Effect of some chemical agents on the viability of cysticercus bovis

The effect of eight chemicals; vinegar, Allium sativum [crude garlic], Acacia auriculiformis, lemon juice, praziquantel, pumpkin, perosan and yomesan on the viability of cysticerci of Taenia saginata in vitro was investigated. The minimum exposure times required for cysticerci to be non-evaginable for the eight chemicals were 5, 10, 25, 40, 55, 75, 90 and 105 minutes, respectively. The best one was vinegar and the least effective was yomesan

Observations on the effects of cysticercosis immune sera on cysticercosis by scanning electronmicroscopy.

Two antigens of Taenia solium cysticercus, cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used respectively to immunize rabbits in order to obtain immunosera. The CMA immunoserum added to culture medium with cysticerci limited the activities of the bladder worms. By using the scanning electronmicroscope, we could observe particulate deposits on the surface of the scolices, suckers and necks of the worms. The CFA immunoserum group showed similar changes but the deposit was less than that on the worms in the former group and appeared mainly on the cystic wall. After adding complement to the two groups mentioned above, we found that the microcilia on the surface of the worms were swollen and were seriously damaged. The worms treated with praziquantel were damaged over large area of their surfaces and were affected deep into their tissues. The damaged parts of the worms were quite different between the two groups. CMA is secreted by the living worms and therefore the serum antibodies are more effective than CFA in anti-parasite activity.

Methyl 5(6)-(alpha-hydroxyphenyl methyl) benzimidazole-2-carbamate and cysticercosis: chemotherapeutic and electron microscopic studies.

Methyl 5(6)-(alpha-hydroxyphenyl methyl) benzimidazole-2-carbamate, a major metabolite of mebendazole was evaluated against Cysticercus fasciolaria (larval form of Taenia taeniaeformis) in rats. The metabolite was assessed in various doses. A regimen of 50 mg/kg x 10 (ip), given one day apart, was found to be most effective and killed all the mature cysticerci. On developing cysts, the treatment was initiated in two schedules; 5 days prior to (d-5 to d-1) and 5 days after (d + 6 to d + 10) administration of T. taeniaeformis eggs to rats. The later protocol with 100 mg/kg x 5 dose (ip) resulted in 95% inhibition in the establishment of cysticerci. Activity of mebendazole against mature cysts was parallel to metabolite whereas against developing cysts, it was inferior. The time related topographical changes that occurred in mature C. fasciolaris after treatment with metabolite (50 mg/kg x 10, ip, one day apart) were observed by scanning electron microscopy. There was loss of contractivity, gradual disappearance of microtriches and progressive degeneration of tegument. Similar changes were noticed with mebendazole. The possession of better efficacy and higher safety range [Indian J Exp. Biol, 25 (1987) 871], suggests that the metabolite can be a potential anthelmintic for man and animals.

Cysticercus bovis: I. Ensaios de evaginaçäo / Cysticercus bovis: I. Assays of evagination

Em provas de evaginaçäo com Cysticercus bovis, obtidos de infecçöes experimentais em bezerros, foram utilizadas bile bovina natural, bile suína natural e bile bovina desidratada em variadas associaçöes como ácido clorídrico, glucose peptona e água destilada. Os melhores resultados foram obtidos com bile bovina e bile suína a 15% em água destilada e com bile bovina desidratada a 4% em água destilada. Para as condiçöes em que foi realizado o trabalho puderam ser tiradas as conclusöes abaixo: 1. A evaginaçäo de Cysticercus bovis ocorre em níveis similares nos meios à base de bile natural de bovinos e suínos; 2. A bile bovina desidratada a 4% é melhor estimulante para a evaginaçäo do que a bile natural; 3. Dentro dos níveis estudados, os percentuais de evaginaçäo crescem à medida que se aumenta a proporçäo de bile, natural ou desidratada, no meio

Neurocisticercosis, tratamiento con prazicuantel / Neurocysticercosis, treatment with praziquantel

Se ha encontrado cisticercosis en el sistema nervioso central en el 2.3 a 6.3% y 0.7% de necropsias efectuadas en México y Colombia respectivamente; mientras que de 82.700 realizadas entre 1918 a 1965 en la Universidad de California, Estados Unidos, se presentaron 17 casos; sin embargo, el número se incrementó de manera considerable debido a la inmigración de latinoamericanos en los últimos años. También se ha informado en Europa del este, India, Sudamérica siendo la infección parasitaria más común del sistema nervioso central (SNC)