RESUMEN
An extracellular β-agarase was purified from
Asunto(s)
Adaptación Fisiológica/fisiología , Agar/metabolismo , Glicósido Hidrolasas/metabolismo , Pseudoalteromonas/enzimología , Regiones Antárticas , Adaptación Fisiológica/genética , Proteínas Bacterianas/metabolismo , Frío , Disacáridos/biosíntesis , Sedimentos Geológicos/microbiología , Glicósido Hidrolasas/aislamiento & purificación , Hidrólisis , /genéticaRESUMEN
1. The effects of catbolite inactivation upon the trehalose pathway linked to maltose utilization were investigated in Saccharomyces cerevisiae. Mutant strains devoid of UDPG-trehalose synthase activity were used in this study. 2. Trehalose accumulation was also susceptible to catabolite inactivation as has been reported for the carrier protein, one of the components of the maltose system. Reversibility was only achieved when incubation with glucose did not exceed 5 min and was dependent upon protein sunthesis