RESUMEN
Biological tissue adhesive using ammonium sulfate method was prepared from human blood and the bonding power of this glue was demonstrated by gluing 1 cm[2] pieces of guinea pigs skin and human bone at 10 and 30 minutes after gluing. Fracturing of the incudomallear complex in 24 guinea pigs was done then the complex was reunited using either own blood clot in control animals or the biological ammonium sulfate tissue adhesive in the experimental animals. All the animals were examined after one week, two weeks and four weeks macro scopically and histologically. After four weeks complete healing by new bone formation was noticed in the experimental group in comparison to the control group that showed fibrous tissue healing. The self prepared fibrin adhesive provides bonding power adequate for middle ear surgery, eliminates transmission of viral diseases and appears promising as a relatively safe biological [autogenous] material for otologic surgery; and is worthy for further study