Trueperella pyogenes como causa de aborto em uma vaca - relato de caso / Trueperella pyogenes as an abortion cause in a cow - case report

Trueperella pyogenes é uma bactéria oportunista que causa mastite, metrite e abortos esporádicos em bovinos. Este trabalho relata um caso incomum de abort em uma vaca por Trueperella pyogenes. Um feto bovino, fêmea, mestiço Brahman, com oito meses de gestação, foi encaminhado para exame anatomopatológico e exames complementares. Na necropsia, evidenciou-se grande quantidade de líquido serossanguinolento e moderada quantidade de fibrina recobrindo a pleura visceral e o saco pericárdico. Os pulmões estavam difusamente avermelhados e consolidados, com áreas firmes esbranquiçadas ao corte de não mais de 1cm. No exame histopatológico, observou-se pneumonia necrossupurativa, pleurite fibrinopurulenta e placentite purulenta. No exame microbiológico, isolou-se T. pyogenes nas amostras de fígado, pulmões, conteúdo abomasal do feto e placenta. O feto foi negativo na PCR para Neospora caninum, Toxoplasma gondii e vírus da diarreia viral bovina (BVDV). Trueperella pyogenes geralmente causa broncopneumonia supurativa com formação de abscessos, porém, no presente feto abortado, observaram-se lesões macro e microscópicas comumente descritas em casos de aborto por Brucella abortus. Este estudo constata, então, a importância dessa bactéria como causa de aborto em bovinos, com lesões semelhantes à brucelose, destacando sua relevância dentro das causas de aborto em bovinos e o potencial zoonótico pouco explorado.(AU)

Trueperella pyogenes is an opportunistic bacterium associated with mastitis, metritis and occasional abortion in bovines. Here we report an uncommon case of abortion by T. pyogenes in a cow. An aborted female Brahman bovine fetus, at 8 months of gestational age was submitted for anatomopathological examination and complementary diagnostic tests. Macroscopic findings at necropsy included large amounts of free serum-blood fluid and moderate fibrin deposition covering both the visceral pleura and pericardial sack. The lungs were diffusely reddened and markedly consolidated, showing widespread smaller than 1cm, hard, white nodules. Necrosuppurative pneumonia, fibrinopurulent pleuritis, and purulent placentitis were the main histopathologic alterations observed. Trueperella pyogenes was isolated from liver, lungs, abomasa contents and placental samples. All tissue samples were PCR-negative for Neospora caninum, Toxoplasma gondii and bovine viral diarrhea virus (BVDV). Although T. pyogenes is often involved in suppurative bronchopneumonia and abscesses formation, macro and microscopic lesions in the present report were compatible with those commonly attributed to Brucella abortus fetal infections. Trueperella pyogenes is an important bovine pathogen with a neglected zoonotic potential being responsible for infections that can mimic other diseases' typical presentations.(AU)

Herida abscesificada debido a Trueperella bernardiae / Abscessed wound resulting from Trueperella Bernardiae

Trueperella bernardiae es un microorganismo grampositivo facultativo anaerobio que forma parte de la microbiota normal de la piel y de la orofaringe, que recientemente ha sido reasignado l género Trueperella. Este patógeno ha sido descrito en muy pocos casos como causante de infección en los seres humanos, debido a su aspecto corineforme y su presencia en cultivos mixtos, y a las dificultades diagnósticas

Use of MALDI-TOF MS in diagnostic microbiology for identification of species that conventional methods usually fail to identify

(MALDI-TOF MS) has been used in clinical diagnostic laboratories for the identification of microorganisms. It has a relevant advantage compared to other methods in terms of speed to provide results, being an alternative for addressing restrictions in clinical diagnosis as it may replace or complement existing identification techniques. This is especially important because some rare microorganisms would be identified only by higher cost techniques which are not widely available, such as genetic sequencing. Thus, the present paper reports two cases in which uncommon microorganisms were identified effectively and quickly. (AU)

Cervicofacial Actinomycosis with Orbit Involvement

Actinomycosis is caused by filamentous Gram positive anaerobic bacteria from the Actinomycetaceae family, and known as a rare cause of the infection at the eyeball. We report magnetic resonance findings of a 60-year-old Korean man with cervicofacial actinomycosis, including cellulitis in the eye and central nervous system actinomycosis. On orbital magnetic resonance imaging, gadolinium-enhanced T1-weight images showed multiple abnormal enhancing lesions in head and neck including right eye, and some include low signal intensities which considered as abscesses. The lesions was diagnosed as actinomycosis by incisional biopsy, and since then was cured by using antibiotics of penicillin family.

Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing / 国际口腔科学杂志·英文版

The objective of this study was to investigate the compositional profiles and microbial shifts of oral microbiota during head-and-neck radiotherapy. Bioinformatic analysis based on 16S rRNA gene pyrosequencing was performed to assess the diversity and variation of oral microbiota of irradiated patients. Eight patients with head and neck cancers were involved in this study. For each patient, supragingival plaque samples were collected at seven time points before and during radiotherapy. A total of 147,232 qualified sequences were obtained through pyrosequencing and bioinformatic analysis, representing 3,460 species level operational taxonomic units (OTUs) and 140 genus level taxa. Temporal variations were observed across different time points and supported by cluster analysis based on weighted UniFrac metrics. Moreover, the low evenness of oral microbial communities in relative abundance was revealed by Lorenz curves. This study contributed to a better understanding of the detailed characterization of oral bacterial diversity of irradiated patients.

Assessment of feather hydrolysate from thermophilic actinomycetes for soil amendment and biological control application

Protein-rich hydrolysate from feather waste was obtained using a mixed culture of selected thermophilic actinomycete strains, and was tested for possible application as soil amendment and biological control agent. For this purpose, a 4 months laboratory experiment was carried out using two types of urban soils [Sofia, Bulgaria]: native park soil and anthropogenic soil. The effect of the obtained hydrolysate on some soil parameters [pH, some enzyme activities and microbial activity], seed germination and ryegrass growth, and activity against some plant pathogenic fungi was studied. The results demonstrated that soil enrichment with the organic solution in low concentrations exerted a positive effect on soil urease and microbial activity, seed germination and ryegrass growth, and this trend was better expressed in the anthropogenic soils. Feather hydrolysate showed good activity against plant pathogenic fungi Fusarium solani, Fusarium oxysporum, Mucor sp. and Aspergillus niger. Produced antifungal compounds were isolated and partially characterized as amphiphilic peptides. To the best of our knowledge, antifungaLpeptides produced by Thermoactinomyces sp. have not been reported. Therefore, the feather hydrolysate obtained by means of the mixed culture of Thermoactinomyces strains has potential to be used as alternative organic amendment for restoration of contaminated soils and for accelerating ryegrass growth. It could successfully used also for as biocontrol agent applicable to crop plant soil

Soft tissue infections with Arcanobacterium haemolyticum: report of three cases.

We report here three polymicrobial wound infections associated with Arcanobacterium haemolyticum in rural patients aged between 60-65 years. In two patients, one with cellulitis and the other with postoperative wound infection following amputation of the limb, Arcanobacterium haemolyticum was isolated repeatedly along with beta haemolytic streptococci (BHS). The BHS belonged to Lancefield's group G and group C respectively. In another patient, who was a diabetic with chronic osteomyelitis, Arcanobacterium haemolyticum was isolated along with Proteus vulgaris . All the three isolates of Arcanobacterium haemolyticum isolated by us were uniformly resistant to cotrimoxazole and sensitive to penicillin, erythromycin, clindamycin, ciprofloxacin and gentamicin. Erythromycin alone or combined therapy of penicillin with erythromycin or penicillin with ciprofloxacin was effective in treating these infections.

Respiratory tract infection due to Arcanobacterium haemolyticum--a case report.

A case of respiratory tract infection due to Arcanobacterium haemolyticum is reported. A female of 26 years presented with cough with expectoration and fever off and on for a duration of six weeks. Mycobacterium tuberculosis and Arcanobacterium haemolyticum were isolated. Arcanobacterium haemolyticum was isolated on three separate occasions.

Septic arthritis due to Arcanobacterium haemolyticum.

Diphtheroids or "coryneform" bacilli are usually considered to be nonpathogenic "normal flora" of human skin and mucous membranes. Because bacterial cultures are frequently contaminated with these organisms the correct diagnosis and treatment may be delayed by the failure to recognize serious infections caused by them. Few confirmed cases of orthopaedic infections due to Arcanobacterium haemolyticum infection have been reported, partly because of inadequate identification of this bacterium. We report a case of septic arthritis due to A. haemolyticum.

Isolamento de Arcanobacterium pyogenes de granuloma actinomicóide em bovino / Isolation of Arcanobacterium pyogenes of actinomycotic-like granuloma in a cow

Foi observada em um bovino com suspeita de lechiguana, uma lesäo arredondada de consistência dura na regiäo sub-mandibular que media 12x11x7cm. Foram realizadas biópsias cirúrgicas para estudos bacteriológico e histológico, teste de tuberculinizaçäo intra-dérmico e coleta de sangue, a fim de realizar o teste de imunodifusäo radial para o diagnóstico de lechiguana. Posteriormente, foi realizada eutanásia e necropsia do animal. No resultado das biópsias, näo se obteve isolamento bacteriano e o estudo histológico näo foi conclusivo. Os testes de tuberculinizaçäo e de imunodifusäo apresentaram resultado negativo. Na necropsia, constatou-se que a lesäo consistia de um nódulo, formado por uma cápsula de tecido conjuntivo envolvendo uma massa esbranquiçada com grânulos amarelados de até 3mm de diâmetro. Através de histologia, constataram-se piogranulomas multifocais, com um infiltrado de neutrófilos circundando rosetas formadas por colônias bacterianas no centro e corpúsculos de Splendore-Hoeppli na periferia; ao redor desse infiltrado purulento observaram-se células epitelióides e tecido fibroso infiltrado por linfócitos. Arcanobacterium pyogenes foi isolado em cultura pura do material obtido na necropsia. Conclui-se que A. pyogenes poderia ter participaçäo na etiologia de granuloma com características clínicas e patológicas semelhantes à lechiguana, tuberculose e actinobacilose.

Sinusitis debida a Arcanobacterium haemolyticum / Arcanobacterium haemolyticum sinusitis

Arcanobacterium haemolyticum es un patógeno bacteriano que afecta principalmente a adolescentes y adultos jóvenes. Rara vez causa infección severa. Se presenta un caso de sinusitis en una paciente de 18 años de edad, del sexo femenino, con rash cutáneo, lo que ocurre en más de la mitad de los casos de faringitis. La cepa resultó sensible a eritromicina.

Antifungal activity (invitro) of certain polyene macrolide antibiotics against various plant pathogens.

Certain polyene macrolides were developed from different actinomycetes at Research and Development of Hindustan Antibiotics Limited. These antibiotics were screened for the antifungal activity against various plant pathogens. IC50 and MIC of each of the antibiotic against the plant pathogens was found out and is being reported.

Production of free amino acids by some earthworm-borne microorganisms.

Production of free amino acids by some earthworm-borne microorganisms was investigated in three different synthetic media. Among the fungi tried Gliocladium roseum and Heterocephallum aurantiacum; among bacteria screened Bacillus macerans and B. mycoides; and among actinomycetes tested Streptomyces rimosus, S. violans, S. antibiticus, S. corchorusii and S. atroolivaceus produced significant amount of free amino acids. No correlations could be observed between vegetative growth and free amino acid production.

Pulmonary Nocardiosis Diagnosed by Fine Needle Aspiration: A Case Report / 대한세포병리학회지

Nocardia, aerobic members of the order of Actinomycetaceae, produces infections in human lung. Nocardial infection is associated with underlying diseases of immuno-suppression or treatment with corticosteroid. It is difficult to detect Nocardia by sputum examination or histologic sections and it has rarely been diagnosed by fine needle aspiration of the lung. We describe a case of pulmonary nocardiosis in a 72 year-old man. diagnosed by fine needle aspiration, which was confirmed by culture of aspirates. The aspirates showed neutrophil-predominant inflammatory cells with microoganisms demonstrated by Gomori methenamine silver and Gram stain. The organisms had characteristic long branching filamentous strucutres. The lesions on chest x-ray were in resolution with antimicrobial therapy.

Studies on the keratinolytic enzymes of thermophilic actinomycetes.1-Production of thermostable keratinase enzyme and identification of keratinolytic thermophilic actinomycetes

One hundred and ninety thermophilic actinomycetes were isolated from different sources. Screening was carried out according to growth temperatures. Obligate thermophilic actinomycetes were picked up and examined according to their keratinolytic activity. Results indicated that five isolates have keratinase activity. The two most active keratinolytic actinomycete isolates C52 and G114 were selected and subjected to complete identification. The cultural, morphological and physiological characteristics of both isolates indicated that they belong to Theinoactinomyces vulgaris and Microbispora thermodiastatica. The effect of nutritional and environmental conditions on the growth and keratinase activity of Th. vulgaris C52 were studied. Data revealed that Th. vulgaris C52 grew better with good yield of keratinase activity by growing it on the modified Kosmatchev medium [50 ml medium/500 ml clinical bottle], in which the nitrogen and the carbon sources of the Kosmatchev medium were replaced with 1.6% [W/V] sterile chicken feather pieces and 1% [W/V] starch, using tap water with an initial pH of 7.5, inoculated with 2% [v/v] of homogenized spore suspension [containing approximately 7.5 x 10 4 spores ml-1] of one day old culture and incubated at 50§C for 4 days under static condition