Adaptação acadêmica em universitários / Academic adaptation in university students / Adaptación académica en estudiantes universitários

O objetivo do estudo foi investigar o impacto das variáveis habilidades sociais, resolução de problemas sociais, automonitoria, autoeficácia e coping na adaptação acadêmica em estudantes de instituições de ensino superior públicas e privadas. Participaram 637 estudantes de ambos os sexos, sendo 36,5% (115) homens de instituições públicas e 22,3% (72) de instituições privadas, com idade variando entre 18 e 38 anos (M=24,7; DP=6,3), de diferentes graduações. Foram utilizados o Inventário de Resolução de Problemas Sociais, o Inventário de Habilidades Sociais, a Escala de Automonitoria, a Escala de Autoeficácia Acadêmica de Estudantes do Ensino Superior, o Inventário de Estratégias de Coping e o Questionário de Vivências Acadêmicas-reduzido. A autoeficácia na gestão acadêmica (40,9%) e a autoafirmação na expressão de afeto positivo (13,7%) apresentaram maior impacto para os estudantes de instituições públicas e privadas, podendo contribuir com possíveis intervenções no processo de adaptação ao ensino superior. Pesquisas prospectivas podem investigar questões relacionadas a dados sociodemográficos.(AU)

The aim of the study was to investigate the impact of the variables Social Skills, Resolution of Social Problems, Self-monitoring, Self-efficacy and Coping on Academic Adaptation in students from public and private higher education institutions. 637 students of both sexes participated, being 36.5% (115) men from public institutions and 22.3% (72) from private institutions, aged between 18 to 38 years (M = 24.7; SD = 6.3), of different grades. The Social Problem Solving Inventory, the Social Skills Inventory, the Self-Monitring Scale, the Higher Education Students' Academic Self-Efficacy Scale, the Coping Strategies Inventory and the Academic Experiences-Reduced Questionnaire were used. Self-efficacy in Academic Management (40.9%) and Self-affirmation in the Expression of Positive Affection (13.7%) had a greater impact on students from public and private institutions, which may contribute to possible interventions in the process of adapting to Higher Education. Prospective research can investigate issues related to sociodemographic data.(AU)

El objetivo del estudio fue investigar el impacto de las variables Habilidades sociales, Resolución de problemas sociales, Autocontrol, Autoeficacia y Afrontamiento en la adaptación académica en estudiantes de instituciones de educación superior públicas y privadas. Participaron 637 estudiantes de ambos sexos, siendo 36,5% (115) hombres de instituciones públicas y 22,3% (72) de instituciones privadas, con edades entre 18 y 38 años (M = 24,7; SD = 6,3), de diferentes grados. Se utilizaron el Inventario de Resolución de Problemas Sociales, el Inventario de Habilidades Sociales, la Escala de Autocontrol, la Escala de Autoeficacia Académica de los Estudiantes de Educación Superior, el Inventario de Estrategias de Afrontamiento y el Cuestionario de Experiencias Académicas Reducidas. La Autoeficacia en la Gestión Académica (40,9%) y la Autoafirmación en la Expresión de Afecto Positivo (13,7%) tuvieron un mayor impacto en los estudiantes de instituciones públicas y privadas, lo que puede contribuir a posibles intervenciones en el proceso de adaptación a la Educación Superior. La investigación prospectiva puede investigar cuestiones relacionadas con los datos sociodemográficos.(AU)

Network pharmacology-based analysis of Chinese herbal Naodesheng formula for application to Alzheimer's disease / 中国天然药物

Naodesheng (NDS) formula, which consists of Rhizoma Chuanxiong, Lobed Kudzuvine, Carthamus tinctorius, Radix Notoginseng, and Crataegus pinnatifida, is widely applied for the treatment of cardio/cerebrovascular ischemic diseases, ischemic stroke, and sequelae of cerebral hemorrhage, etc. At present, the studies on NDS formula for Alzheimer's disease (AD) only focus on single component of this prescription, and there is no report about the synergistic mechanism of the constituents in NDS formula for the potential treatment of dementia. Therefore, the present study aimed to predict the potential targets and uncover the mechanisms of NDS formula for the treatment of AD. Firstly, we collected the constituents in NDS formula and key targets toward AD. Then, drug-likeness, oral bioavailability, and blood-brain barrier permeability were evaluated to find drug-like and lead-like constituents for treatment of central nervous system diseases. By combining the advantages of machine learning, molecular docking, and pharmacophore mapping, we attempted to predict the targets of constituents and find potential multi-target compounds from NDS formula. Finally, we built constituent-target network, constituent-target-target network and target-biological pathway network to study the network pharmacology of the constituents in NDS formula. To the best of our knowledge, this represented the first to study the mechanism of NDS formula for potential efficacy for AD treatment by means of the virtual screening and network pharmacology methods.

Network pharmacology-based analysis of Chinese herbal Naodesheng formula for application to Alzheimer's disease / 中国天然药物

Naodesheng (NDS) formula, which consists of Rhizoma Chuanxiong, Lobed Kudzuvine, Carthamus tinctorius, Radix Notoginseng, and Crataegus pinnatifida, is widely applied for the treatment of cardio/cerebrovascular ischemic diseases, ischemic stroke, and sequelae of cerebral hemorrhage, etc. At present, the studies on NDS formula for Alzheimer's disease (AD) only focus on single component of this prescription, and there is no report about the synergistic mechanism of the constituents in NDS formula for the potential treatment of dementia. Therefore, the present study aimed to predict the potential targets and uncover the mechanisms of NDS formula for the treatment of AD. Firstly, we collected the constituents in NDS formula and key targets toward AD. Then, drug-likeness, oral bioavailability, and blood-brain barrier permeability were evaluated to find drug-like and lead-like constituents for treatment of central nervous system diseases. By combining the advantages of machine learning, molecular docking, and pharmacophore mapping, we attempted to predict the targets of constituents and find potential multi-target compounds from NDS formula. Finally, we built constituent-target network, constituent-target-target network and target-biological pathway network to study the network pharmacology of the constituents in NDS formula. To the best of our knowledge, this represented the first to study the mechanism of NDS formula for potential efficacy for AD treatment by means of the virtual screening and network pharmacology methods.

De quando pensei sobre a escrita da psicanálise e me lembrei da música Oriente / When I thought of psychoanalytic writing and I remembered the song "Oriente"

O autor argumenta que o trabalho clínico psicanalítico é resultante da estruturação de um objeto psicanalítico reparador. Este objeto nada mais é do que a construção de uma metapsicologia pessoal, que é desenvolvida ao longo de todo o processo interminável de formação do psicanalista. Com base nessa metapsicologia pessoal, o psicanalista adquire a possibilidade de trabalhar no interior do círculo hermenêutico psicanalítico. O autor mostra a importância de uma análise para a construção desse objeto psicanalítico e o lugar que a escrita tem na consolidação do círculo hermenêutico psicanalítico. Suas observações são acompanhadas pela música "Oriente", de Gilberto Gil

The author argues that clinical-psychoanalytic work arises from structuring the restorative object (i.e., a psychoanalytic object that "repairs"). This object is nothing else besides the construction of a personal metapsychology, which is developed throughout the endless process of psychoanalytic training. This personal metapsychology enables the psychoanalyst to start working within the hermeneutic circle of psychoanalysis. The author shows the importance of analysis in order to construct this psychoanalytic object. He also emphasizes the role of psychoanalytic writing in strengthening the hermeneutic circle of Psychoanalysis. The author's observations come with the song "Oriente", composed by Gilberto Gil

El autor sostiene que el trabajo clínico psicoanalítico resulta de la estructuración de un objeto psicoanalítico reparador. Este objeto es la construcción de una metapsicología personal que se desarrolla a lo largo de todo el proceso interminable de formación psicoanalítica. A partir de esta metapsicología personal, el psicoanalista adquiere la posibilidad de trabajar dentro del círculo hermenéutico psicoanalítico. Sus observaciones son acompañadas por la música "Oriente" de Gilberto Gil

L'auteur soutient que le travail clinique psychanalytique provient de la structuration d'un objet psychanalytique réparateur. Cet objet n'est rien de plus que la construction d'une métapsychologie personnelle développée tout au long du processus interminable de formation du psychanalyste. A partir de cette métapsychologie personnelle, le psychanalyste a la possibilité de travailler au sein du cercle herméneutique psychanalytique. L'auteur démontre l'importance d'une analyse pour la construction de cet objet psychanalytique et la place de l'écriture dans la consolidation du cercle herméneutique psychanalytique. Ses observations s'accompagnent de la musique "Oriente", de Gilberto Gil

Personal journal blogs as manifest internal conversation toward self-innovation: a semiotic phenomenological analysis / Conversa interna manifesta como autoinovação nos diários pessoais em blogs: uma análise fenomenológico semiótica

This work is a semiotic phenomenological approach of writing in personal journal blogs to define and verify their potential as continuous and sustained movement toward personal change or self-innovation. The analysis juxtaposes, by quantitative and qualitative methods, chronological self-referential text data (semiotics) with the meaning of internal conversation disclosed in discourse (phenomenology). We take a specific blog domain for posts from random bloggers that would present self-referent terms as "I feel", "I think", "I believe", "my life", etc. Then we selected those posts with dense self-descriptions and expression of disparate personal thoughts and feelings. Approximately 150 posts were analyzed, resulting in a final sample of 12 homogeneous posts from a heterogeneous group of bloggers, nine females and three males, all native English speakers. We conclude that personal blog texts help convey psychological well-being through dialogical self-clarification, facilitate the emergence of new perspectives or self-actualizing, and can lead to self-innovation.

O presente trabalho recorre à fenomenologia semiótica para analisar diários pessoais em blogs com o objetivo de definir e verificar suas potencialidades como movimento contínuo e sustentado para mudanças pessoais e autoinovação. A análise justapõe, por métodos quanti e qualitativos, dados cronológicos e linguísticos de textos autorreferenciados (semiótica) com o sentido de conversas internas reveladas em discurso (fenomenologia). Foi tomado um domínio específico para postagens aleatórias de blogueiros que apresentassem termos autorreferentes, como "eu sinto" "eu penso", "eu acredito", "minha vida" etc. A partir disso, foram selecionadas postagens com autodescrições densas e díspares de pensamentos e sentimentos pessoais. Foram analisadas, aproximadamente, 150 postagens heterogêneas das quais retirou-se 12 postagens homogêneas, sendo 9 escritas por mulheres e 3 por homens, todos falantes nativos da língua inglesa. Concluiu-se que o texto de blog pessoal enseja bem-estar psicológico por meio de autoclarificações dialógicas, além de facilitar a emergência de novas perspectivas ou autoatualização podendo, assim, conduzir à autoinovação.

Esquecimentos, fantasias e sexualidade infantil: efeitos da autoanálise de Freud / Forgetfulness, fantasies and child sexuality: effects of Freud's self-analysis / El olvido, las fantasías y la sexualidad infantil: efectos del autoanálisis de Freud

Durante seu período de autoanálise, normalmente situado pelos estudiosos de sua obra entre os anos de 1897 e 1900, Freud descobriu, através da investigação de seus próprios sonhos e de suas lembranças infantis, os traços fundamentais de nosso inconsciente. Nesse artigo, nos propomos a desdobrar, através dos textos desse período, o processo e o contexto de descoberta que o levou a propor, dentre outros, o esquecimento, a fantasia e a sexualidade infantil como elementos estruturantes para o funcionamento do aparelho psíquico...

During his self-analysis period, usually located by the researchers of his work between the years 1897 and 1900, Freud discovered through the research of his own dreams and his childhood memories the fundamental features of our unconscious. In this article, we propose to investigate, through the texts of this period, the process and the context of discovery that led Freud to propose forgetting, fantasies and child sexuality as structural elements for the functioning of the psychic apparatus...

Durante su período de autoanálisis, que normalmente los estudiosos de su obra ubican entre los años 1897 y 1900, Freud descubrió las características fundamentales de nuestro inconsciente a través de la investigación de sus propios sueños y recuerdos de la infancia. En este artículo, proponemos a desarrollar, a través de los textos de la época, el proceso y el contexto de descubrimiento que le llevó a proponer, entre otros, el olvido, las fantasías y la sexualidad infantil como elementos estructurales para el funcionamiento del aparato psíquico...

O encontro com a saúde mental: uma perspectiva ético-estética do cuidado / The encounter with Mental Health: an ethical-aesthetic perspective of care

A formulação de uma nova caminhada para o cuidado em saúde mental está sendo estruturada com base na evidenciação do sujeito e na construção de um sistema que viabilize alternativas adequadas ao vivenciar humano na dimensão cidadã e que supere a lógica tradicional do diagnóstico e tratamento previamente estabelecido segundo abordagens expertises. Este é um desafio que está colocado para os trabalhadores da saúde que buscam respaldar suas práticas em teorias objetivantes e protocolos bem estabelecidos na procura pela segurança de se ter um papel definido e garantido. O risco que se corre nesta dimensão "segura" encontra-se na contramão que se insere em relação à necessidade do cuidado individual, sobretudo, no campo da Saúde Mental. Dentre os desafios colocados à rede de Atenção Psicossocial no cenário contemporâneo, este trabalho propôs potencializar a construção do conhecimento a partir do reconhecimento das singularidades dos usuários para complexificar o olhar sobre o cuidado através de um discurso menos reducionista da vida. A partir do problema disparador, esta pesquisa teve como objeto de estudo "o encontro com o usuário no âmbito da Atenção Psicossocial". Considera-se a noção de encontro pelo referencial da esquizoanálise como algo que é resultante da afetação dos corpos, em detrimento da indiferença. Os objetivos do estudo foram: Experimentar a pesquisa por meio da análise de implicações do pesquisador como legitimação dos afetos na produção de conhecimento; Avaliar a produção de cuidado a partir do regime ético-estético; Produzir sentidos para o encontro com a Saúde Mental a partir das singularidades dos usuários. Evitou-se enrijecer o processo da pesquisa; ao contrário, procurou-se caminhar junto com as mobilizações e potências, dificuldades, elaborações e propostas a cada momento. A partir da experimentação da pesquisadora no campo de pesquisa junto aos usuários do Centro de Atenção Psicossocial, o estudo trouxe a produção de dados sobre o cuidado a partir das produções dos encontros com os usuários. Propôs-se experimentar novos sentidos e olhares através da pesquisa em sua perspectiva estética, ressaltando-se a multiplicidade do ser que sente, pensa e age a partir das noções de diferença e singularidade, buscando recolher alguns efeitos dos encontros na pesquisa provocados nos participantes e em mim, a partir das interferências e dos encontros na pesquisa. O cerne das minhas considerações finais está no movimento, nos fluxos, e não em uma resposta com ponto final. Desta forma, não justifiquei ou legitimei neste estudo um conhecimento a respeito do cuidado, mas sim busquei (re)inventar, junto aos usuários co-pesquisadores uma noção de cuidado sob o prisma da diferença, da multiplicidade, e não da unificação ou totalização. Na produção dos dados emergiram intercessores para o encontro com a saúde mental e foram discutidas experimentações que apontaram para as ações da Rede de Atenção Psicossocial sob uma nova pensabilidade, a qual força a produção do cuidado em sua perspectiva imanente, em detrimento de perspectivas capitalísticas da totalização das formas do cuidado junto à pessoa portadora de transtorno psíquico grave. O tema da escuta foi algo que se produziu no estudo como algo que requer uma clínica menor para utilizarmos a plasticidade equilibrando-nos entre o extremo da surdez, o canto da sereia e o extremo da música gritante, para dar passagem às singularidades dos usuários. A partir dos dados produzidos na experimentação dos encontros no presente estudo, proponho para estudos futuros para a Saúde Mental uma "Produção do cuidado Órgão-sensível". Para superar a sobrecodificação das singularidades, devemos utilizar um movimento de produção de cuidado pela diferença: rizoma e não somente arborescência. Há que se compor a transversalidade rizoma-decalque nas ações da Atenção Psicossocial. O decalque instituído preservará os "órgãos" minimamente necessários. Mas, este "órgão" não será totalitário ou fascista se houver a composição com vertentes rizomáticas, as quais colocarão os "órgãos" em função da promoção da vida e não do organismo, à medida que são nômades, espontâneas, abertas à multiplicidade. Assim, aponta-se que há a necessidade de articularmos elementos da Saúde Mental com a perspectiva do cuidado pela diferença, e não apenas por referências teorizantes especulativas produtoras do achatamento produtivo-desejante dos encontros. Além disto, a partir das singularidades destacadas nos dados desta pesquisa, recomenda-se que estudos futuros adentrem também especificamente a ciência da enfermagem utilizando-se por dentro dela as noções de plasticidade e de rizoma como máquinas de guerra que adensem o tema da prática da enfermagem em saúde mental. A partir da convivência com estas pessoas que foram co-pesquisadoras, aponta-se, em algumas situações, que a loucura nem mesmo deva estar instituída obrigatoriamente como tema da psiquiatria, o que nos aponta a antipsiquiatria como um caminho possível e necessário para as reflexões na RAPS. Nesta perspectiva, a RAPS se desinstituir-se do poder que ainda possui e exerce na vida das pessoas ­ apesar da sociedade moderna ainda preferir que o louco esteja nos serviços e lugares instituídos especificamente para ele e que os demais modos de vida e saúde não pertencem a ele ­ significará para a RAPS a possibilidade de sustentar a proposta da desinstitucionalização da loucura: libertá-la

The formulation of a new pathway for mental health care is being structured based on the evidence of the subject and on the construction of a system that allows adequate alternatives to the human experience in the citizen dimension and that surpasses the traditional logic of diagnosis and treatment previously established according to approaches. This is a challenge for health workers who seek to support their practices in objectifying theories and well-established protocols in the search for the security of having a defined and guaranteed role. The risk that runs in this "safe" dimension is contrary to the need for individual care, especially in the field of Mental Health. Among the challenges posed to the network of Psychosocial Care in the contemporary scenario, this work proposed to potentialize the construction of knowledge from the recognition of the singularities of the users to complexify the look about care through a less reductionist discourse of life. From the triggering problem, this research had as object of study "the encounter with the user in the scope of Psychosocial Attention". The notion of encounter by the frame of schizoanalysis is considered as something that results from the affectation of the bodies, to the detriment of indifference. The objectives of the study were: To experiment the research by analyzing the implications of the researcher as legitimation of the affections in the production of knowledge; To evaluate the production of care from the ethical-aesthetic regime; To produce meanings for the encounter with Mental Health from the singularities of the users. The process of research was avoided; On the contrary, it was tried to walk along with the mobilizations and powers, difficulties, elaborations and proposals at each moment. Based on the researcher's experimentation in the field of research with users of the Center for Psychosocial Care, the study brought the production of data about care from the productions of encounters with users. It was proposed to experience new senses and looks through research in its aesthetic perspective, highlighting the multiplicity of the being that feels, thinks and acts from the notions of difference and singularity, seeking to gather some effects of the encounters in research provoked in the participants and in me, from the interferences and encounters in the research. The crux of my endings considerations is in the movement, in the flows, and not in a response with the final point. In this way, I did not justify or legitimize in this study a knowledge about care, but rather I sought to (re)invent, together with the co-researchers, a notion of care under the prism of difference, of multiplicity, not of unification or totalization. In the production of the data emerged intercessors for the encounter with mental health and were discussed experiments that pointed to the actions of the Network of Psychosocial Attention under a new pensabilidad, which forces the production of care in its immanent perspective, to the detriment of capitalist perspectives of the totaling the forms of care with the person with severe psychiatric disorder. The subject of listening was something that was produced in the study as something that requires a smaller clinic to use the plasticity balancing us between the extreme of the deafness, the siren's song and the extreme of the screaming music, to give way to the singularities of the users. From the data produced in the experimentation of the meetings in the present study, I propose for future studies for Mental Health a "Production of organ-sensitive care". To overcome the overcoding of the singularities, we must use a movement of production of care for difference: rhizome and not only arborescence. It is necessary to compose the rhizome-decal transversality in the actions of Psychosocial Attention. The decal instituted will preserve the "organs" minimally needed. But this "organ" will not be totalitarian or fascist if there is composition with rhizomatic slopes, which will place the "organs" in function of the promotion of life and not of the organism, as they are nomadic, spontaneous, open to multiplicity. Thus, it is pointed out that there is a need to articulate elements of Mental Health with the perspective of care for difference, and not only by speculative theorizing references producing the productive-desiring meeting of the meetings. In addition, from the singularities highlighted in the data of this research, it is recommended that future studies also specifically enter nursing science using the notions of plasticity and rhizoma as war machines that add to the theme of the practice of Nursing in mental health. From the coexistence with these people who were co-researchers, it is pointed out, in some situations, that madness should not even be established as a subject of psychiatry, which points us to antipsychiatry as a possible and necessary way for reflections in the Network of Psychosocial Attention. In this perspective, Network of Psychosocial Attention deinstitute itself from the power it still possesses and exerts in people's lives - although modern society still prefers that the insane person is in the services and places specifically instituted for him and that other ways of life and health do not belong to it - will mean for Network of Psychosocial Attention the possibility of sustaining the proposal of the deinstitutionalization of madness: to liberate it

O cuidado no hospital psiquiátrico sob a ótica da equipe de enfermagem / Care in psychiatric hospital under the perspective of a nursing team / Atención en hospital psiquiátrico bajo la perspectiva de enfermería del equipo

O presente estudo teve como objetivo descrever a percepção da equipe de enfermagem acerca do cuidado no hospital psiquiátrico. A pesquisa teve abordagem qualitativa, do tipo exploratória, com uso do grupo focal, com cinco participantes, em agosto de 2011, no município de Niterói, RJ, Brasil. A partir da análise dos dados, emergiram cinco categorias: escuta sensível; disponibilidade pessoal; projetos terapêuticos; fator humano da equipe; tensão “psiquiatria tradicional x paradigma psicossocial”. Concluiu-se que, apesar dos sujeitos da pesquisa atuarem ainda no modelo hospitalar, foi possível que trouxessem percepção do cuidado de forma humana e integral. Mas, esta percepção sobre o cuidado possuía fragilidade, pois não se evidenciaram as bases científicas da enfermagem. Recomenda-se que o profissional da equipe de enfermagem invista em seu papel no processo de cuidar no contexto da Reforma Psiquiátrica, em busca de uma abordagem centrada no sujeito e no seu modo de existir.

The present study is aimed at describing the perception of the nursing team concerning the care in a psychiatric hospital. The research used a qualitative approach, exploratory type, using focus group technique, with five participants, in August 2011, in Niteroi, RJ, Brazil. From the data analysis five categories emerged, covering: sensitive listening; personal availability; therapeutic projects; human issues of the team; Traditional Psychiatry vs. Psychosocial Paradigm tension. It was concluded that despite the research, the subjects were still working at the hospital model. It was possible to bring awareness in a human, comprehensive and complete manner. But this perception of care has frailties once it does not bring any evidence of scientific basis of nursing. It is recommended that the professional nursing team invest in their role of caring in the context of the Psychiatric Reform, in the pursuit of an approach centered on the subject and in his way of living.

El objetivo del presente estudio fue describir la percepción del personal de enfermería acerca de la atención en hospital psiquiátrico. Investigación con enfoque cualitativo, exploratorio, utilizando instrumentos de Análisis Institucional y de los grupos de enfoque con cinco participantes, en agosto de 2011, en Niterói, RJ, Brasil. A partir del análisis de datos, surgieroncinco categorías: escucha sensible; disponibilidad de personal; proyectos terapéuticos; factor humano de equipo; tensión “psiquiatría tradicional x paradigma psicosocial”. En conclusión, a pesar de los sujetos de investigación actuaren en el modelo hospitalario, fue posible traer percepción de cuidado humano e integral. Pero esta percepción poseía debilidad, porque no había evidencias de bases científicas de la enfermería. Se recomienda que el personal de enfermería invierta en su papel de cuidar en el contexto de la reforma psiquiátrica, en la búsqueda de enfoque centrado en el sujeto y su modo de existir.

Development of automatic urine monitoring system / 中国医疗器械杂志

An automatic urine monitoring system is presented to replace manual operation. The system is composed of the flow sensor, MSP430f149 single chip microcomputer, human-computer interaction module, LCD module, clock module and memory module. The signal of urine volume is captured when the urine flows through the flow sensor and then displayed on the LCD after data processing. The experiment results suggest that the design of the monitor provides a high stability, accurate measurement and good real-time, and meets the demand of the clinical application.

Influence of serum storage on the laboratory results of prostate-specific antigen / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the influence of serum storage on the laboratory results of serum T-PSA, F-PSA and FPSA%.</p><p><b>METHODS</b>Using automated chemiluminescence, we detected and compared the values of serum T-PSA, F-PSA and F-PSA% in the serum stored in different conditions.</p><p><b>RESULTS</b>When the serum was stored at 4 degrees C or at the room temperature (22 - 26 degrees C), FPSA was unstable as compared with T-PSA. Compared with the initial value, after 4 hours at the room temperature, F-PSA was decreased to (0.392 +/- 0.246) microg/L (P < 0.01), while T-PSA and F-PSA% to (1.522 +/- 1.085) microg/L and (25.03 +/- 5.94)%, respectively, with no significant difference; after 8 hours at the room temperature, T-PSA and F-PSA were reduced to (1.513 +/- 1.083) and (0.389 +/- 0.247) microg/L (P < 0.05 and P < 0.01). At 4 degrees C, T-PSA, F-PSA and F-PSA% were decreased to (9.418 +/- 7.965) microg/L, (2.168 +/- 1.558) micro/L and (26.6 +/- 6.63)%, respectively, after 2 days (P < 0.05), and to (9.203 +/- 7.736) microg/L, (2.047 +/- 1.478) microg/L and (25.64 +/- 6.56)% after 1 week (P < 0.01). At -40 degrees C, T-PSA, F-PSA and F-PSA% were (4.532 +/- 4.393) microg/L, (1.178 +/- 1.034) microg/L and (24.45 +/- 8.81)% after 4 weeks. When the serum was stored at -40 degrees C and after 3 freeze-thaws, F-PSA and T-PSA were (5.982 +/- 5.314) and (1.341 +/- 1.029) microg/L, respectively, with no significant difference from the initial values.</p><p><b>CONCLUSION</b>Different conditions of serum storage have different influences on the laboratory results of serum TPSA, F-PSA and F-PSA%, more on F-PSA than on T-PSA, while F-PSA% is relatively stable. At -40 degrees C, T-PSA and F-PSA may remain stable for a month at least. Repeated freeze-thaws of the serum do not affect the laboratory results of F-PSA and T-PSA.</p>

Assessment of the capability of the fluid model of automatic blood cell analyzer in white blood cell count and classification / 中华血液学杂志

<p><b>OBJECTIVE</b>To evaluate the application of XT-4000i automatic blood cell analyzer in white cell count and classification of cerebrospinal fluid (CSF).</p><p><b>METHODS</b>The fluid model of XT-4000i automatic blood cell analyzer was directly used to detect the white cell count and classification in 200 samples of CSF, and compared with the results obtained by manually microscopic counting method. White blood cell classification was performed by manual method under microscope with Wright's staining,and instrumental method with fluorescence staining and flow cytometry.</p><p><b>RESULTS</b>There is no statistical difference between instrumental and manual method in detecting the absolute counting of WBC, RBC, mononuclear cell and multinucleate cells (P>0.05), and there is a good correlation between the two methods (r=0.987, 0.999, 0.981 and 0.983 for WBC, RBC, mononuclear cell and multinucleate cell counts). Tumor cells in the samples with high fluorescent staining by instrumental method can be identified with Wright's staining by microscope method, which were consistent with the clinical diagnosis.</p><p><b>CONCLUSION</b>The fluid model of XT-4000i automatic blood cell analyzer was rapid and accurate in CSF white cell count and classification,and it also can provide preliminary information for tumor cells screening. The fluid model of XT-4000i automatic blood cell analyzer in white cell count and classification of CSF has the value of popularization in clinical application.</p>

Como Freud não conseguiu elaborar seu medo das datas fatídicas em sua autoanálise com Jung: [revisão] / Como Freud no logro elaborar su miedo a las fechas fatídica en su autoanálisis com Jung: [revisión] / On Freud’s inability to elaborate on his fear of fateful dates in his self-analysis with Jung: [review]

O ambiente cultural compartilhado pelo paciente e pelo analista pode desempenhar um papel facilitador no trabalho de transformação das moções pulsionais do id em representações de coisas inconscientes no paciente. A elaboração da crença de Freud em certas datas maléficas foi malsucedida em sua autoanálise com Jung por razões, entre outras, ligadas à contratransferência cultural deste. Teve sucesso, ao contrário, na autoanálise de Freud com Romain Rolland graças ao clima de conversa psicanalítica que se estabeleceu entre eles.

The cultural environment shared by patient and analyst can have a facilitating role in transforming the id's motion drives into the patient' unconcious representations of the things. Freud's elaboration of his belief that certain dates were evil or unlucky was unsuccessful in his self-analysis with Jung due to several reasons, including the latter's cultural countertransference. However, it succeeded in Freud's self-analysis with Romain Rolland, due to the atmosphere of psychoanalytic conversation established between them.

El ambiente cultural compartido por el paciente y por el analista puede desempeñar un papel facilitador en el trabajo de transformación de las mociones pulsionales del ello en representaciones de cosas inconscientes en el paciente. La elaboración de la creencia de Freud en determinadas fechas maléficas fue malograda en su autoanálisis con Jung por razones, entre otras, ligadas a la contratransferência cultural de este. Sin embargo, tuvo éxito en el autoanálisis de Freud con Romain Rolland gracias aI clima de conversación psicoanalítica que se estableció entre ellos.

Automated detection of malaria with haematology analyzer sysmex xe-2100.

Background: Diagnosis of malaria is usually made by microscopy [Giemsa, Acridine Orange (AO), and Quantitative Buffy Coat (QBC) assay], which requires expertise. Currently, automated haematology analyzers are being used for complete blood count (CBC), in all acute febrile and non-febrile illnesses which simultaneously detects malaria. The normal scattergram by the analyzer (Sysmex 2100) comprises of five parameters i.e. lymphocytes (pink), monocytes (green), neutrophils (blue), eosinophils (red) with a space between the neutrophil and eosinophil populations. Aims : We carried out a prospective study to compare the efficacy of Sysmex XE-2100 (Sysmex Corporation, Kobe) for detection of malaria in comparison to other conventional techniques. Materials and Methods : 430 cases were analyzed for malaria by microscopy (QBC, AO, Giemsa), ICT (Immunochromatography) and flowcytometric analyzer (Sysmex XE-2100). The abnormal scattergrams were observed as double neutrophil, double eosinophil, grey zone, extended neutrophil zone with a decrease space between eosinophil and neutrophil, and a combination of above patterns. Results : Out of 70 positive cases [49/70 (70%) P. vivax, 18/70 (25.7%) P. falciparum, and 3/70 (4.2%) both P. vivax and P. falciparum], 52 showed abnormal scattergrams by the analyzer. The sensitivity and specificity of hematology analyzer found to be 74.2% and 88%, respectively. Conclusion : Flowcytometric analyzer is a rapid, high throughput device which needs less expertization for the diagnosis of malaria. Hence, it can be used in the diagnostic laboratories as an early modality for diagnosis of malaria in suspected as well as clinically in apparent cases.

Cartografia dos processos educativos presentes no cotidiano de trabalho da equipe de enfermagem de um Hospital Psiquiátrico / Cartography of the educational processes present in the daily work of the nursing team of a Psychiatric Hospital

A presente pesquisa surgiu a partir da minha inquietação referente aos processos educativos permanentes junto à equipe de enfermagem que tem se desenhado no contexto do hospital psiquiátrico. A literatura do campo da enfermagem psiquiátrica, com foco no cuidado em saúde mental, descreve que a realidade da enfermagem ainda é incipiente no que diz respeito a compreender/modificar o processo de trabalho. O objeto de estudo foi a institucionalização da educação permanente da equipe de enfermagem no contexto de um hospital psiquiátrico. Essa dissertação teve como objetivo descrever os processos educativos permanentes para a equipe de enfermagem que ocorrem em um hospital psiquiátrico, identificando as lacunas e as potencialidades destes processos educativos para a equipe de enfermagem. Foi desenvolvida uma pesquisa de abordagem qualitativa, do tipo exploratório, com base no referencial teórico-metodológico da Análise Institucional. A coleta de dados foi realizada por meio de observação participante e de grupo focal. Os dados foram analisados com base na Análise Institucional e nos preceitos da Reforma Psiquiátrica. Apresenta-se como resultado a elaboração de uma cartografia a respeito dos processos educativos em enfermagem no cotidiano do hospital psiquiátrico. De acordo com as demandas da Reforma Psiquiátrica, o papel dos serviços de saúde deve ser o de garantir a visão do portador de sofrimento psíquico como cidadão, onde o tratamento não deve servir de tutela, mas sim de libertação. Para isso, é necessário que as equipes de enfermagem participem de espaços educativos onde possam refletir a respeito das novas práticas de cuidar no campo psicossocial, onde possam falar e ouvir sobre as implicações da Reforma Psiquiátrica para as equipes de enfermagem e onde possam refazer constantemente os atravessamentos individuais e coletivos dessas equipes sobre o indivíduo psicótico. Concluiu-se que a educação permanente em enfermagem deve ter como principal mote o desempenho de um cuidado como prática social. Deve propiciar que os profissionais de enfermagem busquem abordar o portador de sofrimento psíquico através da responsabilidade com o cuidado humano, com agenciamentos intra-equipe e extra-setor saúde, acompanhando esses portadores em seu dia a dia, isto é, na vida, respeitando-o em suas especificidades, em suas peculiares escolhas e apostando ­ ainda que provisoriamente ­ em vê-los usufruindo de um convívio social. Assim, as ações educativas em serviço devem servir para desenvolver novas formas de compreender e interpretar a realidade, questionar, discordar, propor soluções, favorecer a reflexão em favor da sociedade

The theme of this research came from the inquietude caused by the work as nurse and refers to the educative processes developed with the nursing team in the context of the psychiatric hospital. The literature in the psychiatric nursing field, with focus on the care in mental health, describes that the reality of nursing is still beginner as regards to understand/modify the work process. The object of study was the institutionalization of the permanent education of the nursing team in the context of the psychiatric hospital. This dissertation aimed to describe the permanent educative processes for the nursing team that occur in a psychiatric hospital, identifying the gaps and the potentialities of these educative processes for the nursing team. A qualitative and exploratory research was developed on the basis of the theoretical-methodological framework of the institutional analysis. The data collection was performed by participant observation and focus group. The data were analyzed on the basis of the institutional analysis and in the precepts of the Psychiatric Reform. It presents as a result the elaboration of the cartography about of the educative processes in nursing in the daily of psychiatric hospital. In accordance with the demands of the Psychiatric Reform, the role of health services should be to ensure the vision of the psychic suffering patient as a citizen, where the treatment should not be used for authority, but liberation. For this reason, it's necessary that the nursing teams participate in educative places where can reflect about the new practices of care in the psychossocial field, where can talk and hear about the implications of the Psychiatric Reform to the nursing teams and where they can remake constantly the individual and collectives relations of those teams on the psychotic individual. It was concluded that the permanent education in nursing must have as main subject the performance of the care as a social practice. It must provide that the nursing professionals seek to address the psychic suffering patients through the responsibility with the human care, with intra-team and extra-health sector arrangements, accompanying those patients in their daily, that is, in the life, respecting them in their specificities, in their peculiar choices and betting - even provisionally - to see them enjoying a social life. Thus, the educative actions in service should serve to develop new ways of understanding and interpreting the reality, to question, disagree, propose solutions, encourage the reflection in the society

Automatización en el laboratorio clínico / Automation in the clinical laboratory

La automatización de los laboratorios clínicos es un fenómeno que va en aumento, un proceso aplicable a todas las etapas del proceso analítico; el uso de la computación, la incorporación de autoanalizadores y otros elementos automáticos proporciona una serie de ventajas mejorando la eficiencia y la capacidad productiva en beneficio de los pacientes, de los profesionales y del propio laboratorio, reconocer el momento adecuado en que debe aplicarse la automatización así como el grado y etapas en las que se puede intervenir reporta una serie de beneficios, este cambio debe hacerse en forma planificada considerando elementos tales como los costos, la capacitación del personal y las tecnologías disponibles.

The automation of the clinical laboratories is a phenomenon that increases, a process applicable to all the stages of the analytical process, the use of the computation, the incorporation of autoanalyzers and other automatic elements provide a series of advantages improving the efficiency and the productive capacity to the benefit of the patients, of the professionals and the own laboratory, to recognize the suitable moment in which it must be applied to the automation as well as the degree and stages in which it is possible to be taken part reports a series of benefits, this change must be made in planned form considering elements such as the costs, the qualification of the personnel and the technologies available.

Modeling and implementation method for the automatic biochemistry analyzer control system / 中国医疗器械杂志

In this paper the system structure The automatic biochemistry analyzer is a necessary instrument for clinical diagnostics. First of is analyzed. The system problems description and the fundamental principles for dispatch are brought forward. Then this text puts emphasis on the modeling for the automatic biochemistry analyzer control system. The objects model and the communications model are put forward. Finally, the implementation method is designed. It indicates that the system based on the model has good performance.

Assessment of the performance of an automated analysis system in detecting C3 and C4 / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the performance of BNII auto-analyzer system in detecting C3 and C4.</p><p><b>METHODS</b>CLSI protocols (EP15-A, EP6-A, EP9-A2) and other relevant literatures were use to or evaluate the precision, accuracy, linearity of C3 and C4 detection by the auto-analyzer system, and the results were compared with the recognized standards.</p><p><b>RESULTS</b>The relative bias of C3 and C4 was less than one third of the CLIA'88 standard and the precision met the clinical requirement. The results tested by DADE BNII system were not compatible with those by Roche Modular System. C3 showed good linearity in the tests (R2>0.975, P<0.05) with a linearity range of 0.18-5.1 g/L. The linearity of C4 was not available because of lack of high-level samples.</p><p><b>CONCLUSION</b>The performances of DADE BNII System basically meet the recognized standards in clinical detection of C3 and C4, but the method comparison needs further validation.</p>

Application of comparison method in internal quality control of hematology analyzer by using fresh blood / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the comparison method on internal control of hematology analyzer by using fresh blood.</p><p><b>METHODS</b>The hematology analyzer with well function was selected as the reference analyzer, fresh blood samples from healthy subjects were measured by reference analyzer and the values were used to calibrate compared hematology analyzers. The acceptable limits of relative deviation of WBC,RBC, HGB,HCT, PLT were established by comparative experiments during three months. The results of fresh blood samples from patients with low/medium/high levels measured by compared analyzer were compared with those from reference analyzer, the relative deviation of WBC, RBC, HGB, HCT, PLT was calculated respectively. The internal quality control charts in laboratory information system were established, with date as x-axis, relative deviation as y-axis. The acceptable relative deviation limits were set to be +/-2 s, and to be used for laboratory quality control.</p><p><b>RESULT</b>The relative deviation of WBC, RBC, HGB, HCT, PLT with high, medium, low levels were(0.75+/-2.964)%, (1.19+/-2.488)%,(1.43+/-2.439)%; (-0.39+/-1.327)%, (-0.26+/-1.297)%, (-0.35+/-1.095)%û(-0.43+/-1.393)%, (-0.17+/-1.139)%, (0.24+/-1.166)%û(-.43+/-1.362)%, (-0.36+/-1.381)%, (-0.57+/-1.299)%û(-0.93+/-4.330)%,(0.04+/-4.118)%, (-0.41+/-4.149)%, respectively in 2006. As the second instrument, the compared analyzer was involved in College of American Pathologists Proficiency Testing with satisfactory results, the bias of WBC,RBC, HGB, HCT, PLT were within (-0.5 approximately 5.1)%, (-1.0 approximately 1.6)%, (-1.7 approximately 1.4)%, (-1.5 approximately 1.3)%, (-4.5 approximately 7.4)%, respectively.</p><p><b>CONCLUSION</b>The quality control on compared hematology analyzer can be effectively, conveniently and economically performed using this method.</p>

Evaluation of Usefulness of the Panel Test Composed of Malaria Non-specific Tests As a Surrogate Marker / 대한진단검사의학회지

BACKGROUND: Although malaria-specific antibody or antigen test is useful for the diagnosis of malaria infection, its cost-effectiveness has to be concerned in the area where malaria prevalence is very low. We created a panel test composed of malaria non-specific parameters, namely hematology autoanalyzer-derived results with or without addition of HDL-cholesterol data, and evaluated its usefulness in comparison with malaria-specific antibody test. METHODS: For 395 patients tested for malaria smear, the hematology parameters such as platelet count, NRBC (%) and VCS (volume, conductivity, scattering) parameters of WBC, and HDL-cholesterol data were analyzed. Statistical significance of each parameter and that of panel test with or without addition of HDL-cholesterol were evaluated. RESULTS: Malaria antibody test showed sensitivity of 97.1% and specificity of 99.1%. Each parameter of platelet count, NRBC (%), D parameter and HDL-cholesterol showed sensitivity of 86.8%, 41.2%, 81.8%, and 70.6%, and specificity of 85.9%, 96.3%, 72.3%, and 81.7%, respectively. Panel test without including HDL-cholesterol showed sensitivity of 91.2% and specificity of 81.6%, and that including HDL-cholesterol showed sensitivity of 91.2% and specificity of 86.2%. CONCLUSIONS: The malaria non-specific panel test composed of hematology autoanalyzer-derived parameters showed relatively good, but slightly lower sensitivity than that of malaria-specific antibody test. It might be used as a screening test for the diagnosis of malaria infection, and addition of HDL cholesterol improved little the usefulness of the panel test.

Evaluation of iQ200 Automated Urine Microscopy Analyzer / 대한진단검사의학회지

BACKGROUND: Microscopic examination of urine sediment is one of the most commonly performed tests in the clinical laboratory. However, manual microscopic sediment examination is labor-intensive, time-consuming and imprecise. In this study, we evaluated the analytical performance and clinical usefulness of a recently introduced image-based automated urinalysis system, Iris iQ200 (Iris Diagnostics, USA). METHODS: We assessed the iQ200 for linearity, precision and carryover rate using patient's samples and quality control materials. On 337 urine samples, urine sediment analyses performed by the iQ200 were compared with manual microscopy results. RESULTS: The iQ200 showed a good linearity (r2>0.99) for all cellular components analyzed. Within-run and total CVs on urine specimens and quality control samples were less than 10% except for within-run CV for the samples with low concentration of the squamous epithelial cells. The carryover rates were 0.21% for RBCs and 1.92% for WBCs. The agreement rates within one grade between the iQ200 and manual microscopy for RBCs, WBCs, and squamous epithelial cells were 93.8%, 94.2% and 96.9%, respectively. CONCLUSIONS: Since the iQ200 showed a reliable analytical performance and good concordance with manual microscopy, it could be useful in the clinical practice as a screening procedure.