RESUMEN
Proton magnetic resonance Spectroscopy (PMRS) has been used to study the differences between immortalized fibroblasts and fibrosarcoma cells of different grade. One and two dimensional purged correlation spectroscopy (PCOSY) have been used to assess intact viable fibroblast and fibrosarcoma cells, and differences in the triglyceride, cellular metabolite, and cell surface fucosylation patterns between the three cell lines have been observed. The clinical implication of this study is the potential use of PMRS as an adjunct to conventional histopathology.
Asunto(s)
Células 3T3/citología , Aminoácidos/metabolismo , Animales , Ciclo Celular , Línea Celular Transformada , Membrana Celular/metabolismo , Supervivencia Celular , Fibrosarcoma/metabolismo , Fucosa/análisis , Espectroscopía de Resonancia Magnética/métodos , Ratones , Fosfolípidos/metabolismo , Triglicéridos/metabolismo , Células Tumorales CultivadasRESUMEN
A comparison of the mechanism of action of benzoyl peroxide, a tumor promoter was studied in three different cell lines i.e. NIH 3T3, HDCS and A431. Benzoyl peroxide was found to mediate its effect by inducing poly ADP-ribosylation in all the three cell types studied but to different extents, with histone H1 serving as a common acceptor for poly ADP-ribose. It also stimulated the activities of the antioxidant enzymes CuZn superoxide dismutase and catalase in NIH 3T3 and HDCS cells, but not in A431. Alterations in the expression of c-jun and c-fos were observed in NIH 3T3 and A431 cells. Benzoyl Peroxide appeared to mediate its effect via genetic and epigenetic mechanisms.