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1.
Journal of Shahrekord University of Medical Sciences. 2010; 12 (2): 7-14
en Persa | IMEMR | ID: emr-105705

RESUMEN

Vitamin A is involved in vertebrate morphogenesis, growth, cellular differentiation, and tissue homeostasis. Vitamin A and its metabolites are essential for adequate embryo development. Electromagnetic fields [EMFs] have been used effectively to treat some diseases, such as certain musculoskeletal and chondrogenic disorders. In the present study, we aimed to investigate the synergistic effects of EMFs and Vitamin A on limb bud development in Balb/C mice in vivo. The Balb/C mice were used as experimental model. After mating, the pregnant mice were divided randomly into 3 groups as control, sham and experimental groups. Control and experimental groups received a single dose injection of Vitamin A [15000IU/Kg] on 10.5[th] gestational day, interaperitonealy. In addition, animals in experimental group were exposed to EMFs [50 Hz /100 gauss, for 4[th] during three days]. The animals were killed on the 15.5[th] day of gestation and were submitted to caesarian section. The obtained fetuses were examined externally with an analyzed under a stereoscopic microscope and were photographed. Data were analyzed statistically by t-test and ANOVA and Ktuskal-Wallis. Morphological and histological examinations showed significant changes in limb buds as compared with sham exposed and control groups. In both fore and hind limb buds significant decrease obtained in proximo-distal [P-D] and anterior posterior [A-P] axes [P<0.05]. Chondrocytes counts revealed a significant delay in the development of chondrocytes in experimental groups as compared with sham exposed and control groups [P<0.05]. Synergistic effects of vitamin A and extremely low frequency electromagnetic field [50Hz] cause delay in development


Asunto(s)
Animales de Laboratorio , Esbozos de los Miembros/efectos de los fármacos , Vitamina A , Campos Electromagnéticos , Ratones Endogámicos BALB C , Condrogénesis , Sinergismo Farmacológico , Distribución Aleatoria
2.
Experimental & Molecular Medicine ; : 656-664, 2009.
Artículo en Inglés | WPRIM | ID: wpr-10780

RESUMEN

Curcumin is a well known natural polyphenol product isolated from the rhizome of the plant Curcuma longa, anti-inflammatory agent for arthritis by inhibiting synthesis of inflammatory prostaglandins. However, the mechanisms by which curcumin regulates the functions of chondroprogenitor, such as proliferation, precartilage condensation, cytoskeletal organization or overall chondrogenic behavior, are largely unknown. In the present report, we investigated the effects and signaling mechanism of curcumin on the regulation of chondrogenesis. Treating chick limb bud mesenchymal cells with curcumin suppressed chondrogenesis by stimulating apoptotic cell death. It also inhibited reorganization of the actin cytoskeleton into a cortical pattern concomitant with rounding of chondrogenic competent cells and down-regulation of integrin beta1 and focal adhesion kinase (FAK) phosphorylation. Curcumin suppressed the phosphorylation of Akt leading to Akt inactivation. Activation of Akt by introducing a myristoylated, constitutively active form of Akt reversed the inhibitory actions of curcumin during chondrogenesis. In summary, for the first time, we describe biological properties of curcumin during chondrogenic differentiation of chick limb bud mesenchymal cells. Curcumin suppressed chondrogenesis by stimulating apoptotic cell death and down-regulating integrin-mediated reorganization of actin cytoskeleton via modulation of Akt signaling.


Asunto(s)
Animales , Embrión de Pollo , Antiinflamatorios no Esteroideos/farmacología , Apoptosis/efectos de los fármacos , Células Cultivadas , Condrogénesis/efectos de los fármacos , Curcumina/farmacología , Citoesqueleto/efectos de los fármacos , Esbozos de los Miembros/citología , Células Madre Mesenquimatosas/citología , Proteínas Proto-Oncogénicas c-akt/metabolismo
3.
Investig. andin ; 10(17): 86-95, sept. 2008.
Artículo en Español | LILACS | ID: lil-519600

RESUMEN

Antecedentes: la tosferina es una enfermedad respiratoria aguda de una alta transmisibilidad sobre todo en ámbitos intrafamiliares o intradomiciliarios.En febrero de 2008, fue informado un brote de tosferina con casos confirmados por nexo epidemiológico, algunos de los cuales no presentaban vacunación certificada y se carecía de claridad sobre sus características clínico-epidemiológicas para determinar el comportamiento de la enfermedad.Materiales y Métodos:estudio descriptivo de corte transversal.Se estableció la definición de caso confirmado por laboratorio y confirmado por nexo epidemiológico; además, se realizó búsqueda activa comunitaria e institucional, barrido de vacunación y monitoreo rápido de coberturas de vacunación y establecimiento de medidas quimio-profilácticas.Resultados:se confirmó un caso por laboratorio y 4 por nexo epidemiológico.Período de incubación =16,2 días; tasa de ataque total para las dos viviendas implicadas 33 por ciento (5/15).De las 52 casas visitadas, 42 fueron efectivas, no se presentó ausentismo de niños en las instituciones visitadas y se evidenció 100 por ciento de cobertura para DPT.De 24170 registros revisados, 3227 tenían diagnóstico compatible, 17 fueron clasificados como probables de tosferina, 7 investigados.Conclusiones:existen dificultades en la captación de casos de tosferina, subnotificación al SIVIGILA y dificultades en el diagnóstico por laboratorio para el aislamiento de la bacteria; se hace necesario reforzar la vigilancia del evento por parte de todas instituciones de salud y fortalecer la capacidad del laboratorio de salud pública...


Asunto(s)
Humanos , Esbozos de los Miembros/inmunología , Tos Ferina
4.
Korean Journal of Obstetrics and Gynecology ; : 2069-2073, 2003.
Artículo en Coreano | WPRIM | ID: wpr-21083

RESUMEN

Fetus in fetu is a very rare congenital abnormality in which one vertebrate fetus is enclosed within the abdomen of another fetus. With advancing ultrasound imaging technique, a few case of prenatal diagnosis is reported recently. A case of fetus in fetu with 6 X 6 X 4 cm sized cystic mass in the fetal retroperitoneum was diagnosed prenatally using ultrasound and confirmed by ultrasound and computer tomogram after delivery. This mass was removed completely from delivered baby and finally confirmed fetus in fetu by pathologic examination. Solid portion in this mass was composed of vertebral organization with limb bud, well-developed organ system.


Asunto(s)
Abdomen , Anomalías Congénitas , Feto , Esbozos de los Miembros , Diagnóstico Prenatal , Ultrasonografía , Vertebrados
5.
Korean Journal of Orthodontics ; : 667-676, 1996.
Artículo en Inglés | WPRIM | ID: wpr-651793

RESUMEN

We have examined the in vitro stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells using micromass culture, Our results indicate that limb bud mesenchyme cells as early as stage 16 by Carnegie system (37 days), well before the initiation of in vivo chondrogenesis, have chondrogenic potential which is expressed in micromass culture, These results are correlated with stage-related chondrogenic potential of human limb bud in vivo as a result of Alcian blue staining. The proliferation of chondrogenic cells increased in the first 3 days after culture and then decreased. These results were correlated with the cell cycle analysis of which the number of G degrees/G1 phase increased markedly after 3 days of culture, while the percentage of cells in S phase was decreased, On the other hand, it was rarely differentiated in the mandible. We examined the effects of two PKC modulators such as phorbol 12-myristate 13-acetate (PMA), a potent activator of PKC, and staurosporine (STSN), an inhibitor of PKC. PMA inhibited the chondrogenesis, whereas STSN promoted the chondrogenesis in a dose dependent manner. In addition, PMA exerted no inhibitory effect when the cells were pretreated for 24 h with STSN, implying that the chondrogenic events might be settled at an early step in vitro and PKC may act as a negative modulator, Collectively, these results demonstrate, for the first time, the stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells and the role of PKC during chondrogenesis in vitro & in vivo.


Asunto(s)
Humanos , Azul Alcián , Ciclo Celular , Condrogénesis , Estructuras Embrionarias , Extremidades , Mano , Esbozos de los Miembros , Mandíbula , Mesodermo , Proteína Quinasa C , Proteínas Quinasas , Fase S , Estaurosporina
6.
Korean Journal of Physical Anthropology ; : 145-155, 1990.
Artículo en Coreano | WPRIM | ID: wpr-60617

RESUMEN

Three cases of human embryo of Carnegie stage 13 were described. The CR length of these embryos were 3.0-5.3mm. The authors made a reconstruction model using photograph, photocopy and computer. These embryos were characterized externally by 4 limb buds and 4 pairs of branchial arches, and internally closed otic pits, appearance of venous valves, septum primum and foramen primum in the heart, beginning of the right and left lung buds, appearance of lens disk.


Asunto(s)
Humanos , Región Branquial , Estructuras Embrionarias , Corazón , Esbozos de los Miembros , Pulmón , Válvulas Venosas
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