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1.
Chinese Journal of Biotechnology ; (12): 280-291, 2024.
Artículo en Chino | WPRIM | ID: wpr-1008095

RESUMEN

In this study, the chloroplast genome of Camellia insularis Orel & Curry was sequenced using high-throughput sequencing technology. The results showed that the chloroplast genome of C. insularis was 156 882 bp in length with a typical tetrad structure, encoding 132 genes, including 88 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. Codon preference analysis revealed that the highest number of codons coded for leucine, with a high A/U preference in the third codon position. Additionally, 67 simple sequence repeats (SSR) loci were identified, with a preference for A and T bases. The inverted repeat (IR) boundary regions of the chloroplast genome of C. insularis were relatively conserved, except for a few variable regions. Phylogenetic analysis indicated that C. insularis was most closely related to C. fascicularis. Yellow camellia is a valuable material for genetic engineering breeding. This study provides fundamental genetic information on chloroplast engineering and offers valuable resources for conducting in-depth research on the evolution, species identification, and genomic breeding of yellow Camellia.


Asunto(s)
Genoma del Cloroplasto/genética , Filogenia , Fitomejoramiento , Camellia/genética , Cloroplastos/genética
2.
Zhongguo Zhong Yao Za Zhi ; (24): 5362-5371, 2021.
Artículo en Chino | WPRIM | ID: wpr-921683

RESUMEN

The present study explored the effects and its underlying mechanisms of four active fractions of Camellia nitidissima(leaf polyphenols, leaf saponins, flower polyphenols, and flower saponins in C. nitidissima) in inhibiting the proliferation and migration of non-small cell lung cancer(NSCLC) by suppressing the epidermal growth factor receptor(EGFR). MTT assay was used to detect the effect of four active fractions on the proliferation of NCI-H1975 and HCC827 cells. Wound healing assay and Transwell assay were adopted to evaluate the effect of four active fractions on the migration of NSCLC. The effect of four active fractions on the enzyme activity of EGFR was detected. Molecular docking was carried out to explore the direct action capacity and action sites between representative components of the four active fractions and EGPR. Western blot assay was employed to investigate the effect of four active fractions on the protein expression in EGFR downstream signaling pathways. The results of the MTT assay indicated that the cell viability of NCI-H1975 and HCC827 cells was significantly inhibited by four active fractions at 50, 100, 150, and 200 μg·mL~(-1) in a dose-dependent manner. Wound healing assay and Transwell assay revealed that the migration of NCI-H1975 and HCC827 cells was significantly suppressed by four active fractions. In addition, the results of the protein activity assay showed that the enzyme activity of EGFR was significantly inhibited by four active fractions. The molecular docking results confirmed that various components in four active fractions possessed strong binding activity to EGFR enzymes. Western blot assay revealed that four active fractions down-regulated the protein expression of EGFR and its downstream signaling pathways. It is concluded that the four active fractions of C. nitidissima can inhibit NSCLC. The mechanism may be related to EGFR and its downstream signaling pathways. This study provides a new scientific basis for the clinical treatment of NSCLC with active fractions of C. nitidissima, which is of reference significance for further research on the anti-tumor mechanism of C. nitidissima.


Asunto(s)
Humanos , Apoptosis , Camellia , Carcinoma de Pulmón de Células no Pequeñas/tratamiento farmacológico , Línea Celular Tumoral , Proliferación Celular , Receptores ErbB/genética , Neoplasias Pulmonares/tratamiento farmacológico , Simulación del Acoplamiento Molecular
3.
Odovtos (En línea) ; 22(1): 71-79, ene.-abr. 2020. tab, graf
Artículo en Inglés | LILACS, BBO | ID: biblio-1091507

RESUMEN

ABSTRACT The aim of this study was to explore the remineralizing effect of toothpastes based on Xilitol, Camellia Sinensis and Juniperus Communis. An in vitro experimental study was carried out in an 18 human premolars sample, which were treated with one of the 3 evaluated toothpastes and a control fluoride one. The atomic percentages of Ca and P were evaluated by Energy Dispersive X-ray Spectroscopy (EDS). In addition, the enamel surface of treated teeth was visualized by Scanning Electron Microscopy (SEM). The evaluations were carried out in three stages: pre-treatment, after an artificial demineralization process and after the treatment with the toothpastes. In the statistical analysis, the one-way ANOVA and Pearson correlation tests were used. Xilitol and Juniperus Communis showed the greatest gain of P ions compared to the control group (p<0.01). As for Ca, the group treated with xylitol-based toothpaste showed more ion gain compared with the control group (p<0.01). In the Pearson correlation test between Ca and P, statistically significant correlations were observed in all groups (p<0.01), ranging between r=0.7413 (Xylitol Group) and r=0.9510 (Control Group). We concluded that Xylitol paste showed the highest remineralizing property, both in the EDS analysis and in the SEM images.


RESUMEN El objetivo de este studio fue explorer el efecto remineralizante de las cremas dentales compuestas de Xilitol, Camelia Sinensis y Juniperus Communis. Se llevó a cabo un estudio experimental in vitro en una muestra de 18 premolares humanos, los cuales fueron tratados con una de las tres pastas dentales evaluadas y una fluorada. Los porcentajes atomicos de Ca y P fueron evaluados por Espectroscopia de rayos X de energía dispersive (EDS). Además, la superficie del esmalte de los dientes tratados fue examinada por Microscopia Electronica de Barrido (SEM). Las evaluaciones fueron llevadas a cabo en tres etapas: pre-tratamiento, despues de un proceso artificial de desmineralizacion y después del tratamiento con las pastas dentales. Se usaron ANOVA de una via y correlacion de Pearson para el analisis estadistico. Xilitol y Juniperus Communis mostraron la mayor ganancia de iones P comparados con el grupo control (p<0.01), con rangos entre r=0.7413 (Grupo con Xylitol) and r=0.9510 (Group Control). Se concluyó que la pasta con Xilitol mostró las mayores propiedades remineralizantes, tanto en el analisis EDS y las imágenes SEM.


Asunto(s)
Remineralización Dental/métodos , Pastas de Dientes , Xilitol/uso terapéutico , Técnicas In Vitro , Juniperus , Camellia
4.
Artículo en Inglés | WPRIM | ID: wpr-1010545

RESUMEN

Camellia oil has become an important plant oil in China in recent years, but its effects on non-alcoholic fatty liver disease (NAFLD) have not been documented. In this study, the effects of camellia oil, soybean oil, and olive oil on NAFLD were evaluated by analyzing the fatty acid profiles of the plant oils, the serum lipids and lipoproteins of rats fed different oils, and by cytological and ultrastructural observation of the rats' hepatocytes. Analysis of fatty acid profiles showed that the polyunsaturated fatty acid (PUFA) n-6/n-3 ratio was 33.33 in camellia oil, 12.50 in olive oil, and 7.69 in soybean oil. Analyses of serum lipids and lipoproteins of rats showed that the levels of total cholesterol and low-density lipoprotein cholesterol in a camellia oil-fed group (COFG) were lower than those in an olive oil-fed group (OOFG) and higher than those in a soybean oil-fed group (SOFG). However, only the difference in total cholesterol between the COFG and SOFG was statistically significant. Cytological observation showed that the degree of lipid droplet (LD) accumulation in the hepatocytes in the COFG was lower than that in the OOFG, but higher than that in the SOFG. Ultrastructural analysis revealed that the size and number of the LDs in the hepatocytes of rats fed each of the three types of oil were related to the degree of damage to organelles, including the positions of nuclei and the integrity of mitochondria and endoplasmic reticulum. The results revealed that the effect of camellia oil on NAFLD in rats was greater than that of soybean oil, but less than that of olive oil. Although the overall trend was that among the three oil diets, those with a lower n-6/n-3 ratio were associated with a lower risk of NAFLD, and the effect of camellia oil on NAFLD was not entirely related to the n-6/n-3 ratio and may have involved other factors. This provides new insights into the effect of oil diets on NAFLD.


Asunto(s)
Animales , Masculino , Ratas , Camellia/química , Ácidos Grasos/análisis , Hepatocitos/ultraestructura , Gotas Lipídicas/fisiología , Lípidos/sangre , Enfermedad del Hígado Graso no Alcohólico/patología , Aceites de Plantas/administración & dosificación , Ratas Sprague-Dawley
5.
Artículo en Portugués | LILACS, CONASS, ColecionaSUS, SES-GO | ID: biblio-1417336

RESUMEN

A obesidade é considerada um dos maiores problemas de saúde pública no mundo. É um fator que predispõe outras enfermidades e está relacionada ao aumento da taxa de morbidade e mortalidade. Para o tratamento da obesidade são empregados vários métodos, dentre eles o uso da fitoterapia. O presente estudo teve como objetivo avaliar as evidências científicas sobre a utilização do Phaseolus vulgaris, Cynara scolymus e Camelia sinensis no tratamento da obesidade. Trata-se de uma revisão narrativa da literatura, desenvolvida por meio de levantamento bibliográfico de artigos científicos que relacionavam os fitoterápicos com a obesidade, e publicados nos últimos 10 anos. Foi possível observar que estes fitoterápicos podem ser alternativas para tratamento da obesidade. O Phaseolus vulgaris parece proporcionar importante redução de peso devido à redução da absorção dos carboidratos. A Cynara scolymus possui frutooligossacarideos e a inulina, que alteram o trânsito intestinal e ocasionam uma rápida eliminação do bolo fecal, e, consequentemente, reduz a absorção dos lipídeos que, em excesso, contribuem para a obesidade. A Camelia sinensis aumenta a termogênese e a oxidação lipídica, o que promove gasto energético e o emagrecimento. O tratamento da obesidade pode abranger diferentes alternativas terapêuticas, ressaltando sempre a importância da utilização de fitoterápicos indicados apenas por profissionais habilitados, a fim de se alcançar resultados desejáveis e evitar efeitos adversos


Obesity is considered one of the biggest public health problems in the world. It is a factor that predisposes other diseases and it is related to the increase of the rate of morbidity and mortality. Several methods are employed to treat obesity, among them the use of herbal medicine. The present study aimed to evaluate the scientific evidence on the use of Phaseolus vulgaris, Cynara scolymus and Camelia sinensis in the treatment of obesity. This is a narrative review of the literature, developed through a bibliographical survey of scientific articles that related phytotherapeutics to obesity, and published in the last 10 years. It was possible to observe that these phytotherapics may be alternatives for the treatment of obesity. Phaseolus vulgaris appears to provide significant weight reduction due to reduced absorption of carbohydrates. Cynara scolymus has fructooligosaccharides and inulin, which alter intestinal transit and cause a rapid elimination of faecal cake, and consequently reduces the absorption of lipids that in excess contribute to obesity. Camelia sinensis increases thermogenesis and lipid oxidation, which promotes energy expenditure and weight loss. The treatment of obesity can cover different therapeutic alternatives, always emphasizing the importance of the use of phytotherapics indicated only by qualified professionals, in order to achieve desirable results and avoid adverse effects


Asunto(s)
Humanos , Phaseolus/efectos adversos , Camellia/efectos adversos , Cynara scolymus/efectos adversos , Fitoterapia , Obesidad/terapia , Pérdida de Peso , Prescripciones
6.
Arq. neuropsiquiatr ; Arq. neuropsiquiatr;77(1): 39-46, Jan. 2019. tab, graf
Artículo en Inglés | LILACS | ID: biblio-983876

RESUMEN

ABSTRACT Objective: We investigated the protective effect of the extract of the Camellia japonica L. flower on cerebral ischemia-reperfusion injury in rats. Methods: The rat ischemia-reperfusion injury was induced by middle cerebral artery occlusion for 90 minutes and reperfusion for 48 hours. The animals received an intravenous injection once a day of 20, 40, 80 mg/kg extract of C. japonica for three consecutive days before the ischemia reperfusion. The learning and memory function, the infarct volume, serum malondialdehyde (MDA) level and lactate dehydrogenase activity, and extravasation of immunoglobulin G (IgG) into cerebral parenchyma were assessed as the cell damage index. Results: Pretreatment with extract of C. japonica markedly reduced the infarct volume, serum malondialdehyde level and lactate dehydrogenase activity, and markedly inhibited the extravasation of IgG. Moreover, pretreatment with extract of C. japonica may also inhibit the learning and memory deficits induced by an ischemia-reperfusion injury. Conclusion: It was concluded that pretreatment with extract of C. japonica has a protective effect on cerebral ischemia-reperfusion injury in rats.


RESUMO Objetivo: Investigamos o efeito protetor do extrato da flor de Camellia japonica L. (ECJ) na lesão de reperfusão isquêmica cerebral (I/R) em ratos. Métodos: A lesão de I/R de rato foi induzida por uma oclusão da artéria cerebral média por 90 minutos e reperfusão por 48 horas. Os animais receberam uma injeção intravenosa uma vez ao dia de 20, 40, 80 mg/kg de ECJ por três dias consecutivos antes da I/R. A função de aprendizagem e memória, o volume do infarto, o nível sérico de malondialdeído (MDA), a atividade da desidrogenase láctica e o extravasamento de imunoglobulina (IgG) no parênquima cerebral foram avaliados como índices de dano celular. Resultados: O pré-tratamento com ECJ reduziu acentuadamente o volume do infarto, o nível sérico de MDA e a atividade da desidrogenase láctica, e inibiu marcadamente o extravasamento de IgG. Além disso, o pré-tratamento com ECJ também poderia inibir os déficits de aprendizado e memória induzidos pela lesão de I/R. Conclusão: O pré-tratamento com ECJ tem um efeito protetor contra lesão cerebral de I/R em ratos.


Asunto(s)
Animales , Masculino , Femenino , Extractos Vegetales/farmacología , Daño por Reperfusión/prevención & control , Isquemia Encefálica/prevención & control , Fármacos Neuroprotectores/farmacología , Camellia/química , Natación/fisiología , Factores de Tiempo , Inmunoglobulina G/sangre , Nimodipina/farmacología , Distribución Aleatoria , Reproducibilidad de los Resultados , Resultado del Tratamiento , Ratas Sprague-Dawley , Modelos Animales de Enfermedad , L-Lactato Deshidrogenasa/análisis , Malondialdehído/sangre
7.
Artículo en Coreano | WPRIM | ID: wpr-719519

RESUMEN

Exogenous lipoid pneumonia is an uncommon medical condition resulting from aspiration or inhalation of oily material. Generally, lipoid pneumonia has nonspecific clinical and radiological presentations, and may be misdiagnosed as bacterial pneumonia or lung cancer. We describe an unusual case of exogenous lipoid pneumonia accompanied by peripheral blood and pulmonary eosinophilia. A 63-year-old man was admitted with progressively worsening exertional dyspnea and productive cough for 5 days. A chest radiograph showed abnormalities in the lower lobe of the right lung, and a diagnosis of community-acquired pneumonia was made; intravenous antibiotics were administered. However, dyspnea and hypoxia gradually worsened and peripheral blood eosinophilia developed. A bronchoscopy was performed and bronchoalveolar lavage fluid analysis showed markedly increased numbers of eosinophils (40%). Subsequently, a comprehensive review of history revealed that he fell asleep with camellia oil in his mouth for 2 weeks to relieve foreign body sensation of the throat. Sputum and bronchoalveolar lavage fluid cytology showed the presence of lipid-laden macrophages. He was diagnosed with lipoid pneumonia and acute eosinophilic pneumonia. Chest radiograph and symptom were rapidly improved after treatment with intravenous methylprednisolone.


Asunto(s)
Humanos , Persona de Mediana Edad , Hipoxia , Antibacterianos , Líquido del Lavado Bronquioalveolar , Broncoscopía , Camellia , Tos , Diagnóstico , Disnea , Eosinofilia , Eosinófilos , Cuerpos Extraños , Inhalación , Pulmón , Neoplasias Pulmonares , Macrófagos , Metilprednisolona , Boca , Faringe , Neumonía , Neumonía Bacteriana , Neumonía Lipoidea , Eosinofilia Pulmonar , Radiografía Torácica , Aspiración Respiratoria , Sensación , Esputo
8.
Artículo en Inglés | WPRIM | ID: wpr-741689

RESUMEN

BACKGROUND/OBJECTIVES: Sageretia thea is traditionally used as a medicinal herb to treat various diseases, including skin disorders, in China and Korea. This study evaluated the inhibitory effect of Sageretia thea fruit on melanogenesis and its underlying mechanisms in B16F10 mouse melanoma cells. The active chemical compounds in anti-melanogenesis were determined in Sageretia thea. MATERIALS/METHODS: Solvent fractions from the crude extract were investigated for anti-melanogenic activities. These activities and the mechanism of anti-melanogenesis in B16F10 cells were examined by determining melanin content and tyrosinase activity, and by performing western blotting. RESULTS: The n-hexane fraction of Sageretia thea fruit (HFSF) exhibited significant anti-melanogenic activity among the various solvent fractions without reducing viability of B16F10 cells. The HFSF suppressed the expression of tyrosinase and tyrosinase-related protein 1 (TRP1). The reduction of microphthalmia-associated transcription factor (MITF) expression by the HFSF was mediated by the Akt/glycogen synthase kinase 3 beta (GSK3β) signaling pathway, which promotes the reduction of β-catenin. Treatment with the GSK3β inhibitor 6-bromoindirubin-3'-oxime (BIO) restored HFSF-induced inhibition of MITF expression. The HFSF bioactive constituents responsible for anti-melanogenic activity were identified by bioassay-guided fractionation and gas chromatography-mass spectrometry analysis as methyl linoleate and methyl linolenate. CONCLUSIONS: These results indicate that HFSF and its constituents, methyl linoleate and methyl linolenate, could be used as whitening agents in cosmetics and have potential for treating hyperpigmentation disorders in the clinic.


Asunto(s)
Animales , Ratones , Ácido alfa-Linolénico , Blanqueadores , Western Blotting , Camellia , China , Frutas , Cromatografía de Gases y Espectrometría de Masas , Hiperpigmentación , Corea (Geográfico) , Ácido Linoleico , Melaninas , Melanoma , Factor de Transcripción Asociado a Microftalmía , Monofenol Monooxigenasa , Fosfotransferasas , Plantas Medicinales , Piel
9.
Mycobiology ; : 85-91, 2018.
Artículo en Inglés | WPRIM | ID: wpr-729997

RESUMEN

Endophytic fungi strains (n = 81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson’s and Shannon’s diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.


Asunto(s)
Agentes de Control Biológico , Camellia , Colon , Endófitos , Frutas , Hongos , Fusarium , Penicillium
10.
Artículo en Inglés | WPRIM | ID: wpr-209969

RESUMEN

The chemical components and biological activity of Camellia mistletoe, Korthalsella japonica (Loranthaceae) are relatively unknown compared to other mistletoe species. Therefore, we investigated the phytochemical properties and biological activity of this parasitic plant to provide essential preliminary scientific evidence to support and encourage its further pharmaceutical research and development. The major plant components were chromatographically isolated using high-performance liquid chromatography and their structures were elucidated using tandem mass spectrometry and nuclear magnetic resonance anlysis. Furthermore, the anti-inflammatory activity of the 70% ethanol extract of K. japonica (KJ) and its isolated components was evaluated using a nitric oxide (NO) assay and western blot analysis for inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. Three flavone di-C-glycosides, lucenin-2, vicenin-2, and stellarin-2 were identified as major components of KJ, for the first time. KJ significantly inhibited NO production and reduced iNOS and COX-2 expression in lipopolysaccharide-stimulated RAW 264.7 cells at 100 μg/mL while similar activity were observed with isolated flavone C-glycosides. In conclusion, KJ has a simple secondary metabolite profiles including flavone di-C-glycosides as major components and has a strong potential for further research and development as a source of therapeutic anti-inflammatory agents.


Asunto(s)
Antiinflamatorios , Western Blotting , Camellia , Cromatografía Liquida , Etanol , Espectroscopía de Resonancia Magnética , Muérdago , Óxido Nítrico , Óxido Nítrico Sintasa , Estructuras de las Plantas , Plantas , Prostaglandina-Endoperóxido Sintasas , Espectrometría de Masas en Tándem , Viscaceae
11.
Natural Product Sciences ; : 270-274, 2016.
Artículo en Inglés | WPRIM | ID: wpr-146017

RESUMEN

Green tea, the leaves of Camellia sinsneis (Theaceae), is generally acknowledged as the most consumed beverage with multiple pharmacological functions including antioxidant activity. This study was performed to analyze the effect of extraction conditions of green tea on its antioxidant effects using DPPH assay. Three extraction factors such as extraction solvent (EtOH, 0 – 100%), extraction time (3 – 15 min) and extraction temperature (10 – 70℃) were analyzed and optimized extraction condition for antioxidant activity of green tea extract (GTE) was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of data and the optimal conditions of extraction were found to be 57.7% EtOH, 15 min and 70℃. Under this condition, antioxidant activity of experimental data was 88.4% which was almost fit to the ideal value of 88.6%. As epigallocatechin gallate (EGCG) is known for the major ingredient for antioxidant activity of green tea, we investigated the effect of EGCG on antioxidant activity of GTE. EGCG showed antioxidant activity with the IC50 value of 4.2 µg/ml and a positive correlation was observed between EGCG content and the antioxidant activity of GTE with R2 = 0.7134. Interestingly, however, GTE with 50 – 70% antioxidant activity contain less than 1.0 µg/ml of EGCG, which is much lower than IC50 value of EGCG. Therefore, we suppose that EGCG together with other constituents contribute to antioxidant activity of GTE. Taken together, these results suggest that green tea is more beneficial than EGCG alone for antioxidant ability and optimal extraction condition of green tea will be useful for the development of food and pharmaceutical applications.


Asunto(s)
Antioxidantes , Bebidas , Camellia , Concentración 50 Inhibidora ,
12.
Artículo en Coreano | WPRIM | ID: wpr-125571

RESUMEN

Although the effects of the rice bran have recently been investigated, there is no information regarding platelet physiology available. However, it is well known that fermented natural plants have a beneficial effect on cardiovascular diseases. Therefore, this study was conducted to investigate whether fermented rice bran extract (FRBE) with several plants (Artemisia princeps, Angelica Gigantis Radix, Cnidium officinale, and Camellia sinensis) affected agonist-induced platelet aggregation, and if so, what the underlying mechanism of its activity was. We performed several experiments, including in vitro platelet aggregation, intracellular calcium concentration and adenosine triphosphate release. In addition, the activation of integrin alphaIIbbeta3 was determined using fibrinogen binding. Thrombus formation was also evaluated in vivo using an arterio-venous shunt model. The FRBE inhibited collagen-induced platelet aggregation in a concentration-dependent manner. FRBE significantly and dose dependently attenuated thrombus formation using rat arterio-venous shunt. FRBE suppressed the intracellular calcium mobilization in collagen-stimulated platelets. We also found that FRBE inhibited extracellular stimuli-responsive kinase 1/2, p38-mitogen-activated protein kinases and c-Jun N-terminal kinase phosphorylation. These results suggested that FRBE inhibited collagen-induced platelet aggregation, which was mediated by modulation of downstream signaling molecules. In conclusion, FRBE could be developed as a functional food against aberrant platelet activation-related cardiovascular diseases.


Asunto(s)
Animales , Ratas , Adenosina Trifosfato , Angelica , Plaquetas , Calcio , Camellia , Enfermedades Cardiovasculares , Cnidium , Colágeno , Fibrinógeno , Alimentos Funcionales , Proteínas Quinasas JNK Activadas por Mitógenos , Fosforilación , Fosfotransferasas , Fisiología , Agregación Plaquetaria , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria , Proteínas Quinasas , Trombosis
13.
Indian J Exp Biol ; 2013 Jun; 51(6): 458-463
Artículo en Inglés | IMSEAR | ID: sea-147614

RESUMEN

Camellia oleifera Abel. [C. oleosa (Lour.) Rehd.], an evergreen plant, is used for healthful oil production, but the shells are always discarded and need to be utilized. The present study was undertaken to explore the effect of extracts from the shells of C. oleifera on adjusting cardiovascular system. A flavonoid was obtained by reflux extraction of the shells in 70% methanol, hydrolysis in 2 M hydrochloric acid, and crystallization in acetone. Its structure was identified as a novel biflavonoid. Mice model of hyperlipidemia was setup by high fat diet for 30 d to evaluate the hypolipidemic effect of the biflavonoid at dose of 50, 100 and 200 mg/kg/d (ig). Antioxidative activity was determined by levels of malondialdehyde (MDA), superoxidase dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice serum. The biflavonoid significantly controlled mice weight and liver coefficient, decreased the content of total cholesterol and triglyceride, promoted the level of high density lipoprotein in a dose dependent manner. The significant decrease of MDA content and increase of SOD and GSH-Px activity indicated it enhanced antioxidative capacity in vivo and was ascribed to hypolipidemic effect. The biflavonoid is useful in the prevention of high fat diet induced hyperlipidemia.


Asunto(s)
Animales , Antioxidantes/farmacología , Biflavonoides/química , Biflavonoides/aislamiento & purificación , Biflavonoides/farmacología , Peso Corporal/efectos de los fármacos , Camellia/química , Glutatión Peroxidasa/metabolismo , Hiperlipidemias/tratamiento farmacológico , Lípidos/análisis , Masculino , Malondialdehído/metabolismo , Ratones , Estructura Molecular , Oxidación-Reducción , Fitoterapia , Superóxido Dismutasa/metabolismo
14.
Rev. colomb. gastroenterol ; 28(1): 46-52, ene.-mar. 2013. ilus, tab
Artículo en Español | LILACS | ID: lil-678056

RESUMEN

El consumo de té verde se ha visto aumentado en los últimos años y así mismo un incremento en el reportede sus efectos adversos y toxicidad hepática, que aparentemente se dan por procesos de interacción enzimáticay daño celular, interferencia con sistemas de respuesta biológica y reacciones metabólicas. Esta revisiónse introduce con las características morfológicas y componentes bioquímicos de la planta Camellia sinensis.Posteriormente, por medio del análisis de ensayos clínicos, ensayos in vitro, estudios farmacodinámicos yfarmacocinéticos, se comprenden algunos de los mecanismos mediante los cuales los componentes del téverde causan daño hepático; por ejemplo, las interacciones con enzimas como la UDPGT, alcohol deshidrogenasa,citocromo P450 y otras como la del sistema enzimático mitocondrial e inmune. Estas formas delesión celular se correlacionaron con reportes de caso en la literatura científica, permitiendo ver los espectrosde daño hepático asociados a su consumo. A partir de este análisis se encuentra que aún los mecanismosde toxicidad hepática por el té verde son inciertos; sin embargo, se han involucrado ciertas catequinas de laplanta Camellia sinensis y ciertas interacciones a nivel celular y mitocondrial que pueden ser las responsablesde su toxicidad. Por esto se pueden justificar medidas preventivas a nivel social y político sobre la ingesta de este producto naturista


As consumption of green tea has increased in recent years, so too have reports of its adverse effects. Hepatotoxicity is apparently caused by enzymatic interaction that leads to cellular damage and interference with biological response systems and metabolic reactions. This review article introduces the morphological characteristics and biochemical components of the green tea plant, camellia sinensis. Analysis of clinical trials, in-vitro trials and pharmacodynamic and pharmacokinetic studies then shed light on some of the mechanisms by which green tea causes hepatic damage. Examples are the chemical interactions with enzymes such as UDPGT, alcohol dehydrogenase and cytochrome P450 and interactions with the mitochondrial enzyme and immune systems. These forms of cellular lesions are correlated with case reports in the scientific literatura which clarify the spectrum of hepatic damage associated with the consumption of green tea. This analysis finds that even though the mechanisms by which green tea causes hepatic toxicity are still a mystery, certain catechins of camellia sinensis and interactions at the cellular and mitochondrial levels may be responsible for this toxicity. On this basis, social and political preventive measures regarding intake of this natural product at levels can be justified


Asunto(s)
Anciano , Camellia , Toxicidad
15.
Artículo en Inglés | WPRIM | ID: wpr-155359

RESUMEN

The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration (LC50) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had LC50 at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides.


Asunto(s)
Animales , Camellia/química , Vectores de Enfermedades , Garcinia mangostana/química , Gastrópodos/efectos de los fármacos , Interacciones Huésped-Parásitos , Extractos Vegetales/química , Semillas/química
16.
Artículo en Inglés | WPRIM | ID: wpr-335031

RESUMEN

<p><b>OBJECTIVE</b>To isolate Salmonella from curry samples and to evaluate the drug sensitivity of the food-borne Salmonella and its susceptibility to specific plant extracts.</p><p><b>METHODS</b>Salmonella was isolated from the curry samples by standard microbiological methods and was confirmed by biochemical tests. The antibiotic susceptibility test was conducted by disc diffusion method using commercially available antibiotics such as ampicillin, tetracycline, chloramphenicol, kanamycin, and penicillin. In addition, the susceptibility of the food-borne Salmonella was also evaluated against the aqueous extracts of Camelia sinensis (L.) Theaceae (tea leaves) and the Trachyspermum ammi (L.) Apiaceae ( ajwain or omum seeds).</p><p><b>RESULTS</b>Out of fifty curry samples, only seven samples were identified to have Salmonella contamination. The Salmonella isolates showed a significant drug resistance pattern except for kanamycin. The plant extracts showed a considerable antibacterial activity against the isolates, indicating the presence of antimicrobial principle which can be exploited after complete pharmacological investigations.</p><p><b>CONCLUSIONS</b>The present study demonstrates the occurrence of Salmonella in the curry samples, and shows significant drug resistance against most of the commercially available antibiotics, except kanamycin. Antimicrobial effect of the plant extracts against the food-bone Salmonella suggests that dietary including medicinal herbs would be one strategy to manage food borne pathogens.</p>


Asunto(s)
Humanos , Antibacterianos , Farmacología , Apiaceae , Química , Camellia , Química , Farmacorresistencia Bacteriana , Microbiología de Alimentos , Pruebas de Sensibilidad Microbiana , Extractos Vegetales , Farmacología , Salmonella
17.
Mycobiology ; : 71-78, 2011.
Artículo en Inglés | WPRIM | ID: wpr-729241

RESUMEN

The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial beta-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.


Asunto(s)
Biodiversidad , Camellia , Bases de Datos de Ácidos Nucleicos , ADN , Hongos , India , Plantas Medicinales , Potentilla , Tubulina (Proteína)
18.
Mycobiology ; : 152-154, 2009.
Artículo en Inglés | WPRIM | ID: wpr-729934

RESUMEN

Lichen genus Sarcographa Fee, a stromatoid Graphidacean taxa, was newly found in Geomun Island, Jeonnam province. The lichen grew on the bark of Camellia japonica and Eurya emarginata along the coastal line of the island. It was identified as Sarcographa tricosa (Ach.) Mull. Arg. for the first time in Korea.


Asunto(s)
Ascomicetos , Camellia , Honorarios y Precios , Corea (Geográfico) , Líquenes
19.
Artículo en Coreano | WPRIM | ID: wpr-26539

RESUMEN

Lower back pain commonly develops following spinal anesthesia. This pain is likely caused by strain on the ligaments associated with paraspinous muscle relaxation and positioning during the operation. We describe a case involving a healthy 61-year-old woman with a varicose vein that was scheduled for phlebotomy under spinal anesthesia. Two days after spinal anesthesia, the patient experienced severe lower back pain that was markedly aggravated by twisting and extension of the spine, but subsided with rest. Immediately after a lumbar medial branch block was performed at the area of tenderness the pain subsided. Thease results suggest that immediate treatment of acute lower back pain is important for preventing progression to chronic low back pain.


Asunto(s)
Femenino , Humanos , Persona de Mediana Edad , Anestesia Raquidea , Dolor de Espalda , Camellia , Ligamentos , Dolor de la Región Lumbar , Relajación Muscular , Flebotomía , Columna Vertebral , Esguinces y Distensiones , Várices
20.
Artículo en Coreano | WPRIM | ID: wpr-221633

RESUMEN

There are variable types in wrist joint injury. Most common case is simple distal radius fracture. And ulnar head dislocation associated with disruption of distal radioulnar ligament is unusual. Among thease injury types. volar dislocation of ulnar head in the distal radioulnar joint is not common and it is misdiagnosis frequently. So it needs to surgical operation frequently. The author reviews this injury with the relevant literature.


Asunto(s)
Camellia , Errores Diagnósticos , Luxaciones Articulares , Cabeza , Articulaciones , Ligamentos , Fracturas del Radio , Articulación de la Muñeca
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