Study of distribution and metabolism of methamphetamine in hair of guinea pig / 法医学杂志

OBJECTIVE@#To study the distribution and metabolism of methamphetamine in the hair of guinea pig.@*METHODS@#Determination of methamphetamine and its metabolite amphetamine in hair was performed by GC/MS and GC/NPD. Concentration-time course of methamphetamine and amphetamine in hair of guinea were recorded. Relationship between hair color, administrated dose and drug concentration in hair were also discussed.@*RESULTS@#The concentration of amphetamine is higher than the concentration of methamphetamine in the hair of guinea administrated a single dose or seven doses of methamphetamine. The concentration of methamphetamine and amphetamine were significantly related with administration dose and the incorporation rate into white and brown hair is much poorer than that of black hair.@*CONCLUSION@#Administration methods, dose and the color of hair affect the concentration of methamphetamine and amphetamine.

Role of methamphetamine metabolism in the development of CNS tolerance the drug / Rol del metabolismo de la metanfetamina en el desarrollo de tolerancia del sistema nervioso central a los efectos de la droga

We have previously observed that pretreatment with increasing doses of methamphetamine (METH) atteniates the effects of METH on the dopaminergic and serotonergic systems as compared with the ones observed in nonpretreated controls. In order to understand the mechanism of this tolerance, untreated rats pretreated with METH (daoly doses of 5.0, 7.5; and 10 mg/kg, s.c., at 6h intervals with a 24h drug-free period between each dose) were challenged with the administration of 5 doses of METH (15 mg/kg, s.c., at 6h intervals). The metabolism of METH in brain, liver and blood was studied measuring the concentration of METH and its metabolites 2,4,6,8 and 10h after the last dose by gas chromatography/mass spectrometry techniques. The forebrain concentrations of METH in the pretreated animals were significanthy lower than those observed in the forebrain of naive animals. Liver concentrations of METH in the pretreated animals were not significantly modified as compared to the ones of naive animals, but in liver amphetamine and the p-hydroxylated metabolites, p-hydroxyamphetamine (p-OH-AMP) and p-hydroxymethamphetamine (p-OH-METH), were significantly greater than those observed in the naive group. Bloo levels of METH, AMP and their p-hydroxylated metabolites were also greater in the pretreated animals. The involvement of an altered distribution of methamphetamine in the CNS and the development of tolerance is discussed

A dehydrogenase system to demonstrate oxidation of amphetamine and related compounds by rat brain homogenate.

Amphetamine, ephedrine, mescaline and chlorpromazine act as substrate for dehydrogenase system of rat brain homogenate. This system appears quite different from the usual deaminating process of these compounds, which required NADP and oxygen. The present system proceeds more rapidly in anaerobic condition.