RESUMEN
A combined histochemical, biochemical and electrophoretic study with respect to the enzymes succnic dehydrogenase(SDH), myofibrillar adenosine triphosphatase (m-ATPase), lactate dehydrogenase (LDH) isozymes and myosin light chains was carried out to investigate the response of rat gastrocnemius muscle (medial head). Twelve weeks after thyroidectomy, the results indicated a shift from fast to slow type pattern of LDH isozymes, fibre type transformation from Type II to Type I and a decrease in SDH and m-ATPase activity. The results suggest, possible thyroidal involvement in determining the phenotypic properties of skeletal muscle.
Asunto(s)
Adenosina Trifosfatasas/análisis , Animales , Hipotiroidismo/enzimología , Isoenzimas/análisis , L-Lactato Deshidrogenasa/análisis , Masculino , Fibras Musculares de Contracción Rápida/enzimología , Fibras Musculares de Contracción Lenta/enzimología , Proteínas Musculares/análisis , Cadenas Ligeras de Miosina/análisis , Ratas , Succinato Deshidrogenasa/análisis , Tiroidectomía/efectos adversosRESUMEN
Myosin light chains in normal and minced autografted chick gastrocnemii have been studied by disc gel electrophoresis during 70 days postgrafting period. As a result of induced mechanical trauma, the quantitative relationships between myosin light chain fractions are disturbed affecting the muscle functions.