Coordinate production of cephamycin c and clavulanic acid by Streptomyces clavuligerus.

Production of cephamycin c and clavulanic acid by Streptomyces clavuligerus was investigated using different media in shake flask condition. Highest cell growth (3.8 g/L) was observed in glycerol, sucrose, proline and glutamic acid (GSPG) medium. Although, GSPG medium supported maximum growth, it was least effective for the synthesis of both cephamycin and clavulanic acid. Yield of cephamycin and clavulanic acid was maximum in dextrin and K medium, respectively. High and low level of constituents of dextrin medium, affected production of both cephamycin and clavulanic acid. Biosynthesis of clavulanic acid was associated with production of cephamycin c.

Effect of support materials on cephamycin C production by immobilized Streptomyces clavuligerus.

In order to know the effect of supports on cephamycin C production, under similar experimental conditions, S. clavuligerus cells were immobilized with--sponge, 2% agar, 2% and 4% alginate support materials. An experimental set of free cell was also maintained as control. Cephamycin C production by these immobilized and free cells was estimated at 48, 96 and 120 hr of fermentation. In all the cases cephamycin C production was found to be high at 120 hr of fermentation. Sponge was found to be a better support material than other supports used for immobilization.