RESUMEN
28-Homobrassinolide (28-HBL), a brassinosteroid is reported to play significant role in diverse physiological processes. It induces a range of cellular and adaptive responses to a range of environmental stresses. Cadmium (Cd) is a non-essential metal which alters various physiological processes and generates ROS, which can oxidize biological macromolecules and causes oxidative stress. This stress is generally overcome by the internal antioxidative defense system and stress shielding phytohormones. In this study, effect of 28-HBL was studied on growth and activities of antioxidant enzymes in known hyperaccumulator Raphanus sativus L. (radish) seedlings grown under cadmium (Cd) metal stress. To determine the influence of 28-HBL (0, 10-11, 10-9, 10-7 M) in radish seedlings subjected to Cd (0, 0.5, 1.0, 1.5 mM) stress, the activities of antioxidant enzymes (APOX, CAT, GR, POD and SOD) were analyzed. In addition, length and biomass of radish seedlings was also recorded. Cd toxicity resulted in reduced length, biomass, protein content and activities of antioxidant enzymes. 28-HBL treatments lowered the Cd toxicity by enhancing the activities of antioxidant enzymes, biomass and seedling length. The present study thus suggests a possible role of 28-HBL in amelioration of metal stress by regulating the activities of antioxidant enzymes in radish.
Asunto(s)
Antioxidantes/metabolismo , Cadmio/toxicidad , Colestanonas/farmacología , Estrés Oxidativo/efectos de los fármacos , Reguladores del Crecimiento de las Plantas/farmacología , Raphanus/efectos de los fármacos , Raphanus/enzimología , Raphanus/crecimiento & desarrollo , Plantones/efectos de los fármacos , Plantones/enzimología , Plantones/crecimiento & desarrolloRESUMEN
<p><b>OBJECTIVE</b>To investigate the triterpenoids chemical constituents in rhizome of Alisma gramineum.</p><p><b>METHOD</b>Silicon gel chromatography and HPLC techniques were employed for isolation and purification of the constituents, and the structures were elucidated by various spectral analyses such as NMR, MS and IR.</p><p><b>RESULT</b>5 protostane-type tetracyclic triterpenoids were isolated and identified as 16,23-oxidoalisol B(1), 11-deoxyalisol C(2), alisol F(3), 16beta-methoxyalisol B acetate (4), 16beta-hydroxyalisol B acetate (5).</p><p><b>CONCLUSION</b>All of these compounds were found from A. gramineum for the first time.</p>