RESUMEN
Gastric (GC) and breast (BrC) cancer are two of the most common and deadly tumours. Different lines of evidence suggest a possible causative role of viral infections for both GC and BrC. Wide genome sequencing (WGS) technologies allow searching for viral agents in tissues of patients with cancer. These technologies have already contributed to establish virus-cancer associations as well as to discovery new tumour viruses. The objective of this study was to document possible associations of viral infection with GC and BrC in Mexican patients. In order to gain idea about cost effective conditions of experimental sequencing, we first carried out an in silico simulation of WGS. The next-generation-platform IlluminaGallx was then used to sequence GC and BrC tumour samples. While we did not find viral sequences in tissues from BrC patients, multiple reads matching Epstein-Barr virus (EBV) sequences were found in GC tissues. An end-point polymerase chain reaction confirmed an enrichment of EBV sequences in one of the GC samples sequenced, validating the next-generation sequencing-bioinformatics pipeline.
Asunto(s)
Femenino , Humanos , Masculino , Neoplasias de la Mama/virología , ADN Viral/aislamiento & purificación , /genética , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , ARN Viral/aislamiento & purificación , Neoplasias Gástricas/virología , Computadores , Biología Computacional/métodos , Simulación por Computador/economía , Análisis Costo-Beneficio/métodos , México , Ácidos Nucleicos/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Análisis de Secuencia de ADN/métodos , Análisis de Secuencia de ARN/métodosRESUMEN
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7 percent) followed by Leptospira spp. in 4 cases (3.2 percent),bovine herpesvirus (BHV) and bovine viral diarrhea (BVDV) in 3 animals (2.4 percent) each, and 1 for the association of BVDV and BHV. In 77.4 percent (96/124) of the samples it was not possible to detect any agent.