Deliberate self-poisoning A toxicological study

The present work was designed to detect drugs and chemicals commonly ingested by suicidal patients. This study was carried out on 100 male and female patients treated from deliberate self- poisoning. Biological samples [20 ml blood, 50 ml urine and 100 ml gastric contents] were collected from every patient. Toxicological analysis was done by enzyme multiplied immunoassay technique [EMIT] and fluorescent immunoassay system [TDX-FLX] for qualitative and quantitative detection. Thin layer chromatography [TLC] was used for the confirmation of EMIT negative results and the detection of insecticides and drugs, which can not be detected by EMIT ad TDX- FLX. From the results obtained it was concluded that the detection of drugs and chemicals used by self-poisoned patients needs more than one analytical method to give accurate results

Distributions of some insecticides in body fluids and tissues n decedents intoxicated with these chemicals

This paper described the body fluids and tissue distribution of four insecticides; one belongs to organochlorine compounds [endosulfan] and three organophosphate insecticides [malathion, chlorpyrifos and diazinon], in four suicidal human cases in Kuwait, who died after ingestion of insecticidal preparations containing these chemicals. The cases were autopsied and distribution of these chemicals were studied in stomach contents, blood, urine, bile and different tissues. Gas chromatography ion trap detector [GC-ITD] was used as analytical tool for the identification and quantitation of insecticides during this work. The study revealed higher concentrations of these insecticides in the stomach contents. Higher concentrations of malathion and endosulfan were detected in bile. Diazinon and chlorpyrifos were found at higher level in spleen. The least amount of these insecticides was recorded in muscle tissues

Prueba de Clements en contenido gástrico de recién nacidos en la predicción de madurez pulmonar / Clements' test in gastric aspirate of newborn infant inb the prediction of pulmonary maturity

Se estudió la madurez pulmonar neonatal mediante la prueba rápida de Clements para surfactante en 52 muestras de contenido gástrico de recién nacidos por intervención cesárea, obtenidas en los primeros minutos de vida. El aspecto físico, el grado de acidez, la citología y la prueba de Clements dieron resultados similares en estas muestras que en otras de líquido amniótico tomadas precozmente durante la cesárea. En el período neonatal ninguno de los 47 recién nacidos con prueba de Clements positiva en el contenido gástrico sufrió síndrome de dificultad respiratoria en contraste con 4 de 5, con resultados intermedios o negativos

Effect of ginger & garlic on DNA content of gastric aspirate.

DNA content of gastric aspirates was studied before and after intragastric infusions of three doses of ginger (2, 4, 6 g) and four doses of garlic (0.3, 0.75, 1.5, 3 g) on different days in volunteers. Only one dose was administered on any day. The mean changes of DNA-p/min in gastric aspirates after intragastric infusions of 2 and 4 g ginger were -1.37 +/- 2.3 and 6.74 +/- 3.06 respectively, which were not significant statistically. However, 6 g ginger given intragastrically showed a mean significant increase in DNA-p/min of 3.23 +/- 1.02 (P less than 0.05). Intragastric infusion of 0.3 g uncooked garlic showed a non-significant increase in DNA-p/min of 0.307 +/- 0.59. On the other hand, infusion of 0.75, 1.5 and 3 g of uncooked garlic each caused significant increase in DNA-p/min of 5.47 +/- 1.63 (P less than 0.01), 10.42 +/- 3.46 (P less than 0.01) and 29.26 +/- 4.55 (P less than 0.001) respectively. Infusion of 3 g of cooked garlic also showed significant increase in DNA-p/min of 21.43 +/- 4.62 (P less than 0.001). There was no significant difference between the effect of cooked and uncooked (3 g) garlic. Ginger in quantities of 6 g or more and garlic in quantities of 0.75 g or more cause a significant increase in exfoliation of gastric surface epithelial cells in human subjects.