In-Vitro Study of Antimicrobial Activities of Five Different Commercial Fixed Dose Combination Drugs in Indian Market.

Objective: In-vitro determination of antimicrobial activities of five different fixed dose combination drugs (FDC). Material and methods: Five antibiotic combination tablets [Cefadroxil plus Clavulanic acid (500:125 mg), Cefuroxime plus Clavulanic acid (500 : 125 mg), Cefixime plus Clavulanic acid (200 : 125 mg), Cefixime plus Ofloxacin (200 : 200 mg) and Cefixime plus Azithromycin (200 : 500 mg)] were tested for antimicrobial action against different microorganisms after their successful incubation for 18 hours at 37°C. Broth dilution or agar dilution methods were used to evaluate susceptibility of microorganisms. Results: 􀁺 Minimum inhibitory concentration (MIC) of Cefadroxil in combination with clavulanic acid reduced by 25%, 29%, 85% and 50%, against S. aureus, K. pneumonia, H. influenzae and S. pneumoniae respectively. 􀁺 No reduction was seen in MIC of Cefuroxime against S. aureus, K. pneumonia, H. influenzae, E. coli or S. pneumoniae after addition of Clavulanic acid. 􀁺 MIC of Cefixime against S. pneumoniae reduced from 0.2 to 0.14 when combined with Clavulanic acid. 􀁺 MIC of Ofloxacin against K. pneumoniae, E.coli and S. pneumoniae reduced by 82%, 50% and 85% respectively in combination with Cefixime. 􀁺 MIC of Cefixime against E.coli, S. typhi and S. pneumoniae reduced by 95%, 90% and 40% respectively in combination with Ofloxacin. 􀁺 MIC of Cefixime against S. typhi and S. pneumoniae reduced by 33.33% and 60% respectively with addition of Azithromycin. 􀁺 MIC of Azithromycin against S. typhi reduced by 70% after addition of Cefixime. Conclusion: Combination of Cefixime and Ofloxacin improves susceptibility of E. coli and S. pneumoniae. Addition of Azithromycin or Ofloxacin improves susceptibility of S. typhi against Cefixime. MIC of Azithromycin also improves with addition of Cefixime. Addition of Clavulanic acid improves antimicrobial action of Cefixime against S. pneumoniae and Cefadroxil against S. aureus, K. pneumonia, H influenza and S. pneumonia.

Validated chromatographic and spectrophotometric methods for analysis of some amoebicide drugs in their combined pharmaceutical preparation

This work is concerned with development and validation of chromatographic and spectrophotometric methods for analysis of Mebeverine HC1 [MEH], Diloxanide furoate [DF] and Metronidazole [MET] in Dimetrol tablets -spectrophotometric and RP-HPLC methods using UV detection. The developed spectrophotometric methods depend on determination of MEH and DF in the combined dosage form using the successive derivative ratio spectra method which depends on derivatization of the obtained ratio spectra in two steps using methanol as a solvent and measuring MEH at 226.4-232.2 nm [peak to peak] and DF at 260.6-264.8 nm [peak to peak]. While MET concentrations were determined using first derivative [[1]D] at lambda = 327 nm using the same solvent. The chromatographic method depends on HPLC separation on ODS column and elution with a mobile phase consisting water: methanol: triethylamine [25: 75: 0.5, by volume, orthophosphoric acid to pH =4]. Pumping the mobile phase at 0.7 ml min[-1] with UV at 230 nm. Factors affecting the developed methods were studied and optimized, moreover, they have been validated as per ICH guideline and the results demonstrated that the suggested methods are reproducible, reliable and can be applied for routine use with short time of analysis. Statistical analysis of the two developed methods with each other using F and student's-t tests showed no significant difference

Desarrollo del método de análisis para glifos tabletas / Development of the method of analysis for glyphos tablets

Se desarrolla un método analítico a un polifármaco que contiene ácido glutámico, fitina y calcio glicerofosfato, el cual se caracterizó y cuantificó en la formulación (tabletas). Se emplean para su cuantificación distintos métodos, entre ellos volumétricos y colorimétricos. Se realiza un estudio estadístico de los resultados, los cuales se encuentran dentro de los límites permisibles para los medicamentos de esta fórmula farmacéutica. Se plantea que el método analítico propuesto resulta rápido, fácil y reproducible