los productos. Hubo diferencias en la cantidad de CT encontrada entre las diferentes zonas corporales muestreadas, siendo la piel la zona con mayor conteo. En el 11% de las muestras identificamos E. coli y el 2.5% de los recuentos totales superaron los límites máximos aceptados. Salmonella sp., V. cholerae y V. parahaemolyticus estuvieron ausentes, mientras que sólo el 1.3% de las muestras fueron positivas para S. aureus.

Detección de Vibrio cholerae No 01 en algunos ambientes acuáticos de Colombia / Detection Vibrio cholerae No 01 in some aquatic environment of Colombia

Para detectar Vibrio cholerae spp. ambiental en algunos de las zonas de Colombia donde tuvo lughar la epidemia de 1991 a 1993, se tomaron muestras de agua y se procesaron de acuerdo con los métodos recomendados. A los aislados resultante se les practicaron pruebas bioquímicas, de crecimiento y de susceptibilidad a antibióticos. De las 11 muestras de agua, se seleccionaron de manera preliminar 690 calonias cuyas caracteríticas correspondían a Vibrio Cholerae. La selección definitiva se obtuvieron 4 aislamientos de Vibrio cholerae no 01, provenientes de Tumaco. Estos aislados resultaron resistentes a la mayoría de antbióticos utilizados, no productores de toxina ni con genes que codificaran tal función. La presencia de estos aislados no 01 puede indicar que se están constituyendo reservorios acúaticos, lo cual es un indicador de que el cólera en Colombia está pasando a una fase de endemia.

Enumeração e identificação de víbrios sacarose-positivos em amostras de lulas frescas comercializadas no município de Niterói-RJ / Enumeration and identification of sucrose-positive vibrios in samples of fresh squids commercialized at Niterói county-RJ

Foi efetuado um estudo sobre a enumeração e a identificação de víbrios sacarose-positivos em lulas frescas obtidas no comércio varejista do município de Niterói-RJ. No experimento, em 50 amostras de lulas, identificadas como pertencentes à espécie Oorytheutis brasiliensis Blainville, 1823, foram isoladas Unidades Formadoras de Colônias (UFC) sacarose-positivas em 28 (56%) amostras. Destas, foram identificados o Vibrio a/ginolyticus e víbrios do grupo NAG (não aglutináveis). A média dos Números Mais Prováveis (NMP) dos víbrios totais (com exceção da amostra com NMP > 2400 bacts./g) foi 101,70 bacts./g. Do total de 29 isolamentos nas 28 amostras, o V alginolyticus do grupo I de Heiberg obteve o maior percentual (62,07%), o V alginolyticus do grupo 111 apenas 10,30% e os víbrios NAG com 27,63%.

From 50 samples of squids of lhe species Dorytheutis brasiliensis Blainville, 1823, Colonies Formed Units (CFUs) sucrose-positives Víbrio alginolyticus and NAG vibrios (non-agglutinable) were isolated. The average of the Most Probabble Number of total vibrios (except the sample with MPN > 2400 bacts./g) was 101,70 bacts./g. From 28 samples, 29 isolations were carried out and lhe V. alginolyticus group I of Heiberg's Classification showed the greatest percentage (62,07%). The V. alginolyticus group 111 showed only 10,30% and NAG vibrios 27,63%.

Enumeração e identificação de Vibrio parahaemolyticus em lulas frescas comercializadas no município de Niterói RJ Brasil / Enumeration and identification of Vibrio parahaemolyticus in fresh squids commercialized at Niterói, Rio de Janeiro State, Brazil

Com o objetivo de verificar as condições higiênico-sanitárias do pescado comercializado no município de Niterói, efetuou-se um estudo sobre a enumeração e identificação de Vibrio parahaemolyticus em lulas frescas. Foram utilizadas 50 amostras de lulas frescas, identificadas como pertencentes à espécie Oorytheutis brasiliensís Blainville, 1823. A identificação e enumeração do V. parahaemolyticus foram baseadas nos métodos descritos pela lnternational Commission on Microbiological Specifications for Foods - ICMSF (1983). A classificação de Heiberg (1936) foi utilizada como teste complementar. O V. parahaemolyticus foi identificado em três (6%) amostras, sendo que, em uma o vibrio se desenvolveu em meio com concentração salina a 10%, na prova de halofilismo. O V. parahaemolyticus apresentou os NMPs (Número Mais Provável) com média de 19,3.bactérias/g. As três amostras de V. parahaemolyticus submetidas à classificação de Heiberg apresentaram perfil compatível com o grupo VIl (Sacarose -, Manose + e Arabinose +). Concluiu-se que, mesmo sob comercialização inadequada, com manipulação e resfriamento precários, as amostras analisadas apresentaram o V. parahaemolyticus com frequências e NMPs baixos.

A study on the enumeration and identification of Vibrio parahaemolyticus in freshsquids in Niterói was undertook aiming at checking the hygienic-sanitary conditions of fish commercialized in that City. Fifty fresh squids (Oorytheutis brasiliensis, Blainville, 1823). Methods described by the lnternational Comission on Microbiological Specification for Foods-ICMSF (1983) were used to identify and enumerate V. parahaemoiyticus and Heiberg's classi-fication was a complementai test. v. parahaemoiyticus was identified in three samples (6%); in one of them it grew in an environment with 10% salt concentration during halophilism probe. Most probable number (MPN) average for V. parahaemoiyticus was 19,3 bacteria per gram. The three samples submitted to Heiberg's classification showed a profile compatible with group VIl (sacarose-, manose+, arabinose-). We concluded that, even under inadequate commercialization, manipulation and refrigeration conditions, V. parahaemolyticus frequencies and MPNs are low.