RESUMEN
OBJECTIVE: This study aimed to develop a new histological scoring system for use in a partial-thickness cartilage repair animal model. Although previous papers have investigated the regeneration of articular cartilage, the good results achieved in small animals have not been replicated in large animal models or humans, possibly because of the frequent use of models with perforation of the subchondral bone plates. Partial-thickness lesions spare the subchondral bone, and this pattern is the most frequent in humans; therefore, new therapies should be tested using this model. However, no specific histological score exists to evaluate partial-thickness model results. METHODS: Histological sections from 30 ovine knees were reviewed to develop a new scoring system. The sections were subjected to H&E, Safranin O, and Masson's trichrome staining. RESULTS: This paper describes a new scoring tool that is divided into sections in detail: repair of tissue inside the lesion, cartilage around the lesion and degenerative changes at the base of the lesion. Scores range from 0 to 21; a higher score indicates better cartilage repair. DISCUSSION: Unlike existing tools, this new scale does not assign points for the positioning of a tidemark; we propose evaluation of the degenerative changes to the subchondral bone and calcified cartilage layer. It is necessary to remove the whole joint to access and study the evolution of the lesion as well as the surrounding tissue. CONCLUSION: This article emphasizes the importance of a partial-thickness animal model of cartilage repair and presents a new histological scoring system.
Asunto(s)
Animales , Regeneración/fisiología , Cartílago Articular/lesiones , Cartílago Articular/patología , Ingeniería de Tejidos/métodos , Modelos Animales de Enfermedad , Estándares de Referencia , Factores de Tiempo , Biopsia , Huesos/fisiología , Huesos/patología , Ovinos , Enfermedades de los Cartílagos/fisiopatología , Enfermedades de los Cartílagos/patología , Reproducibilidad de los Resultados , Condrocitos/fisiología , Condrocitos/patología , Miembro PosteriorRESUMEN
Las lesiones provocadas por la práctica deportiva han aumentado significativamente. Esta revisión enfatiza las lesiones osteocartilaginosas que se producen especial o exclusivamente en niños y adolescentes, cuyo diagnóstico hasta hace algunos años era clínico y la contribución de las imágenes se limitaba a estudios radiológicos simples. Los avances de otras técnicas, especialmente el ultrasonido y la resonancia magnética, han llegado a ser actualmente el método primario de elección ante la sospecha o evidencia clínica de una lesión de partes blandas u osteocartilaginosas.