RESUMEN
To analyze components of Citrus reticulata and salt-processed C. reticulata by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and compared the changes in components before and after being processed with salt. Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were adopted to analyze the difference in fingerprint between crude and processed C. reticulata, showing increased content of eriocitrin, limonin, nomilin and obacunone increase in salt-processed C. reticulata. Potential chemical markers were identified as limonin, obacunone and nomilin, which could be used for distinguishing index components of crude and processed C. reticulata.
Asunto(s)
Benzoxepinas , Química , Cromatografía Líquida de Alta Presión , Métodos , Citrus , Química , Análisis Discriminante , Limoninas , Química , Espectrometría de Masas , Métodos , Análisis de Componente Principal , Métodos , Sales (Química) , QuímicaRESUMEN
<p><b>OBJECTIVE</b>To develop a HPLC method to determine the content of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex simultaneously. MEHTOD: The separations were carried out at 25 degrees C on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL x min(-1), detection wavelengthes were 345 nm for jatrorrhizine , palmatine, berberine and 210 nm for obacunone.</p><p><b>RESULT</b>The average recoveries of jatrorrhizine, palmatine, berberine, and obacunone were 98.94%, 101.17%, 96.22% and 98.90%, respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate and repeatable, and can be used in content determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex.</p>
Asunto(s)
Benzoxepinas , Berberina , Alcaloides de Berberina , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos , Limoninas , Phellodendron , QuímicaRESUMEN
Através de um estudo multicêntrico duplo-cego cruzado, foram avaliados os efeitos da alpiroprida contra os de um produto de referência(oxetorona), no tratamento de enxaqueca. Os resultados obtidos com a alpiroprida foram superiores, tanto na eficácia quanto na tolerância.