Chicken collagen type II reduces articular cartilage destruction in a model of osteoarthritis in rats / Colágeno de pollo tipo ii reduce la destrucción del cartílago articular en un modelo de osteoartritis en ratas

AIM: To evaluate the therapeutic effects of domestic chicken collagen type II (CCII) on rat osteoarthritis (OA) and analyze concomitant changes in the level of Matrix metalloproteinase (MMP)-13, MMP-9, Cathepsin K and their mRNA as well as the tissue inhibitor of matrix metalloproteinase (TIMP)-1 mRNA in articular cartilage of osteoarthritic rats. METHODS: Osteoarthritis models were surgically induced. Morphology of articular cartilage was done by haematoxylin and eosin staining and Mankin score was calculated, immunohistochemistry of MMP-13, MMP-9 and Cathepsin K was done by ABC method while the mRNA level for MMP-13, MMP-9, cathepsin K as well as TIMP-1 was evaluated by RT-PCR method. RESULTS: Oral administration of CCII reduced the morphological changes of osteoarthritic cartilage (shown by Mankin score), decreased levels of MMP-13, MMP-9, cathepsin K as well as their mRNA in articular cartilage from osteoarthritic rats while it exhibited no effect on TIMP-1 mRNA. CONCLUSION: Oral CCII reduced articular cartilage degradation of osteoarthritic rats and may probably be a potent drug candidate for OA treatment.

Objetivo: Evaluar los efectos terapéuticos del colágeno de pollo doméstico de tipo II (CPII) en la osteoartritis de ratas (OA) y analizar los cambios concomitantes en el nivel de metaloproteinasa de la matriz (MMP-13). MMP-9, catepsina K y su mRNA, así como el inhibidor tisular de la metalopro-teinasa de la matriz (TIMP)-1 mRNA en el cartílago articular de ratas osteoartríticas. Métodos: Modelos osteoartríticos fueron obtenidos mediante inducción quirúrgica. La morfología del cartílago articular se realizó mediante tinción H-E, y se calculó la puntuación de Mankin. Se realizó la inmunohistoquímica de MMP-13, MMP-9 y catepsina K mediante el método ABC, en tanto que el nivel de mRNA para MMP-13, MMP-9, y catepsina K así como el TIMP-1, fue evaluado mediante el método RT-PCR. Resultados: La administración oral de CPII redujo los cambios morfológicos del cartílago osteo-artrítico (mostrado por la puntuación Mankin), disminuyó los niveles de MMP-13, MMP-9, catepsina K así como su mRNA en cartílago articular de las ratas osteoartríticas mientras que no mostró efecto sobre TIMP-1 mRNA. Conclusión: El CP oral redujo la degradación del cartílago articular de las ratas osteoartríticas y puede ser probablemente candidato a un medicamento potente para el tratamiento de la OA.

Cultura de condrócitos humanos para tratamento de lesões cartilaginosas / Human chondrocytes culture to treatment of cartilaginous injuries

Este trabalho apresenta como objetivo testar o cultivo de condrócitos por um método não enzimático. Para isto, foram isolados condrócitos de cartilagem costal humana pelo método de explante primário, em que as células migraram da cartilagem e se aderiram ao fundo do frasco de cultura. posteriormente, pelo método de imunofluorescência indireta dosou-se a capacidade de produção de colágeno tipo II pelos condrócitos isolados a fim de / This work presents as objective to test culture of chondrocytes for a not enzymatic method of primary explante, where the cells migrate of the cartilage and if they had adhered to the deep one in the bottle of culture. Later, for the method of indirect immunofluorescence it was dosed capacity of production of collagen type II for the isolated chondrocytes in order to evaluate the capacity of the cells to produce extracellular matrix to demonstrate the maintenance of the physiological capacities of the isolated cells in culture...