Detección y caracterización molecular de Chlamydia psittaci y Chlamydia abortus en psitácidos mascotas en la provincia de Buenos Aires, Argentina / Detection and molecular characterization of Chlamydia psittaci and Chlamydia abortus in psittacine pet birds in Buenos Aires province, Argentina

In order to determine the presence and genetic diversity of Chlamydia spp. in the north-eastern area of Buenos Aires province, Argentina, conjunctival, oropharyngeal, cloacal swab and tissues were collected from a total of 90 psittacine pet birds of different age and clinical manifestations. Through molecular methods, Chlamydiaceae was detected in 30% (27/90) of the samples, out of which 70.3% (19/27) were positive for Chlamydia psittaci and 14.9% (4/27) for Chlamydia abortus. Nine C. psittaci positive samples were genotyped by ompA gene sequences, 8 clustered within genotype A and 1 within genotype B. A significant association was observed between the presence of Chlamydia spp. and the manifestation of clinical signs compatible with chlamydiosis, as well as with the age of the birds (younger than one year old). This report contributes to the improvement of our understanding of chlamydial agents in our country.

Con el objetivo de determinar la presencia de Chlamydia spp. en psitácidos del área noreste de la provincia de Buenos Aires y conocer su diversidad genética, se recolectaron y analizaron mediante métodos moleculares hisopados conjuntivales, orofaríngeos, cloacales y tejidos de un total de 90 psitácidos de diferentes edades y con diversas manifestaciones clínicas. El 30% (27/90) de las muestras procesadas fueron positivas para Chlamydiaceae; el 70,3% (19/27) de estas resultaron positivas para Chlamydia psittaci y el 14,9% (4/27) para Chlamydia abortus. Nueve muestras positivas para C. psittaci fueron genotipificadas por secuenciación del gen ompA: 8 correspondieron al genotipo Ay una al genotipo B. Se observó una asociación significativa entre la presencia de Chlamydia spp. y la manifestación de signos clínicos compatibles con clamidiosis, como así también con la edad de las aves (menores de un ano). Este informe contribuye a mejorar nuestro conocimiento de los agentes clamidiales en nuestro país.

Species specific chlamydiae antibodies in voluntary blood donors of Delhi.

BACKGROUND & OBJECTIVES: Different species of genus Chlamydia have been associated with ocular, genitourinary and respiratory infections, and coronary artery disease. Since the majority of these infections remain asymptomatic or subclinical, antibodies may be present in apparently healthy individuals, and the determination of species specific Chlamydia antibodies in a healthy population may reflect exposure. We therefore screened the sera of healthy blood donors for species specific Chlamydia antibodies by microimmunofluorescence assay. METHODS: Sera of 844 voluntary blood donors from Delhi were screened by microimmunofluorescence assay using specific antigens of C. trachomatis (18 serovars divided in 3 pools of serotypes A-C, D-K and L1-L3), C. psittaci and C. pneumoniae for Chlamydia antibodies. RESULTS: A total of 470 (55.69%) blood donors were found positive for Chlamydia antibodies. Of these, 361(42.77%) were positive for C. pneumoniae, 106 (12.5%) for C. trachomatis [of which 72 (8.5%) were against serotypes D-K and 34(4%) were against serotypes A-C]. There donors (0.3%) had antibodies to C. psittaci. INTERPRETATION & CONCLUSION: The results suggested that more than half of the study population (55.69%) is exposed to one or other species of chlamydiae. Majority of the donors (44.7%) had C. pneumoniae antibodies, suggesting the presence of widespread apparent or inapparent C. pneumoniae infection. The findings also suggest that Chlamydia antibody testing for the diagnosis of chlamydial infections may not be helpful due to the presence of antibodies in a large proportion of healthy individuals.

Infección del cuello uterino por chlamydia trachomatis: incidencia y comparación de 2 agentes terapéuticos / Cervical infection with chlamydia trachomatis: incidence and comparation of 2 therapeutic agents

Se presenta un estudio prospectivo con 253 pacientes asintomáticas que acudieron a la consulta de ginecología en 1994. La incidencia de infección cervical por Chlamydia Trachomatis fue de 28,8 por ciento en nuestro grupo. Las 52 pacientes positivas al test Cherview de Chlamydia fueron divididas en dos grupos, el grupo 1 recibió tratamiento con azitromicina, 1.000 mg. Una sola dosis, las pacientes del grupo 2 recibieron roxitromicina 300 mg/día durante 7 días. El 94,2 por ciento de las pacientes en cada grupo presentó erradicación del patógeno. No hubo recaídas a los tres meses. Ninguna paciente presentó efectos colaterales

Chlamydia trachomatis na patoologia médica: revisäo / Chamydia trachomatis in the medical patologic

Clamídias säo bactérias que, no homem, provocam: tracom ocular, tracoma genital, conjuntivite de inclusäo, linfogranuloma venéreo, síndrome de Reiter e a psitacose. É a maior causa de doença sexualmente transmitida, especialmente entre adolescentes e adultos jovens. No presente trabalho säo estudados, além de outros, os aspectos morfológicos, patogênicos e epidemiológicos