RESUMEN
Objetivo. Analizar la percepción que el prestador de servicios de salud y el adulto mayor (AM) tienen sobre el maltrato al AM en los servicios públicos de salud, en ciudades seleccionadas de México. Material y métodos. De 2009 a 2012 se realizó un estudio con diseño cualitativo y estrategia de triangulación de fuentes de datos; se efectuaron entrevistas semiestructuradas a 13 prestadores y a 12 ancianos para recuperar su experiencia en el tema. El análisis utilizó procedimientos de la Teoría Fundamentada. Resultados. El maltrato contra el AM es una práctica naturalizada por el personal y por el anciano, la cual se manifiesta de formas diversas. Conclusiones. La institucionalización, profesionalización histórica y falta de conciencia sobre las necesidades de los AM demandan cambios de planeación, organización y supervisión del Sistema de Salud. El personal requiere intervenciones de formación, capacitación y cambio de actitudes/comportamiento, para otorgar atención integral, digna, humana y de respeto a los Derechos Humanos de los AM.
Objective. To analyze the health care providers (HCP) and elderly patients' perceptions about abuse of the elderly by health personnel of public health services, in selected cities in Mexico. Materials and methods. A qualitative study and a strategy of data triangulation were performed during 2009 and 2012; 13 HCPs and 12 elders were interviewed, in order to obtain their experience regarding elder abuse. Grounded Theory proceedings were used for the analysis. Results. Elder abuse is a naturalized practice, from HCP and elderly people's point of view; these perceptions are showed in different ways. Conclusion. Institutionalization, historical professionalization and lack of consciousness about needs of the elderly (sociocultural and economic), require changes in planning, organization and monitoring process in the Health System; training and educational interventions on staff and exchange attitudes and behavior are necessary in order to offer a health care that is comprehensive, decent, human and with respect for the human rights.
Asunto(s)
Animales , Femenino , Humanos , Ratones , Antimetabolitos Antineoplásicos/farmacología , Ciclinas/metabolismo , Inhibidores Enzimáticos/metabolismo , Fenilacetatos/farmacología , Elementos sin Sentido (Genética) , Neoplasias de la Mama , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , División Celular/efectos de los fármacos , Quinasas Ciclina-Dependientes/genética , Quinasas Ciclina-Dependientes/metabolismo , Ciclinas/genética , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Fibroblastos/enzimología , Regulación Neoplásica de la Expresión Génica/fisiología , Ratones Noqueados , Fosforilación , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , ARN Mensajero/metabolismo , Proteína de Retinoblastoma/metabolismo , Transducción de Señal/fisiología , Células Tumorales Cultivadas/citología , Células Tumorales Cultivadas/efectos de los fármacos , Células Tumorales Cultivadas/enzimología , Regulación hacia Arriba/efectos de los fármacosRESUMEN
Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptor.
Asunto(s)
Animales , Humanos , Ratones , Anticuerpos Monoclonales/química , Hemólisis/efectos de los fármacos , Inmunotoxinas/química , Receptores ErbB/metabolismo , Anémonas de Mar/química , Células Tumorales Cultivadas/efectos de los fármacos , Anticuerpos Monoclonales/inmunología , Anticuerpos Monoclonales/metabolismo , Electroforesis en Gel de Poliacrilamida , Inmunohistoquímica , Inmunotoxinas/farmacología , Receptores ErbB/efectos de los fármacos , Células Tumorales Cultivadas/citologíaRESUMEN
Retinoic acids (RA) play a key role in myeloid differentiation through their agonistic nuclear receptors (RAR alpha/RXR) to modulate the expression of target genes. In acute promyelocytic leukemia (APL) cells with rearrangement of retinoic acid receptor a (RAR alpha) (including: PML-RAR alpha, PLZF-RAR alpha, NPM-RAR alpha, NuMA- RAR alpha or STAT5b-RAR alpha) as a result of chromosomal translocations, the RA signal pathway is disrupted and myeloid differentiation is arrested at the promyelocytic stage. Pharmacologic dosage of all-trans retinoic acid (ATRA) directly modulates PML-RAR alpha and its interaction with the nuclear receptor co-repressor complex, which restores the wild-type RAR alpha/RXR regulatory pathway and induces the transcriptional expression of downstream genes. Analysing gene expression profiles in APL cells before and after ATRA treatment represents a useful approach to identify genes whose functions are involved in this new cancer treatment. A chronologically well coordinated modulation of ATRA-regulated genes has thus been revealed which seems to constitute a balanced functional network underlying decreased cellular proliferation, initiation and progression of maturation, and maintenance of cell survival before terminal differentiation.
Asunto(s)
Antineoplásicos/farmacología , Diferenciación Celular/efectos de los fármacos , Regulación Leucémica de la Expresión Génica/efectos de los fármacos , Células HL-60/citología , Humanos , Leucemia Promielocítica Aguda/tratamiento farmacológico , Proteínas de Neoplasias/efectos de los fármacos , Proteínas Nucleares/fisiología , Proteínas de Fusión Oncogénica/efectos de los fármacos , Receptores de Ácido Retinoico/antagonistas & inhibidores , Proteínas Represoras/fisiología , Receptores X Retinoide , Transducción de Señal/efectos de los fármacos , Factores de Transcripción/fisiología , Transcripción Genética/efectos de los fármacos , Translocación Genética , Tretinoina/farmacología , Células Tumorales Cultivadas/citologíaRESUMEN
O carcinoma adenóide cístico é uma neoplasia maligna que ocorre em glândulas salivares. A matriz extracelular tem sido apontada como possível fator regulador do fenótipo final desse tumor. O objetivo deste trabalho é verificar se há modificaçäo na taxa de crescimento ou induçäo de alteraçöes fenotípicas em células cultivadas de carcinoma adenóide cístico humano (células CAC2) na presença de proteínas da matriz extracelular (colágeno I, colágeno IV e laminina). A análise das curvas de crescimento realizadas mostrou que houve maior proliferaçäo celular nas culturas crescidas sobre o colágeno I. Näo houve diferenças estatísticas entre o crescimento celular de culturas tratadas com colágeno IV e laminina e o de seus respectivos controles. Nossos resultados sugerem que o colágeno I é um fator de crescimento para a linhagem celular derivada de carcinoma adenóide cístico humano
Asunto(s)
Humanos , Carcinoma Adenoide Quístico/patología , Matriz Extracelular , Neoplasias de las Glándulas Salivales/patología , División Celular , Colágeno , Laminina , Células Tumorales Cultivadas/citologíaRESUMEN
Os autores relatam o cultivo de células cutâneas, citando as células que já foram isoladas em cultura, bem como a aplicabilidade da técnica
Asunto(s)
Humanos , Masculino , Femenino , Línea Celular , Técnicas de Cultivo , Dermatología , Células Neoplásicas Circulantes , Piel/citología , Células Tumorales Cultivadas/citología , Fragmentos de Inmunoglobulinas/aislamiento & purificaciónRESUMEN
Neonatal BALB/c mice were inoculated (ip) with a recombinant Moloney murine leukemia virus-TB. Majority of the inoculated mice developed lymphoma within 5-7 months post infection. The cells from splenic lymphomas were cultured and 3 continuous cell lines (GP1, GP2 and GP3) developed. GP1 was single cell cloned and characterized. Based on Thy 1.2 (98.4%) phenotypic marker, the cell line was categorized as T cell line. The percent positivity for different cell surface markers on analysis with FACS was 98.4, 4.8, 5.5, 2.2, 1.8, 1.2 and 9.5 for Thy 1.2, mu, L3T4, Lyt2, Ia, IL2R and PNA receptor, respectively. A total of 16.5% GP1 cells was also positive for Moloney murine leukemia virus envelope protein (gp 70). Incomplete retrovirus like particles were demonstrated in the cytoplasm of GP1 cells by electron microscopy. The cell line on inoculation(ip) in neonatal BALB/c mice produced lymphomic lesions in almost all the vital organs of the mice.