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1.
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (3): 445-450
en Inglés | IMEMR | ID: emr-142602

RESUMEN

Recent approach in treatment and drug development suggested that the control of oxidative stress in malarial infected patients may be an added advantage. In this study, effect of methanolic leaf extract of Sphenocentrum jollyanum pier [S. jollyanum] on liver damage, markers of oxidative stress and alteration in lipid profile in P. berghei infected mice was assessed. Oxidative stress was induced by intravenously inoculation of mice with 1 x 107 sporozoites P. berghei. Treatment of parasitized mice with leaf extract of S. jollyanum had a significant [p<0.05] reductions in elevated levels of total protein, globulin, AST, ALT, ALP, GGT and total bilirubin, serum, kidney and liver malondialdehyde [MDA] concentrations, but caused a significant [p<0.05] increased in the activities of serum and liver catalase [CAT], superoxide dismutase [SOD] and glutathione [GSH] level when compared with parasitized non-treated group [PNT]. The extract treated group also showed significant [p<0.05] improvement in the levels of HDLc, total cholesterol, LDL and reduction in triglyceride compared with parasitized non treated group. Our results revealed that the protective capacity and antioxidant activity of the extract is dose dependant. The findings suggest that antioxidant property of Sphenocentrum jollyanum leave extract might be an added advantage to it anti-malarial activity


Asunto(s)
Femenino , Animales de Laboratorio , Menispermaceae/química , Ratones , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Hojas de la Planta/química , Plasmodium berghei/efectos de los fármacos , Distribución Aleatoria , Esporozoítos/efectos de los fármacos , Hígado/efectos de los fármacos , Insuficiencia Hepática , Colesterol/metabolismo , Plantas Medicinales
2.
Southeast Asian J Trop Med Public Health ; 2004 Sep; 35(3): 540-6
Artículo en Inglés | IMSEAR | ID: sea-34115

RESUMEN

This study describes the development of Cryptosporidium parvum in MDCK, MA-104, Hep-2 and Vero cell lines. Differences in susceptibility, infectivity, and the methodology of excystation were determined. Various solutions were considered to determine the factors which enhanced the excystation (eg with and without sodium hypochlorite, trypsin or sodium taurocholate). It was shown that the sporozoites could be excysted in media either with or without trypsin and sodium taurocholate, but the number of sporozoites in the latter solution was less than the former one. Only oocysts digested by sodium hypochlorite and trypsin can enter the culture cells. Numerous meronts and oocysts were demonstrated and persisted for 9 days. Asexual stages were not observed in MA-104. Only few oocysts could be detected 1-3 days post-inoculation. There was a significant difference between the number of oocysts, which invaded MDCK, MA-104, and Hep-2 cells. MDCK gave the highest susceptibility to oocyst invasion among the three cell lines and asexual stages were also found. Among the 25 isolates, which had been cultivated, 23 isolates could infect MDCK and Hep-2. Only 2 isolates could not infect the MDCK cell. These 2 isolates could infect the Vero cell and yielded high numbers of trophozoites. Praziquantel (PZQ), doxycycline, and paromomycin (PRM) were tested on the infecting parasites. The drugs were added either with the inoculum or 24 hours after inoculation. None of them was effective, including PRM, which had been previously reported as effective.


Asunto(s)
Síndrome de Inmunodeficiencia Adquirida/complicaciones , Animales , Antihelmínticos/administración & dosificación , Antibacterianos/administración & dosificación , Técnicas de Cultivo de Célula , Línea Celular/efectos de los fármacos , Criptosporidiosis/complicaciones , Cryptosporidium parvum/efectos de los fármacos , Heces/parasitología , Humanos , Oocistos/efectos de los fármacos , Hipoclorito de Sodio/farmacología , Esporozoítos/efectos de los fármacos , Ácido Taurocólico/farmacología , Tripsina/farmacología
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