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1.
Journal of Veterinary Science ; : 363-371, 2011.
Artículo en Inglés | WPRIM | ID: wpr-17400

RESUMEN

The nucleotide sequence of the VP1 (1D) and partial 3D polymerase (3Dpol) coding regions of the foot and mouth disease virus (FMDV) vaccine strain A/Iran87, a highly passaged isolate (~150 passages), was determined and aligned with previously published FMDV serotype A sequences. Overall analysis of the amino acid substitutions revealed that the partial 3Dpol coding region contained four amino acid alterations. Amino acid sequence comparison of the VP1 coding region of the field isolates revealed deletions in the highly passaged Iranian isolate (A/Iran87). The prominent G-H loop of the FMDV VP1 protein contains the conserved arginine-glycine-aspartic acid (RGD) tripeptide, which is a well-known ligand for a specific cell surface integrin. Despite losing the RGD sequence of the VP1 protein and an Asp26-->Glu substitution in a beta sheet located within a small groove of the 3Dpol protein, the virus grew in BHK 21 suspension cell cultures. Since this strain has been used as a vaccine strain, it may be inferred that the RGD deletion has no critical role in virus attachment to the cell during the initiation of infection. It is probable that this FMDV subtype can utilize other pathways for cell attachment.


Asunto(s)
Secuencia de Aminoácidos , Sustitución de Aminoácidos , Antígenos Virales/química , Proteínas de la Cápside/química , Clonación Molecular , Virus de la Fiebre Aftosa/clasificación , Regulación Viral de la Expresión Génica , Datos de Secuencia Molecular , Filogenia , Proteínas no Estructurales Virales/química
2.
Rev. chil. infectol ; 25(supl.1): S14-S18, abr. 2008.
Artículo en Español | LILACS | ID: lil-480661

RESUMEN

Rabies glycoprotein is the only exposed protein which is inserted in the viral lipidie envelope. This 65-67 kda protein is a N-glycosilated transmembrane protein forming trimers on the viral surface. It has been identified as the major pathogenicity determinant, playing a role in the budding, viral axonal transport during infection, apoptosis and immune evasion. It is also the major antigen responsible for the protective immune response and it is been used in commercial recombinant vaccines. Its structure, antigenicity and pathogenic role have been well studied, identifying main antigenic sites that have the responsibility for virulence, cellular receptors attachment and epitope acquisition.


La glicoproteína del virus rábico es la única proteína viral expuesta, encontrándose inserta en la envoltura lipídica. Esta molécula de 65-67 kda corresponde a una proteína trans-membrana N-glicosilada que se dispone en forma de trímeros en la superficie viral. Ha sido identificada como el mayor determinante de pato-genicidad, participando además en procesos de yemación, flujo axonal del virion durante la infección, apoptosis y evasión de la respuesta inmune. Es también el principal antígeno inductor de la respuesta inmune protectora siendo utilizado en vacunas recom-binantes comerciales. Su estructura, antigenicidad e implicancias en la patogenia han sido bien estudiadas identificándose los principales sitios antigénicos responsables de la patogenicidad, unión a receptores celulares y formación de epitopos.


Asunto(s)
Animales , Humanos , Antígenos Virales , Glicoproteínas , Virus de la Rabia/patogenicidad , Proteínas del Envoltorio Viral , Antígenos Virales/química , Antígenos Virales/inmunología , Antígenos Virales/fisiología , Glicoproteínas/química , Glicoproteínas/inmunología , Glicoproteínas/fisiología , Conformación Proteica , Virus de la Rabia/inmunología , Virus de la Rabia/metabolismo , Virulencia , Proteínas del Envoltorio Viral/química , Proteínas del Envoltorio Viral/inmunología , Proteínas del Envoltorio Viral/fisiología
3.
Journal of Veterinary Science ; : 57-65, 2003.
Artículo en Inglés | WPRIM | ID: wpr-122777

RESUMEN

The N protein of the rinderpest virus (RPV) was analyzed topologically and antigenically by using anti-N monoclonal antibodies (Mabs). Ten Mabs were raised against the N protein of the RPV. At least six non-overlapping antigenic sites (sites A-F) were delineated by competitive binding assays using biotinylated Mabs. Of them 5 sites (A, C, D, E and F) on the N protein were recognized by RPV-specific Mabs in ELISA and IFA while site B was recognized by Mabs reacting with both RPV and PPRV. Non- reciprocal competition was found among sites C, D and E. Recombinant RPV N protein after exposure to 0.2% SDS exhibited higher ELISA titers in all Mabs recognizing 6 sites. Four sites (A, B, E and F) on 2% SDS-treated N protein lost completely reactivity with Mabs while the remaining sites (C and D) on the protein retained their antigenicity to some degree. It indicates that two sites (C and D) were sequential. Six representative Mabs bound to each site exhibited competition with rinderpest antibodies in a blocking ELISA, indicating that the sites were actively involved in antigenicity in cattle.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Antígenos Virales/química , Unión Competitiva , Ensayo de Inmunoadsorción Enzimática , Epítopos/inmunología , Proteínas de la Nucleocápside/química , Virus de la Peste Bovina/inmunología
4.
J Postgrad Med ; 1990 Oct; 36(4): 185-90
Artículo en Inglés | IMSEAR | ID: sea-117140

RESUMEN

One hundred and twelve cases of cervical pathology (58 paraffin sections and 54 cervical smears) were assessed by PAP technique and 30 cases by indirect immunofluorescent technique. Forty-two normal cervical smears from the age matched controls were stained by indirect immuno-fluorescent technique. HSV-2 antigen was detected by PAP method in 86 out of 112 cases (78.57%) i.e. 50/55 squamous cell carcinoma, 13/13 carcinoma in situ, 11/15 severe dysplasia, 3/4 moderate dysplasia and 9/16 mild dysplasia. The amount of antigen was maximum in squamous cell carcinoma and decreased in carcinoma in situ, severe, moderate and mild dysplasia in descending order. Three cases of adenocarcinoma cervix were negative. Only one case out of 42 controls was positive. HSV-2 antigen was detected by an indirect IF technique in 8/9 squamous cell carcinoma, 2/3 carcinoma in situ, 3/7 dysplasia, O/1 adenocarcinoma and 4/10 inflammatory cases. The above findings support the association between HSV-2 and squamous cell carcinoma cervix, as well as carcinoma in situ which is statistically significant.


Asunto(s)
Adulto , Antígenos Virales/química , Femenino , Técnica del Anticuerpo Fluorescente , Herpes Simple/inmunología , Humanos , Inmunohistoquímica , Incidencia , India/epidemiología , Persona de Mediana Edad , Estudios Prospectivos , Estudios Retrospectivos , Displasia del Cuello del Útero/química , Neoplasias del Cuello Uterino/química , Frotis Vaginal
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