Analysis of essential oil in herbal pair Artemisia annua-Agastache rugosa by GC-MS and chemometric resolution method / 药学学报

Gas chromatograph-mass spectrometry, chemometric resolution method (CRM) and overall volume integration method were used to analyze the essential components of herbal pair Artemisia annua-Agastache rugosa (AA-AR) and compare it with that of single herbs AA and AR. The results showed that the components of volatile oil of herbal pair (AA-AR) were different from that of single herb drug in quality and quantity. 70, 69, and 48 essential components in essential oil of herbal pair (AA-AR), AA and AR were determined, accounting for about 85.93%, 88.85% and 93.23% of the total volatile oil, respectively. The volatile active components of the essential oils compounds in number are almost the sum of that of two single herbs, are mainly from herb AA, and the contents of each component from herb AR were relatively high. There are 51 common active constituents shared by herbal pair AA-AR and AA, and 34 common active constituents shared by herbal pair AA-AR and AR. There are 7 new components in the essential oils of herbal pair AA-AR, the relative content of arteannuic acid (2.99%) and p-propenyl-anisole (1.92%) are higher than others.

Textual research on history of introduction corrected and herbal medicine of Pogostemon cablin / 中国中药杂志

<p><b>OBJECTIVE</b>To study the history of introduction and herbal medicine of Pogostemon cablin by textual researches and clarify the confusion in literatures.</p><p><b>METHOD</b>Textual research on historical literatures, practical applications and field investigation were adopted.</p><p><b>RESULT</b>The varieties of P. cablin for therapeutic uses were not chaotic, however there has existed confusion between P. cablin and Agastache rugosus in descriptions of appellation, habitat and characteristic of the original plants.</p><p><b>CONCLUSION</b>It was proved that the term "Huoxiang" in Chinese medicinal works before Ming Dynasty was pointed to P. cablin. The cultivation history of P. cablin in China was traced to Liang Dynasty or before.</p>

Anti-fungal test of composite agastache lotion on seven pathogenic fungi and its clinical application / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effect of Chinese composite recipe in treating mycotic infection.</p><p><b>METHODS</b>The growth condition of 7 kinds of fungi cultured on the media containing composite agastache lotion (CAL, consisted of 5 Chinese drugs) of different concentration was observed. Result showed that CAL could inhibit 7 kinds of fungi. Based on the above anti-fungus test, 110 patients with skin tinea or genital candidiasis were treated separately with CAL, western medicine and combined (CAL and western) medicines, the therapeutic effects of the 3 groups were observed and compared.</p><p><b>RESULTS</b>The therapeutic effect in patients treated with combined medicine was significantly better than that in the other two groups (P < 0.05).</p><p><b>CONCLUSION</b>Combined use of CAL and western medicine could enhance the cure rate in treating skin tinea and genital candidiasis. Attention should be paid on studying Chinese anti-fungal agents.</p>

DNA sequencing and molecular identification of Patchouli and its substitute wrinkled gianthyssop / 药学学报

<p><b>AIM</b>To analyze sequences of the nuclear ribosomal RNA small subunit (18S rRNA) gene and the chloroplast matK gene of crude drug Patchouli (Pogostemon cablin) in order to provide molecular evidence for identification of Patchouli drug.</p><p><b>METHODS</b>To sequence the entire 18S rRNA gene and partial matK gene of Patchouli from Guangzhou and its substitute Wrinkled Gianthyssop (Agastache rugosa) from Sichuan using PCR direct sequencing and to detect the homology of two gene sequences between these two crude drugs.</p><p><b>RESULTS</b>The complete 18S rRNA gene sequence is 1,805 bp in length for Patchouli from Guangzhou whereas 1,794 bp for Wrinkled Gianthyssop from Sichuan. The 3'-end sequence of matK gene is 521 bp (747-1,268 nt from upstream of matK gene) for these two crude drugs. Based on multiple sequence alignment, it is found that there are 18 variable sites and 11 aligned gap sites in 18S rRNA sequence, 49 variable sites in 3'-matK sequence between these two crude drugs. The homology is 98.4% for 18S rRNA and 90.6% for 3'-matK between two crude drugs, respectively.</p><p><b>CONCLUSION</b>DNA sequencing can provide an accurate and reliable tool in the crude drug identification of Patchouli and its substitute Wrinkled Gianthyssop.</p>