Binding of the anti-inflammatory drug niflumic acid to bovine serum albumin

Binding of the anti-inflammatory drug niflumic acid to serum albumin was measured by equilibrium dialysis and the dissociation constants were determined. The maximal binding capacity was 36 mol niflumic acid per mol albumin. Most of the binding sites were of low affinity, only six having dissociation constants below 1 mM. At the plsma concentrations most frequently used in eperimental work, the high affinity sites account for more than 99% of the albumin-bound influmic acid