RESUMEN
ABSTRACT This work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, catalase and, nitrogenase. All isolates were able to metabolize sucrose and malic acid in contrast with mannitol, which was metabolized only by one isolate. For the other carbon sources, we observed a great variability in its use by the isolates. Most isolates showed antibiosis against Rhizoctonia solani (75%) and Sclerotinia sclerotiorum (55%) and, 50% of them showed antibiosis against both pathogens. Six isolates showed simultaneous ability of antibiosis, inorganic phosphate solubilization and protease activity. Based on phylogenetic analysis of the 16S rRNA gene all the isolates belong to Bacillus genus. Under greenhouse conditions, two isolates (S4 and S22) improved to about 24%, 25%, 30% and 31% the Total N, leaf area, shoot dry weight and root dry weight, respectively, of rice plants, indicating that they should be tested for this ability under field conditions.
Asunto(s)
Bacterias/aislamiento & purificación , Chryseobacterium/genética , Oryza/crecimiento & desarrollo , Microbiología del Suelo , Antibiosis , Fenómenos Fisiológicos Bacterianos , Bacterias/clasificación , Bacterias/genética , Composición de Base , Secuencia de Bases , Chryseobacterium/clasificación , Chryseobacterium/efectos de los fármacos , Chryseobacterium/aislamiento & purificación , ADN Bacteriano/genética , Datos de Secuencia Molecular , Oryza/microbiología , FilogeniaRESUMEN
ABSTRACT The type strain SUR2 of the novel species Chryseobacterium limigenitum was isolated from a dehydrated sludge of the municipal sewage treatment plant in Dogoše near Maribor in Slovenia. The draft genome, with 60 contigs, 4,697,725 bp, 34.4% of G+C content, was obtained using the Illumina HiSeq 2500-1 platform. Joint Genome Institute Microbial Genome Annotation Pipeline (MGAP v.4) has identified 4322 protein-coding sequences including resistance genes against arsenic and other heavy metals. In addition, a subclass B3 metallo-β-lactamase, which confers resistance to penicillins, cephalosporins and carbapenems, was also present in the genome. The genome sequence provides important information regarding bioremediation potential and pathogenic properties of this newly identified species.
Asunto(s)
Aguas del Alcantarillado/microbiología , Genoma Bacteriano , Chryseobacterium/genética , Penicilinas/farmacología , Filogenia , Aguas del Alcantarillado/química , Composición de Base , ADN Bacteriano/genética , Datos de Secuencia Molecular , Secuencia de Bases , Pruebas de Sensibilidad Microbiana , Carbapenémicos/farmacología , Chryseobacterium/aislamiento & purificación , Chryseobacterium/clasificación , Chryseobacterium/efectos de los fármacos , Antibacterianos/farmacologíaRESUMEN
Chryseobacterium species is an uncommon human pathogen although recovered from various sources in the hospital environment. Most infections have been detected in hospitalized patients with severe underlying diseases and who had indwelling devices or implants. Despite their low virulence, chryseobacteria are inherently resistant to many antimicrobial agents. We report a rare case of urinary tract infection by Chryseobacterium indologenes in a young girl, operated for renal calculus and successfully treated with piperacillin-tazobactam combination.