RESUMEN
No abstract available.
Asunto(s)
Anciano , Humanos , Masculino , Antibacterianos/farmacología , Cefotaxima/análogos & derivados , Colangiopancreatografia Retrógrada Endoscópica , Cálculos Biliares/cirugía , Bacterias Anaerobias Gramnegativas/efectos de los fármacos , Infecciones por Bacterias Gramnegativas/diagnóstico , Metronidazol/uso terapéutico , Pruebas de Sensibilidad Microbiana , ARN Ribosómico 16S/química , Análisis de Secuencia de ADN , Tomografía Computarizada por Rayos XRESUMEN
ABSTRACT This article presents a case of tick infestation of the lower eyelid by a previously unreported species. A 71-year-old male presented with a tick attached to the lower eyelid. The tick was identified morphologically, and then molecularly via polymerase chain reaction (PCR) and sequencing of its DNA. In addition, a review of the literature relevant to the genera of ticks associated with infestation of the human eye is provided. The tick, which was in the nymphal developmental stage, was first identified according to taxonomic keys as Dermacentor sp. For complete species identification, 16s rDNA gene PCR and sequencing were performed, which showed that the tick was D. marginatus. Systematizing tick species could assist physicians in determining the potential for transmission of tick-borne human diseases.
RESUMO Este artigo apresenta um caso de infestação por carrapatos da pálpebra inferior por uma espécie previamente não declarada. Um homem de 71 anos de idade apresentou-se com um carrapato grudado na pálpebra inferior. O carrapato foi identificado morfologicamente, e, em seguida, uma estrutura molecular através de reacção em cadeia da polimerase (PCR) e a sequenciação do seu DNA. Além disso, uma análise da literatura pertinente aos gêneros de carrapatos associados à infestação do olho humano é fornecido. O carrapato, que estava em fase de desenvolvimento das ninfas, foi identificado pela primeira vez de acordo com chaves taxonômicas com o Dermacentor sp. Para identificação de espécies completa, gene 16S rDNA PCR e sequenciamento foram realizadas, que mostrou que o carrapato foi D. marginatus. Sistematizando espécie de carrapato poderia ajudar os médicos a determinar o potencial de transmissão de doenças humanas transmitidas por carrapatos.
Asunto(s)
Humanos , Masculino , Anciano , Infestaciones por Garrapatas/parasitología , Garrapatas/clasificación , Garrapatas/genética , Garrapatas/parasitología , Infecciones Parasitarias del Ojo , Párpados/parasitología , Filogenia , ADN/aislamiento & purificación , ADN/química , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/química , Reacción en Cadena de la Polimerasa , Enfermedades de los Párpados/parasitología , Conformación de Ácido NucleicoRESUMEN
BACKGROUND: Next-generation sequencing (NGS) can detect many more microorganisms of a microbiome than traditional methods. This study aimed to analyze the vaginal microbiomes of Korean women by using NGS that included bacteria and other microorganisms. The NGS results were compared with the results of other assays, and NGS was evaluated for its feasibility for predicting vaginitis. METHODS: In total, 89 vaginal swab specimens were collected. Microscopic examinations of Gram staining and microbiological cultures were conducted on 67 specimens. NGS was performed with GS junior system on all of the vaginal specimens for the 16S rRNA, internal transcribed spacer (ITS), and Tvk genes to detect bacteria, fungi, and Trichomonas vaginalis. In addition, DNA probe assays of the Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis were performed. Various predictors of diversity that were obtained from the NGS data were analyzed to predict vaginitis. RESULTS: ITS sequences were obtained in most of the specimens (56.2%). The compositions of the intermediate and vaginitis Nugent score groups were similar to each other but differed from the composition of the normal score group. The fraction of the Lactobacillus spp. showed the highest area under the curve value (0.8559) in ROC curve analysis. The NGS and DNA probe assay results showed good agreement (range, 86.2-89.7%). CONCLUSIONS: Fungi as well as bacteria should be considered for the investigation of vaginal microbiome. The intermediate and vaginitis Nugent score groups were indistinguishable in NGS. NGS is a promising diagnostic tool of the vaginal microbiome and vaginitis, although some problems need to be resolved.
Asunto(s)
Femenino , Humanos , Área Bajo la Curva , Bacterias/genética , Proteínas Bacterianas/genética , Candida/genética , Proteínas Fúngicas/genética , Gardnerella vaginalis/genética , Secuenciación de Nucleótidos de Alto Rendimiento , Microbiota , ARN Ribosómico 16S/química , Curva ROC , Análisis de Secuencia de ADN , Trichomonas vaginalis/genética , Vagina/microbiología , Vaginitis/diagnósticoRESUMEN
BACKGROUND: Acinetobacter baumannii has a greater clinical impact and exhibits higher antimicrobial resistance rates than the non-baumannii Acinetobacter species. Therefore, the correct identification of Acinetobacter species is clinically important. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has recently become the method of choice for identifying bacterial species. The purpose of this study was to evaluate the ability of MALDI-TOF MS (Bruker Daltonics GmbH, Germany) in combination with an improved database to identify various Acinetobacter species. METHODS: A total of 729 Acinetobacter clinical isolates were investigated, including 447 A. baumannii, 146 A. nosocomialis, 78 A. pittii, 18 A. ursingii, 9 A. bereziniae, 9 A. soli, 4 A. johnsonii, 4 A. radioresistens, 3 A. gyllenbergii, 3 A. haemolyticus, 2 A. lwoffii, 2 A. junii, 2 A. venetianus, and 2 A. genomospecies 14TU. After 212 isolates were tested with the default Bruker database, the profiles of 63 additional Acinetobacter strains were added to the default database, and 517 isolates from 32 hospitals were assayed for validation. All strains in this study were confirmed by rpoB sequencing. RESULTS: The addition of the 63 Acinetobacter strains' profiles to the default Bruker database increased the overall concordance rate between MALDI-TOF MS and rpoB sequencing from 69.8% (148/212) to 100.0% (517/517). Moreover, after library modification, all previously mismatched 64 Acinetobacter strains were correctly identified. CONCLUSIONS: MALDI-TOF MS enables the prompt and accurate identification of clinically significant Acinetobacter species when used with the improved database.
Asunto(s)
Humanos , Infecciones por Acinetobacter/microbiología , Acinetobacter baumannii/química , Proteínas Bacterianas/química , Bases de Datos Factuales , Filogenia , ARN Ribosómico 16S/química , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
No abstract available.
Asunto(s)
Adulto , Anciano , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Biopsia , Colon/microbiología , Fusobacterium/genética , Enfermedades Inflamatorias del Intestino/microbiología , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/química , República de Corea , Análisis de Secuencia de ADNRESUMEN
No abstract available.
Asunto(s)
Animales , Perros , Femenino , Humanos , Persona de Mediana Edad , Antibacterianos/farmacología , Mordeduras y Picaduras , Pruebas Antimicrobianas de Difusión por Disco , Pasteurella/efectos de los fármacos , Infecciones por Pasteurella/diagnóstico , ARN Ribosómico 16S/química , República de Corea , Análisis de Secuencia de ADN , Infecciones de los Tejidos Blandos/diagnósticoRESUMEN
Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Brucker Biotyper (Brucker Daltonics, Bremen, Germany) and molecular typing with 16S rRNA gene sequencing confirmed the 6 isolates as S. pettenkoferi. The conventional phenotypic identification of these isolates is not reliable owing to their inconsistent biochemical characteristics. Five of the 6 isolates were found to be resistant to oxacillin, and all isolates showed susceptibility to vancomycin and linezolid. For accurate identification of this novel species, advanced methods by using Brucker Biotyper or molecular methods such as 16S rRNA gene sequencing are required.
Asunto(s)
Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Antibacterianos/farmacología , ADN Bacteriano/química , Farmacorresistencia Bacteriana/efectos de los fármacos , Linezolid/farmacología , Pruebas de Sensibilidad Microbiana , Oxacilina/farmacología , Fenotipo , ARN Ribosómico 16S/química , Análisis de Secuencia de ADN , Infecciones Estafilocócicas/tratamiento farmacológico , Staphylococcus/efectos de los fármacos , Vancomicina/farmacologíaRESUMEN
No abstract available.
Asunto(s)
Anciano , Humanos , Masculino , Pueblo Asiatico , Enterobacteriaceae/clasificación , Filogenia , Neumonía/diagnóstico , Enfermedad Pulmonar Obstructiva Crónica/diagnóstico , ARN Ribosómico 16S/química , República de Corea , Análisis de Secuencia de ADNRESUMEN
No abstract available.
Asunto(s)
Anciano , Humanos , Masculino , Bronquiectasia/diagnóstico , Mycobacterium/clasificación , Infecciones por Mycobacterium/diagnóstico , Filogenia , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/química , República de Corea , Análisis de Secuencia de ADNRESUMEN
No abstract available.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Pueblo Asiatico , Infecciones por Flavobacteriaceae , Flavobacterium/genética , Cirrosis Hepática Alcohólica/sangre , ARN Ribosómico 16S/química , República de Corea , Análisis de Secuencia de ADNRESUMEN
BACKGROUND: Microbiological laboratories seek technologically innovative solutions to cope with large numbers of samples and limited personnel and financial resources. One platform that has recently become available is the Kiestra Total Laboratory Automation (TLA) system (BD Kiestra B.V., the Netherlands). This fully automated sample processing system, equipped with digital imaging technology, allows superior detection of microbial growth. Combining this approach with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) (Bruker Daltonik, Germany) is expected to enable more rapid identification of pathogens. METHODS: Early growth detection by digital imaging using Kiestra TLA combined with MS was compared to conventional methods (CM) of detection. Accuracy and time taken for microbial identification were evaluated for the two methods in 219 clinical blood culture isolates. The possible clinical impact of earlier microbial identification was assessed according to antibiotic treatment prescription. RESULTS: Pathogen identification using Kiestra TLA combined with MS resulted in a 30.6 hr time gain per isolate compared to CM. Pathogens were successfully identified in 98.4% (249/253) of all tested isolates. Early microbial identification without susceptibility testing led to an adjustment of antibiotic regimen in 12% (24/200) of patients. CONCLUSIONS: The requisite 24 hr incubation time for microbial pathogens to reach sufficient growth for susceptibility testing and identification would be shortened by the implementation of Kiestra TLA in combination with MS, compared to the use of CM. Not only can this method optimize workflow and reduce costs, but it can allow potentially life-saving switches in antibiotic regimen to be initiated sooner.
Asunto(s)
Humanos , Automatización de Laboratorios , Candida albicans/genética , Pruebas Antimicrobianas de Difusión por Disco , Bacterias Gramnegativas/genética , Bacterias Grampositivas/genética , ARN Ribosómico 16S/química , Estudios Retrospectivos , Análisis de Secuencia de ARN , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
No abstract available.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Secuencia de Bases , Carnobacteriaceae/genética , Bases de Datos Genéticas , Infecciones por Bacterias Grampositivas/sangre , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/química , Análisis de Secuencia de ARNRESUMEN
No abstract available.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Actinobacteria/genética , Bacteriemia/diagnóstico , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/química , República de Corea , Análisis de Secuencia de ARN , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
No abstract available.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Pueblo Asiatico , Bacteriemia/complicaciones , Secuencia de Bases , Diabetes Mellitus Tipo 2/complicaciones , Pie Diabético/microbiología , Cocos Grampositivos/genética , ARN Ribosómico 16S/química , República de CoreaRESUMEN
No abstract available.
Asunto(s)
Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Recién Nacido , Masculino , Persona de Mediana Edad , Antibacterianos/farmacología , Ceftriaxona/farmacología , Ecocardiografía , Endocarditis Bacteriana/diagnóstico , Inmunosupresores/uso terapéutico , Pruebas de Sensibilidad Microbiana , Micrococcaceae/efectos de los fármacos , ARN Ribosómico 16S/química , República de CoreaRESUMEN
No abstract available.
Asunto(s)
Femenino , Humanos , Persona de Mediana Edad , Antibacterianos/farmacología , ADN Bacteriano/análisis , Hipertrofia/diagnóstico , Imagen por Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Mycobacterium/clasificación , Micobacterias no Tuberculosas/clasificación , Filogenia , ARN Ribosómico 16S/química , Reacción en Cadena en Tiempo Real de la Polimerasa , República de Corea , Análisis de Secuencia de ARN , Homología de Secuencia de Ácido Nucleico , Tenosinovitis/diagnósticoRESUMEN
No abstract available.
Asunto(s)
Adulto , Humanos , Masculino , Antibacterianos/farmacología , Trasplante de Médula Ósea , Capnocytophaga/efectos de los fármacos , Infecciones por Bacterias Gramnegativas/complicaciones , Leucemia Linfocítica Crónica de Células B/complicaciones , Pruebas de Sensibilidad Microbiana , Ácido Penicilánico/análogos & derivados , Piperacilina/farmacología , ARN Ribosómico 16S/química , Análisis de Secuencia de ARN , Homología de Secuencia de Ácido Nucleico , Trasplante Homólogo , Resultado del TratamientoRESUMEN
No abstract available.
Asunto(s)
Anciano , Humanos , Masculino , Antibacterianos/uso terapéutico , Bursitis/diagnóstico , Cefoperazona/uso terapéutico , Diabetes Mellitus Tipo 2/complicaciones , Nocardia/genética , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/química , Análisis de Secuencia de ARN , Sulbactam/uso terapéuticoRESUMEN
No abstract available.
Asunto(s)
Anciano , Humanos , Masculino , Ampicilina/uso terapéutico , Antibacterianos/uso terapéutico , Antimetabolitos Antineoplásicos/uso terapéutico , Pueblo Asiatico , Citarabina/uso terapéutico , Quimioterapia Combinada , Idarrubicina/uso terapéutico , Leucemia Mieloide Aguda/complicaciones , Moraxella/genética , Infecciones por Moraxellaceae/diagnóstico , ARN Ribosómico 16S/química , República de Corea , Infecciones del Sistema Respiratorio/diagnóstico , Análisis de Secuencia de ARN , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Sulbactam/uso terapéuticoRESUMEN
Clostridium difficile, an anaerobic, spore-forming, gram-positive, rod-shaped bacterium, is the most common nosocomial pathogen causing pseudomembranous colitis. C. difficile is not intrinsically invasive and rarely infects extraintestinal sites. The bacterium, therefore, is not commonly detected in blood cultures. Here, we report a case of C. difficile bacteremia in a patient who had underwent loop ileostomy because of rectal obstruction following metastatic colon cancer originated from prostate cancer.