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Biol. Res ; 49: 1-10, 2016. ilus, graf
Artículo en Inglés | LILACS | ID: biblio-950834

RESUMEN

BACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-1α) and up-regulation of HIF-1α-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells. RESULTS: We found that CoCl2 induced the stabilization of HIF-1α and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1α stabilization, activity not previously reported for AGE and SAC. CONCLUSIONS: Obtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1α and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditions


Asunto(s)
Animales , Ratas , Extractos Vegetales/farmacología , Apoptosis/efectos de los fármacos , Cisteína/análogos & derivados , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/efectos de los fármacos , Ajo/química , Antioxidantes/farmacología , Sales de Tetrazolio , Hipoxia de la Célula/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Análisis de Varianza , Células PC12 , Especies Reactivas de Oxígeno/análisis , Cobalto , Cisteína/farmacología , Citometría de Flujo , Formazáns
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