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1.
The Korean Journal of Parasitology ; : 341-347, 2011.
Artículo en Inglés | WPRIM | ID: wpr-78173

RESUMEN

Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of Acanthamoeba, we compared the transcription profile between cysts and trophozoites using microarray analysis. The DNA chip was composed of 12,544 genes based on expressed sequence tag (EST) from an Acanthamoeba ESTs database (DB) constructed in our laboratory, genetic information of Acanthamoeba from TBest DB, and all of Acanthamoeba related genes registered in the NCBI. Microarray analysis indicated that 701 genes showed higher expression than 2 folds in cysts than in trophozoites, and 859 genes were less expressed in cysts than in trophozoites. The results of real-time PCR analysis of randomly selected 9 genes of which expression was increased during cyst formation were coincided well with the microarray results. Eukaryotic orthologous groups (KOG) analysis showed an increment in T article (signal transduction mechanisms) and O article (posttranslational modification, protein turnover, and chaperones) whereas significant decrement of C article (energy production and conversion) during cyst formation. Especially, cystein proteinases showed high expression changes (282 folds) with significant increases in real-time PCR, suggesting a pivotal role of this proteinase in the cyst formation of Acanthamoeba. The present study provides important clues for the identification and characterization of encystation mediating factors of Acanthamoeba.


Asunto(s)
Animales , Acanthamoeba castellanii/genética , Análisis por Conglomerados , Bases de Datos Genéticas , Etiquetas de Secuencia Expresada , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica/genética , Análisis de Secuencia por Matrices de Oligonucleótidos , Oocistos/fisiología , Proteínas Protozoarias/genética , ARN Protozoario/genética , Trofozoítos/fisiología
2.
The Korean Journal of Parasitology ; : 283-285, 2007.
Artículo en Inglés | WPRIM | ID: wpr-114844

RESUMEN

To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba.


Asunto(s)
Animales , Acanthamoeba castellanii/genética , Secuencia de Aminoácidos , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Estadios del Ciclo de Vida , Datos de Secuencia Molecular , Proteínas Protozoarias/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Regulación hacia Arriba
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