RESUMEN
Abstract Purpose: To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell Methods: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies. Results: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury. Conclusion: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.
Asunto(s)
Animales , Masculino , Daño por Reperfusión/cirugía , Tejido Adiposo/citología , Lesión Renal Aguda/cirugía , Riñón/irrigación sanguínea , Ratas Endogámicas Lew , Obstrucción de la Arteria Renal/cirugía , Factores de Tiempo , Daño por Reperfusión/patología , Distribución Aleatoria , Reproducibilidad de los Resultados , Resultado del Tratamiento , Ultrasonografía Doppler en Color , Trasplante de Células Madre Mesenquimatosas/métodos , Lesión Renal Aguda/patología , Riñón/patología , NecrosisRESUMEN
Particularmente, a utilização de vários tipos de drogas que diminuem os efeitos deletérios do binômio isquemia-reperfusão, tem tornado-se foco de vários estudos experimentais visando possíveis aplicações clínicas. O objetivo do presente estudo foi avaliar os efeitos da deferoxamina na isquemia e reperfusão sobre o fígado remanescente após ressecção hepática parcial a 70 por cento, avaliando-se váriáveis bioquímicas do sangue: aspartato aminotransferase e alanina aminotransferase;.A amostra de 34 ratos foi dividida em grupos: Grupo HP (n = 8) - submetidos a hepatectomia parcial (HP) a 70 por cento; Grupo HPD (n = 4) - submetidos a administração de deferoxamina (40 mg/kg) e HP a 70 por cento; Grupo HPI (n = 7) - hepatectomizados (HP a 70 por cento) e submetidos a isquemia (40 minutos); Grupo HPID (n = 7) - semelhante ao anterior, porém recebendo previamente deferoxamina; Grupo C (n = 8) - controle, submetido a operação simulada para HP a 70 por cento. A análise estatística entre os diversos grupos foi feita pelos testes de Kruskal - Wallis e de Mann - Whitney, com nível de significância de 5 por cento. Portanto, houve aumento significativo das aminotransferases nos animais submetidos a hepatectomia e a isquemia. Esse aumento foi inibido pela deferoxamina.