RESUMEN
No abstract available.
Asunto(s)
Humanos , Basófilos/citología , Citometría de Flujo , Antígenos HLA-DR/metabolismo , Subunidad alfa del Receptor de Interleucina-3/metabolismo , Recuento de Leucocitos , Hidrolasas Diéster Fosfóricas/metabolismo , Pirofosfatasas/metabolismo , Receptores CCR3/metabolismo , Urticaria/diagnósticoRESUMEN
OBJECTIVE@#To investigate the diagnostic significance of basophil activation test (BAT) in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 (MC-CPA3), and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to β-lactam drugs and non -ionic contrast media.@*METHODS@#The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA.@*RESULTS@#The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs were significantly higher than that in normal immune group (P < 0.05).@*CONCLUSION@#There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.
Asunto(s)
Humanos , Anafilaxia/diagnóstico , Basófilos/citología , Carboxipeptidasas A/metabolismo , Complejo de Ataque a Membrana del Sistema Complemento/metabolismo , Medios de Contraste , Citometría de Flujo , Granulocitos/citología , Mastocitos/citología , Tetraspanina 30/metabolismoRESUMEN
Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of the Eighth Human Leukocyte Differentiation Workshop yielded 7 additional mAbs. Screening of mAbs generated from mice immunized with leukocytes from rabbit thymus or spleen or concanavalin A activated peripheral blood and/or spleen lymphocytes has yielded 42 mAbs that recognize species restricted epitopes expressed on one or more lineages of leukocytes. Screening of the anti-rabbit mAbs against leukocytes from other species yielded one additional mAb. The studies show that screening of existing sets of mAbs for reactivity with rabbit LDM will not be productive and that a direct approach will be needed to develop mAbs for research in rabbits. The flow cytometric approach we developed to screen for mAbs of interest offers a way for individual laboratories to identify and characterize mAbs to LDM in rabbits and other species. A web-based program we developed provides a source of information that will facilitate analysis. It contains a searchable data base on known CD molecules and a data base on mAbs, known to react with LDM in one or more species of artiodactyla, equidae, carnivora, and or lagomorpha.
Asunto(s)
Animales , Ratones , Conejos , Anticuerpos Monoclonales/inmunología , Antígenos de Diferenciación/metabolismo , Linfocitos B/citología , Basófilos/citología , Epítopos/genética , Citometría de Flujo , Regulación de la Expresión Génica , Granulocitos/citología , Leucocitos/inmunología , Monocitos/citología , Linfocitos T/citologíaRESUMEN
Inspite of the modem and sophisticated tools for the immunophenotyping of acute leukemias, yet there are still simpler methods for initial categorization of leukamias into lymphoblastic and non lymphoblastic. The aim of the present study was to identify mast cell chymase as a marker of acute myeloblastic leukemia since they originate from a common progenitor cell. Bone marrow spiration smears from leukemic patients and control were stained for chymase. The normal control had weak positive reaction, the acute lymphoblastic leukemia had negative reactions while in patients with acute myeloblastic leukemia, the chymase reaction was strikingly positive and positivity correlated well with the basophilic count in the peripheral blood and bone marrow smears. A very strong reaction was seen in patients with myeloid blast crisis and in those who were resistant to induction chemotherapy. Therefore, the chymase identification method is a very cheap and convenient tool for the cytochemical diagnosis of acute myeloblastic leukemia
Asunto(s)
Humanos , Masculino , Femenino , Quimasas/sangre , Mastocitos/metabolismo , Células de la Médula Ósea/citología , Crisis Blástica , Basófilos/citologíaAsunto(s)
Inflamación/clasificación , Inflamación/historia , Basófilos/citología , Eosinófilos/citología , Inflamación/etiología , Inflamación/patología , Inflamación/tratamiento farmacológico , Linfocitos/clasificación , Linfocitos/citología , Mastocitos/citología , Monocitos/citología , Neutrófilos/citologíaRESUMEN
In the present study 60 cases age ranging from 18-40 years were studied for variation in the absolute basophil count on the different days of menstrual cycle. At the time of ovulation a statistically significant decrease in the number of basophil count was noted. The decrease in the number of basophil at mid cycle coincided with the irregular follicle seen by sonography, which indicated ovulation. The basophil count then increased during the luteal phase. Basopenia at the time of ovulation was probably due to migration of these cells from the peripheral blood towards the rupturing follicle for the release of histamine required for ovulation.