RESUMEN
ABSTRACT PURPOSE: To investigate the effects of amifostine on bacterial translocation and overgrowth in colonic flora after acute radiation enteritis in a rat model. METHODS: Thirty-two female Wistar albino rats were divided into four groups: Group-1 (n=8): only normal saline was administered intraperitoneally. Group-2 (n=8): first serum saline was administered intraperitoneally and 30 minutes later 20 Gy radiation was applied to abdominopelvic region. Group-3 (n=8): only amifostine 200 ml/kg was administered intraperitoneally and radiation was not applied. Group-4 (n=8): first amifostine 200 ml/kg was administered intraperitoneally and 30 minutes later 20 Gy radiation was applied to abdominopelvic region. On the 5th day after radiation, samples of mesenteric lymph tissues and cecal contents were taken by laparotomy for microbiological culture. RESULTS: Intraperitoneal amifostine administration significantly decreased the bacterial overgrowth related to radiation in colon but did not significantly decrease the bacterial translocation. CONCLUSİON: Although not providing a full protection on the damaged mucosal barrier, amifostine significantly decreased the bacterial overgrowth in the cecal content after high dose radiation. There is a need to find out appropriate amifostine dose under different radiation applications avoiding bacterial translocation in gastrointestinal system.
Asunto(s)
Animales , Femenino , Traumatismos Experimentales por Radiación/microbiología , Protectores contra Radiación/farmacología , Amifostina/farmacología , Traslocación Bacteriana/efectos de los fármacos , Enteritis/inducido químicamente , Enterobacteriaceae/efectos de la radiación , Dosis de Radiación , Traumatismos Experimentales por Radiación/prevención & control , Ciego/efectos de la radiación , Ciego/microbiología , Ratas Wistar , Enteritis/microbiología , Enteritis/prevención & control , Enterobacteriaceae/fisiología , Linfa/microbiologíaRESUMEN
Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.
Asunto(s)
Enterobacteriaceae/enzimología , Enterobacteriaceae/fisiología , Glucosa 1-Deshidrogenasa/genética , Factores de Crecimiento Nervioso , Phaseolus/crecimiento & desarrollo , Phaseolus/microbiología , Análisis por Conglomerados , Biología Computacional , Citosol/química , ADN Bacteriano/química , ADN Bacteriano/genética , Enterobacteriaceae/genética , Enterobacteriaceae/aislamiento & purificación , Glucosa 1-Deshidrogenasa/química , Modelos Moleculares , Datos de Secuencia Molecular , Filogenia , Conformación Proteica , Estructura Terciaria de Proteína , Fósforo/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/crecimiento & desarrollo , Quinonas/análisis , Análisis de Secuencia de ADN , Homología de SecuenciaRESUMEN
Interactions of Proteus morganii, Proteus mirabilis, Proteus sp. Klebsiella oxaenae and Serratia marcescens isolated from vegetable salads of mass feeding systems with Salmonella ferlac (a new subgenus VI of Salmonella) isolated from a hostel cook's hands and lizard droppings were recorded following in-vitro nephelometric analysis. Nephelometric analysis revealed inhibition of S. ferlac by all the tested isolates from fifth hour of mixed culture interaction. K. oxaenae was the strong inhibitor.